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Special culture medium for strict anaerobe detection of beer

A technology of anaerobic bacteria and culture medium, which is applied in the field of special culture medium for the detection of strict anaerobic bacteria in beer

Inactive Publication Date: 2012-07-04
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the temperature drops to about 50°C, use it on a flat plate

Method used

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  • Special culture medium for strict anaerobe detection of beer
  • Special culture medium for strict anaerobe detection of beer
  • Special culture medium for strict anaerobe detection of beer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] The preparation method of embodiment 1 medium:

[0009] Weigh yeast extract: 0.5g, peptone: 0.3g, sodium lactate: 1.7g, ascorbic acid: 0.1g; sodium thiosulfate (Na2S2O4 5H2O): 0.01g, lead acetate: 0.02g, methylene blue (Methylene blue) : 0.0002g, phenylethyl alcohol (PEA): 0.2g, agar powder (ager) 1.5g.

[0010] After accurately weighing each component, put it into a conical flask with a certain volume (according to the experimental needs), add 100ml of distilled water, mix the components, heat and boil on an electric stove for 3-5 minutes (the culture medium turns from blue to colorless) , until the components are completely dissolved. When the temperature drops to about 50°C, it can be used on a flat plate. The plate must be used in a strictly anaerobic anaerobic box or anaerobic tank or anaerobic tube.

Embodiment 2

[0011] The using method of embodiment 2 culture medium:

[0012] The inoculated bacteria were two strains of Pectinatus cerevisiiphilus and Pectinatus frisingensis and lactic acid bacteria, the inoculation gradient was 10-3 gradient, and anaerobic culture was carried out at 28°C for two weeks. Lead acetate reacts with hydrogen sulfide released by pectin bacteria to form lead sulfide, forming black colonies. Since lactic acid bacteria do not produce hydrogen sulfide, their colonies remain milky white. Therefore, strict anaerobic bacteria can be clearly distinguished and identified. The specific colony color and effect can be found in the accompanying drawings of the instructions.

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Abstract

The invention provides a special culture medium for strict anaerobe detection of beer. The characteristic that strict anaerobes generate unique metabolites is utilized, ingredients in the special culture medium generate screening culture media of color development reaction, and lead acetate takes reaction with hydrogen sulfide released by pectinatus to generate lead sulfide for generating black bacterial colonies. Other pollution bacteria do not generate hydrogen sulfide, and the bacterial colonies of the other pollution bacteria are still milk-white. Therefore, the strict anaerobes can be obviously distinguished and identified, the detection and identification on the strict anaerobes by the special culture medium is simpler and clearer, the quality control and the detection level of products can be enhanced, the possibly generated strict anaerobe pollution risk can be treated, and the effect is obvious.

Description

technical field [0001] The invention relates to a special culture medium for beer strict anaerobic bacteria detection, which belongs to the field of microbial fermentation. Background technique [0002] Strictly anaerobic bacteria are more reported in foreign countries, and there are few reports of detection in the domestic beer industry. One is that there are very few detection contents specifically for strict anaerobic bacteria in China, and the second is that strict anaerobic bacteria have relatively strict requirements for oxygen, and the quality control level of oxygen for early beer brewing in China is relatively low. At present, with the improvement of the quality control level of beer brewing in China, the amount of dissolved oxygen in beer is getting lower and lower. The absolute anaerobic bacteria that were only cultivated abroad in the past, such as Pectinatus sp. and Megasphaera cerevisiae The risk of contamination continues to increase. The growth of strictly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/01
Inventor 王德良宋绪磊郭立芸林智平贾凤超袁雪萍
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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