Check patentability & draft patents in minutes with Patsnap Eureka AI!

Method for removing bacteria in water body by using zero-valent nanometer ferromagnet through separation and inactivation

A zero-valent nano-iron, magnetic separation technology, applied in the fields of magnetic field/electric field water/sewage treatment, sterilization/microdynamic water/sewage treatment, etc., to achieve the effect of strong reaction ability, broad application prospects and large specific surface area

Inactive Publication Date: 2012-07-04
PEKING UNIV
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many studies have focused on the inactivation effect and mechanism of zero-valent nano-iron on microorganisms, but few studies have used its magnetic properties to remove pollutants, especially the removal of microorganisms in water

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 0

[0026] Example 1 Zero-valent nano-iron removes Bacillus subtilis from water

[0027] (1) NaBH 4 The solution was added dropwise to an equimolar concentration of Fe(NO 3 ) 3 9H 2 In the O solution, the magnetic particles are stirred rapidly and accompanied by the release of hydrogen gas. The synthesized zero-valent iron nano-particles are collected by centrifugation and washed twice with sterile deionized water, but this step can be omitted according to the situation. Finally, sterile deionized water is added to make Concentration is the zero-valent nano-iron suspension of 5mg / ml for subsequent use;

[0028] (2) Add 1.8ml of 5mg / ml zero-valent nano-iron suspension into a 15ml sterile test tube (add 1.8ml sterile deionized water to the control test tube), then add 0.2ml of Bacillus subtilis to a concentration of 10 4 -10 10 Inject the CFU / ml bacterial solution below the zero-valent nano-iron suspension liquid level, mix thoroughly and vibrate vigorously with a vortex instru...

Embodiment 2 0

[0030] Example 2 Zero-valent nano-iron removes Escherichia coli (Escherichia coli) from water

[0031] (1) NaBH 4 The solution was added dropwise to an equimolar concentration of Fe(NO 3 ) 3 9H 2 In the O solution, the magnetons are stirred rapidly and accompanied by the release of hydrogen gas. The synthesized zero-valent nano-iron particles are collected by centrifugation and washed twice with sterile deionized water. Finally, sterile deionized water is added to form a zero-valent Iron suspension for use;

[0032] (2) Add 1.8ml of 5mg / ml zero-valent nano-iron suspension into a 15ml sterile test tube (add 1.8ml sterile deionized water to the control test tube), and then add 0.2ml of Escherichia coli to a concentration of 10 4 -10 10 Inject the CFU / ml bacterial solution below the zero-valent nano-iron suspension liquid level, mix thoroughly and vibrate vigorously with a vortex instrument at 3200rpm for 5 minutes, then take 100 μl of the bacterial solution from the test tu...

Embodiment 3

[0034] Example 3 Zero-valent nano-iron removes bacteria in domestic wastewater

[0035] (1) NaBH 4 The solution was added dropwise to an equimolar concentration of Fe(NO 3 ) 3 9H 2 In the O solution, the magnetons are stirred rapidly and accompanied by the release of hydrogen gas. The synthesized zero-valent nano-iron particles are collected by centrifugation and washed twice with sterile deionized water. Finally, sterile deionized water is added to make a 5 mg / ml zero-valent nano-iron suspension. spare;

[0036](2) Add 1.8ml of 5mg / ml zero-valent nano-iron suspension into a 15ml sterile test tube (add 1.8ml sterile deionized water to the control test tube), and then add 0.2ml of bacterial concentration to 10 4 CFU / ml of domestic wastewater was injected below the liquid level of the zero-valent nano-iron suspension, mixed thoroughly and aerobically shaken vigorously at 3200 rpm for 5 minutes with a vortex meter, and 100 μl of the bacterial solution from the test tubes of t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for removing bacteria in a water body by using a zero-valent nanometer ferromagnet through separation and inactivation. The method comprises the following steps of: (1) synthesizing nanoscale zero-valent iron by adopting a chemical reaction method and preparing a suspension solution from the nanoscale zero-valent iron with a certain concentration; (2) sufficiently mixing the nanoscale zero-valent iron suspension with bacteria liquid or a bacteria-contained waste water sample, and severely shaking for certain time in an aerobic condition; (3) standing a reacted mixture system beside a magnet for certain time; (4) taking the residual water sample out until magnetic grains and bacteria adsorbed by the magnetic grains are gathered at one side of the magnet, so that the bacteria in the water body are separated and inactivated. By using the own magnetism, the adsorption property and the inactivation effect of the nanoscale zero-valent iron grains to the bacteria, the bacteria in the water body are efficiently removed and inactivated. The method disclosed by the invention has simple and rapid steps, is economic and harmless, and has very wide application prospect in sewage purification and waste water treatment.

Description

technical field [0001] The invention relates to a method for using chemically synthesized zero-valent nano-iron particles to magnetically separate and inactivate bacteria in water bodies, and is mainly used in processes such as sewage purification and waste water treatment. Background technique [0002] Lack of safe and clean water remains a serious global challenge. According to the report of the World Health Organization, those bacteria that spread through water can pose a great threat to human health. In addition to the health risks caused by direct contact or ingestion of polluted water bodies, there are greater indirect risks of exposure through airborne pathogens contained in biological sludge generated by processes such as sewage treatment, which are often underestimated in current environmental protection practices even ignored. Clearly, pretreatment of drinking water or wastewater is necessary to reduce its associated biological health hazards. [0003] Tradition...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C02F1/50C02F1/48
Inventor 要茂盛陈琦李菁
Owner PEKING UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com