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Oligopeptide with breast cancer resistant activity and application thereof

An anti-breast cancer and oligopeptide technology, applied in the field of biomedicine, can solve the problems of high cost and long cycle, and achieve the effects of small molecular weight, inhibition of proliferation, and easy industrialization

Inactive Publication Date: 2013-12-18
ZHUHAI JINLONG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006]In the early stage, bioactive peptides were mainly isolated and expanded from natural animals, plants, insects and other organisms, with long cycle and high cost

Method used

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  • Oligopeptide with breast cancer resistant activity and application thereof
  • Oligopeptide with breast cancer resistant activity and application thereof
  • Oligopeptide with breast cancer resistant activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Design and synthesis of oligopeptides

[0025] 1. Extract the main international antimicrobial peptide database (http: / / aps.unmc.edu / AP / main.php; http: / / penbase.immunaqua.com / ; http: / / www.bbcm.univ.trieste.it / ; http: / / www.imtech.res.in / raghava / antibp / ) registered in the antibacterial peptide sequence, analyze the corresponding relationship between its primary structure and activity, and design artificial short peptide sequence.

[0026] . Utilize the protein analysis database http: / / www.expasy.org / to analyze the similarity between the oligopeptide sequence with known activity and the secondary structure of the designed oligopeptide sequence, and correct the designed sequence in combination with other physicochemical parameter predictions.

[0027] , The oligopeptide sequence is sent to the biological company for synthesis or the full sequence is synthesized by an automatic oligopeptide synthesizer. The purity of the HPLC test should be greater than 96%, and t...

Embodiment 2

[0031] Identification of oligopeptides

[0032] P1221 oligopeptide was hydrolyzed with 5.7mol / L HCl for 16 hours, and amino acid analysis was carried out. It was known that it contained 9 kinds of amino acids, including His, Asn, Tyr, Gly, Phe, Val, Pro, Ser, and Leu, and the molar ratio was His:Asn :Tyr:Gly:Phe:Val:Pro:Ser:Leu = 1:1:1:1:1:1:1:1:1. After amino acid sequence analysis, the sequence is His-Asn-Tyr-Gly-Phe-Val-Pro-Ser-Leu. A peak was obtained by electrospray mass spectrometry, and its molecular weight was 1033.15.

Embodiment 3

[0034] Detection of tumor suppressor activity of P1221 oligopeptide in vitro by MTT assay

[0035] 2.1 Materials and reagents

[0036] 1) Oligopeptide P1221 was synthesized by Shanghai Sangon Bioengineering Technology Service Co., Ltd.

[0037] ) Human breast cancer cell lines MCF-7, MDA-MB-231, and human normal breast cell lines MCF-10A were purchased from the Cell Resource Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

[0038] ) RPMI 1640 medium, DMEM medium, and newborn calf serum are all products of American GIBCO Company.

[0039] ) dimethyl sulfoxide (DMSO), tetramethylazozole (MTT) and trypsin are products of Sigma.

[0040] 2.2 Experiment

[0041] 1) Dilute the P1221 oligopeptide to an initial concentration of 1 mM with a diluent (sterile PBS, or sterile water, or the corresponding cell culture medium), filter and sterilize, and freeze at -20°C after aliquoting.

[0042] ) Cell culture: Cancer cells are cultured...

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Abstract

The invention discloses an oligopeptide with breast cancer resistant activity. The oligopeptide has a sequence of His-Asn-Tyr-Gly-Phe-Val-Pro-Ser-Leu. The oligopeptide can selectively inhibit the growth of in-vitro breast cancer cells under low concentration and does not have any influence on normal breast cells. In-vivo experiments show that the oligopeptide has a strong effect of inhibiting the growth and proliferation of cancer cells and has an obvious dose-effect relationship. Because of a safe, efficient and ideal anti-cancer effect, the oligopeptide can be used for preparing anti-cancer medicines and has a good application prospect.

Description

technical field [0001] The present invention relates to oligopeptide and its application, in particular to an oligopeptide with anti-breast cancer activity and its application, which belongs to the field of biomedicine. Background technique [0002] Breast cancer is the main malignant tumor that endangers women's health. About 1.2 million women in the world develop breast cancer every year, accounting for 18% of all tumors in women. The 5-year survival rate of breast cancer is about 50%-60%, nearly 50% of patients relapse and metastasize after treatment, and the average survival time of patients with advanced breast cancer is only 18-30 months. Breast cancer treatment methods include surgery, radiotherapy, chemotherapy, endocrine therapy and other traditional treatments. However, the risk of surgical treatment is high, the trauma to the human body is large, the patient's immunity is reduced, and the resistance to the disease is reduced. A series of surgical complications ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A61K38/08A61P35/00
Inventor 夏环玲党宁宁邵云霞胡海波
Owner ZHUHAI JINLONG BIOTECH CO LTD