Microbial preparation for mineralizing and consolidating zinc ions and application method for microbial preparation

A technology of microbial preparations and application methods, applied in the field of microbial preparations, can solve problems such as zinc ions that have not been reported, and achieve the effects of not being prone to secondary pollution, clean environment, and low cost

Inactive Publication Date: 2012-07-04
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • Microbial preparation for mineralizing and consolidating zinc ions and application method for microbial preparation
  • Microbial preparation for mineralizing and consolidating zinc ions and application method for microbial preparation
  • Microbial preparation for mineralizing and consolidating zinc ions and application method for microbial preparation

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Embodiment 1

[0027] 1 Substrate amount and composition determination

[0028] In order to determine the ability of the strain to decompose the substrate urea, at room temperature, according to the principle of color development between p-dimethylaminobenzaldehyde solution and urea, the degree of urea decomposition was determined by spectrophotometry.

[0029] 。

[0030] According to the above results, it can be seen that the decomposition ability of urea per 1L of bacterial solution is about 1.1mol-1.2mol. However, in the above experiments, urea was added to the reaction system in the form of 8 mol / L liquid, but in practical application, the form of solid is more convenient for practical application. At the same time, the factor of temperature should also be considered.

[0031] Add excess urea (solid) to 100 mL of the cultured bacterial solution, place them in 30°C and 15°C environments respectively, and observe the change of urea concentration.

[0032] Table 3 Effect of ambient t...

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Abstract

The invention discloses a microbial preparation for mineralizing and consolidating zinc ions and an application method for the microbial preparation. The microbial preparation is prepared by compounding 1 liter of bacillus pasteurii liquid and 60 to 80 grams of solid urea, wherein the optical density (OD) of the bacillus pasteurii liquid is 1.2. The microbial preparation is mixed uniformly and sprayed in a metal zinc ion contamination region, wherein the application amount is based on the zinc ion contamination level; urea is added according to the molar ratio of the urea to the zinc ions of 1:1; and the bacillus pasteurii liquid is added based on the mass of the urea. Microorganisms are decomposed under the enzymatic action induced by urea to generate carbonate ions, so that the zinc ions in the solution can be effectively mineralized and consolidated. Environmental impact is low and secondary pollution is avoided.

Description

technical field [0001] The invention relates to a microbial preparation for mineralizing and consolidating zinc ions, which uses the enzymatic action of microorganisms induced by urea to decompose and produce carbonate ions, which can decompose Zn in the environment 2+ Effectively consolidates to the mineral state. Background technique [0002] The pollution of metals and heavy metals has become a global problem. Whether in soil or water, various metals and heavy metals have entered the bottom of the human food chain, seriously threatening human health. In recent years, metal and heavy metal pollution Governance has become an important research direction for governments and research institutions in various countries. [0003] There are many traditional physical and chemical methods for treating metals and heavy metals, but there are disadvantages such as large investment, high energy consumption, difficult operation, and easy to produce secondary pollution to varying degree...

Claims

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Application Information

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IPC IPC(8): A62D3/02C09K17/00B09C1/10A62D101/43
Inventor 钱春香许燕波
Owner SOUTHEAST UNIV
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