21 results about "Strict anaerobe" patented technology

This invention relates to a strain of Bacillus cereus and its applications, specifically Bacillus cereus (… Bacillus cereus Bacillus cereus (W1) was deposited at the China Center for Type Culture Collection on July 21, 2022, with accession number CCTCC NO: M 20221147. Bacillus cereus W1 can grow and ferment high-yield butyric acid under non-strict anaerobic conditions, and has the advantages of strong oxygen tolerance and high butyric acid yield.

The present invention relates to a method for anaerobic detoxification of coal chemical wastewater, and belongs to the technical field of anaerobic detoxification of coal chemical wastewater. The method comprises pre-treatment to regulate the redox potential of the wastewater to a range of 80mV to 50mV; inoculating a mixed bacterial community of thiocyanate-degrading bacteria and phenol-hydrolyzing bacteria on a polyurethane foam carrier to form a layered biofilm; controlling the redox potential in stages, adding sodium formate under microaerobic conditions to preferentially degrade thiocyanate to produce bicarbonate; adding nano-zero-valent iron to reduce catechol and adsorb cyanide; and adding ethanol and humic acid under strictly anaerobic conditions to degrade phenols. Through the decoupling of bacterial metabolic pathways, the synergistic detoxification of metabolites and the targeted detoxification of nanomaterials, the problems of redundancy of traditional multi-stage reactor equipment, bacterial competitive inhibition and toxic accumulation of intermediate products are solved, and efficient step-by-step degradation of thiocyanate and phenols is achieved in a single reactor, the biodegradability of the wastewater is significantly improved, and a low-toxic environment is provided for subsequent treatment.

The present application relates to a kind of lignocellulose as raw material fermentation production butyric acid method, comprising the following steps: (1) lignocellulose raw material is pretreated, enzymolysis, filtration obtains enzymolysis liquid;(2) the enzymolysis liquid is detoxified, separates out the precipitate after pH adjustment as fermentation medium;(3) the seed liquid of bacillus cereus (W1) Bacillus cereus ) W1 is inoculated into fermentation medium in non-strict anaerobic environment, and fermentation production butyric acid.The present application uses lignocellulose as raw material, utilizes bacillus cereus (W1) Bacillus cereus ) W1 in non-strict anaerobic environment fermentation production butyric acid, with butyric acid yield high, product selectivity is high, fermentation cost is low and so on.

The invention relates to the technical field of biomedicine and microorganisms, and provides an intestinal flora excrement sample preserving fluid which comprises a buffer system, a chelating agent, a reductive anaerobic protective agent, a cell lysis-bacteriostatic agent and a biomacromolecular stabilizer, wherein the buffer system is a combination of a Tris-HCl buffer solution and sodium chloride, the chelating agent is ethylenediamine tetraacetic acid disodium salt, the reductive anaerobic protective agent is L-cysteine hydrochloride, the cell lysis-bacteriostatic agent is sodium lauroyl sarcosinate, and the biomacromolecular stabilizer is a combination of trehalose and glycerin. The invention also provides a preparation method of the preserving fluid. According to the invention, the microbial DNA and community structure in the excrement can be stably preserved at room temperature for a long time without a cold chain; meanwhile, the preservation solution can effectively protect DNA of strict anaerobic bacteria from oxidative degradation, flora composition is prevented from shifting, and the reliability of subsequent molecular detection and analysis is finally ensured.

The application belongs to the technical field of environmental microorganisms, and particularly relates to a strain of Rhodococcus and application thereof. Rhodococcus qingshengii The strain is Rhodococcus qingshengii (abbreviated as strain R), which has been preserved in the China General Microbiological Culture Collection Center on May 13, 2025, and the strain preservation number is CGMCC No.34527. The high-efficiency fermentation iron reduction ability of the strain under low-temperature (4 DEG C) and strict anaerobic conditions can not only be applied to the repair of contaminated sites in cold regions, especially those deep anaerobic environments with low-temperature stress, such as high-latitude groundwater, polar soil and even glacial sediment environments, but also help to understand the influence of extreme low temperature on the fermentation iron reduction process of microorganisms. This study helps to develop innovative in-situ bioremediation technology to solve a series of trivalent iron pollution problems in high-cold anaerobic environments.

The invention discloses a high-osmotic-pressure-tolerant high-yield succinic acid recombinant engineering bacterium as well as a method and application thereof, aiming at the technical bottlenecks that in the existing process of producing succinic acid from escherichia coli, the growth of thalli is limited, the yield is reduced and the like due to high osmotic pressure of a fermentation environment after a product is neutralized by a neutralizing agent. The invention provides a genetically engineered bacterium for improving the yield of succinic acid. Wherein the engineering bacterium is constructed by overexpressing rpsR, cutA, gltI or queG genes from the engineering bacterium in escherichia coli ZX11. The genetic modification effectively enhances the osmotic pressure stress resistance of the strain, so that the viability and succinic acid production capacity of the strain under a high osmotic pressure fermentation condition are improved. Under strict anaerobic fermentation conditions, the highest succinic acid yield of shake-flask fermentation of the engineering bacteria reaches 30.92 g / L, and compared with an original strain, the succinic acid yield is remarkably improved.

The invention discloses anaerobic clostridium aerovorans freeze-dried powder as well as a preparation method and application thereof, belongs to the field of microbial fermentation engineering, and solves the problems of low freeze-drying survival rate and small viable count of clostridium aerovorans in the freeze-drying process, and the activity of the clostridium aerovorans freeze-dried powder reaches 63.2%; the clostridium aerovorans freeze-dried powder is stable in storage, and the activity retention rate of the clostridium aerovorans freeze-dried powder still reaches 76.5% after the clostridium aerovorans freeze-dried powder is stored for 3 months at 4 DEG C. Compared with the prior art, the clostridium aerovorans freeze-dried powder prepared by the method has the advantages of good stability, long storage time and high activity; the clostridium aerovorans freeze-dried powder prepared by the method disclosed by the invention can be directly applied to inoculated fermentation in a fermentation tank, so that not only is the time required by anaerobic pre-culture of clostridium aerovorans seeds saved, but also the equipment investment required by strict anaerobic pre-culture is reduced.

The invention provides a device and a method for culturing high-abundance free anaerobic ammonium oxidation bacteria, which are used for solving the problems of slow growth, insufficient abundance, difficulty in obtaining free bacteria and overlong period in the existing culture. The device comprises a reactor, a stirring system, a water inlet and outlet system, an air inlet system, a temperature system, a liquid level system, a pH / DO monitoring system and a PLC control system. The method comprises the steps of culture medium preparation, reactor starting, inlet water concentration gradient regulation and control, sludge age management, reaction condition control and membrane module maintenance. A strict anaerobic environment is established through nitrogen stripping and mixed gas supply, and rapid adaptation and enrichment are realized by combining gradually increasing ammonia nitrogen and nitrite nitrogen concentrations; membrane effluent and sludge discharge are utilized to maintain flora advantages, temperature, pH, dissolved oxygen and stirring conditions are regulated and controlled, and conversion from an aggregation state to a free state is promoted, so that high-abundance and uniform flora with higher metabolism efficiency is obtained in a short period, and a stable bacterium source is provided for deep denitrification of sewage.

This invention belongs to the field of microbial technology, and discloses a highly efficient anaerobic fungal strain for degrading lignocellulose in the rumen of Dulong cattle, its cultivation method, and its applications. The strain is *Neisseria gonorrhoeae*. Joblinomyces apicalis DLM2, deposited at the China Center for Type Culture Collection (CCTCC) under accession number CCTCC NO: M 20252927, was isolated from the rumen of Dulong cattle and can be stably cultured under strictly anaerobic conditions. This strain possesses a single-centered cell line and filamentous rhizoids, enabling it to efficiently degrade lignocellulose through a synergistic effect of physical colonization and enzymatic degradation. It exhibits broad adaptability to natural straws such as rice straw and bamboo branches, as well as purified substrates such as microcrystalline cellulose and cellobiose. Its enzyme production exhibits substrate-inducible properties, and fermentation produces various metabolites including formic acid, acetic acid, and ethanol, demonstrating high lignocellulose degradation efficiency. This invention relates to an inoculum containing this strain and its application in lignocellulose degradation, roughage fermentation, agricultural straw resource utilization, and biomass conversion, providing new strain resources and technical support for the efficient degradation and resource utilization of lignocellulose.

The invention relates to the technical field of probiotics, in particular to a preparation method and application of a bangia fusco-polysaccharide and ackermania composition. The preparation method comprises the following steps: degassing a BFP extracting solution; pressurizing nitrogen to press the ackermania suspension liquid into the BFP extracting solution, supplementing nitrogen, and stirring nitrogen to obtain a mixed solution; cooling and spraying the mixed solution to form powder embedded particles; pressing the powder embedded particles into a freeze dryer through pressurized nitrogen to remove moisture to obtain a porous bangia fusco-polysaccharide and ackermania composition; the whole preparation process is under nitrogen protection; the bangia fusco-polysaccharide is combined with Ackerman's bacteria to improve the structure and composition of intestinal flora and increase colonization of Ackerman's bacteria, so that the effect of treating ulcerative colitis is achieved; the gas dissolved in the BFP extracting solution is nitrogen, so that a strict anaerobic condition is formed; the composition with a loose porous structure is convenient and rapid to rehydrate and restore activity; the BFP can be conveniently used as the unique carbon source of Ackerman's bacteria to enhance the growth, proliferation and adhesion planting of Ackerman's bacteria.

A kind of compound Clostridium butyricum oral liquid fermentation method based on step-by-step inoculation and static maturation, Clostridium butyricum oral liquid is combined fermentation by strict anaerobic Clostridium butyricum, facultative anaerobic Bacillus and heterotrophic anaerobic lactic acid bacteria.The probiotic compound culture of the present application not only solves the problem of oxygen in culture medium inhibiting normal growth of Clostridium butyricum, but also can mutualistic symbiosis between different strains to strengthen the gastrointestinal health function of oral liquid, esterification and alcoholization reaction can occur between metabolic products produced by Clostridium butyricum, facultative anaerobic Bacillus and heterotrophic anaerobic lactic acid bacteria, so that the odor produced by Clostridium butyricum can be eliminated, the smell and taste of oral liquid are better, organic acids produced by flora can reduce the pH of oral liquid, which is beneficial to prolong the shelf life of product and reduce storage cost, so that the product stability and functionality of oral liquid are superior to bifidobacterium oral liquid.

The invention belongs to the technical field of environmental microorganisms, and particularly relates to rhodococcus and application thereof. The strain is Rhodococcus qingshenii (short for strain R), the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 13, 2025, and the preservation number of the strain is CGMCC No. 34527. The invention further discloses a preparation method of the Rhodococcus qingshenii strain. The strain has high-efficiency fermentation iron reduction capacity at low temperature (4 DEG C) and under strict anaerobic conditions, and can be applied to remediation of polluted sites in cold regions, especially deep anaerobic environments with low-temperature stress, such as high-latitude underground water, polar soil and even glacier sediment environments; meanwhile, the influence of extreme low temperature on the microbial fermentation iron reduction process is also known. The research is helpful for developing an innovative in-situ bioremediation technology so as to solve a series of ferric iron pollution problems in an alpine anaerobic environment.

The present invention provides a process for spray freezing or spray freeze drying under conditions of very low oxygen pressure, such as under essentially anaerobic conditions, and particularly a process for spray freezing strict anaerobic microorganisms (bacteria) under conditions where the level of oxygen is very low, or essentially anaerobic. Further, the invention provides a product achievable by the processes and an apparatus usable in the process.

The invention discloses a complete mixing continuous flow anaerobic ammonia oxidation sludge reinforced granulation device and method, and belongs to the technical field of wastewater biological denitrification. The device takes a complete mixing continuous flow reactor as a core, and is provided with a monitoring control platform, a temperature control device, a pH regulating agent tank and a nutrient solution agent tank. A magnetic suspension stirring device is arranged at the bottom of the reactor and comprises a stirring rotor arranged in an inner cavity of the reactor and a magnetic suspension motor fixed to the outer side of the bottom of the reactor, the magnetic suspension motor drives the stirring rotor to rotate in a magnetic coupling mode without a penetrating shaft, and a strict anaerobic and high-shear mixing environment is constructed for anaerobic ammonium oxidation bacteria. A detachable sludge blocking baffle is arranged on an effluent weir at the upper part of the reactor, and sieve plates with different apertures can be replaced according to operation stages, so that efficient interception and backflow of granular sludge with different particle sizes under a continuous flow condition are realized. On-line monitoring and automatic control are integrated, the anaerobic ammonia oxidation sludge granulation process is effectively promoted, and it is guaranteed that the system stably runs for a long time under the continuous flow condition.

The invention discloses a production process of millet-flavor baijiu, and belongs to the technical field of baijiu, and the production process of the millet-flavor baijiu is characterized by comprising the following steps: S1, soaking millet until the water content is 35-45%; s2, steaming the millet and the auxiliary materials respectively, heating the millet to 80-85 DEG C, scattering high-temperature-resistant alpha-amylase, uniformly stirring, heating to 90-95 DEG C, and keeping the temperature for 30-60 minutes; s3, spreading and airing; s4, a fermentation and saccharification stage: mixing the cooked millet with aerobic mould, and carrying out aerobic fermentation at the temperature of 30-35 DEG C; a main fermentation stage: adding saccharomyces cerevisiae into the millet, and carrying out anaerobic fermentation at 28-30 DEG C; in an aroma-producing stage, adding anaerobic bacteria into the millet, and performing strict anaerobic fermentation at normal temperature to obtain fermented grains; s5, distilling, namely adding the steamed auxiliary materials into the fermented grains, uniformly stirring, distilling, and performing three-stage condensation at the temperature of 80-85 DEG C in the first stage, 60-75 DEG C in the second stage and 40-45 DEG C in the third stage; and S6, ageing to achieve the effect of improving the taste and aroma of the Baijiu.

The invention relates to the technical field of oral liquid, in particular to angelica sinensis blood-enriching oral liquid and a preparation process thereof. Comprising the following steps: preparing an angelica sinensis water extract, preparing an angelica sinensis fermentation liquor and preparing the angelica sinensis blood-enriching oral liquid. The lactic acid bacteria and the bifidobacteria are cultured, the symbiotic relationship is established according to a specific proportion for fermentation, the number of the lactic acid bacteria is dominant, acid can be rapidly produced, the pH value of the environment is reduced, stricter anaerobic and acid environments are created, the growth of the strictly anaerobic bifidobacteria is facilitated, the lactic acid bacteria can rapidly start acidification, and the fermentation efficiency is improved. In addition, the lactic acid bacteria can quickly utilize simple carbohydrates, the bifidobacteria utilize complex carbohydrates, the lactic acid bacteria and the bifidobacteria cooperate to metabolize, the substrate utilization rate is increased, organic acids generated by the lactic acid bacteria and the bifidobacteria jointly create a bacteriostatic environment, and the antibacterial activity of the bifidobacteria is improved. And the content of nutrient substances in the Chinese angelica fermentation liquor is increased by more comprehensively converting the substrate.

The object of the present patent application relates to the field of hexitols for use in the prevention and / or treatment of diseases and / or symptoms caused by anaerobic pathogenic microorganisms, and more particularly to monoanhydrohexitols, dianhydrohexitols or combinations thereof for use in the prevention and / or treatment of said diseases and / or symptoms. In one embodiment, this object is an isosorbide for use in the prevention and / or treatment of diseases and / or conditions associated with strictly anaerobic pathogenic bacteria such as fusobacterium nucleatum, Porphyromonas gingivalis, Wehonor, and Micromonas minuta.

The invention belongs to the field of microorganism and molecule detection, and particularly relates to a quantitative detection method of caproic acid bacteria. Aiming at the problems of long detection time, tedious operation steps, low flux, high requirements on strict anaerobic conditions, easy infection of infectious microbes and the like of a traditional detection method, the invention provides a novel caproic acid bacteria detection method, a qPCR specific primer pair is designed based on recA genes of to-be-detected caproic acid bacteria, and the caproic acid bacteria are detected by using the qPCR specific primer pair. According to the primer pair and the method provided by the invention, the identification efficiency of the caproic acid bacteria can be effectively improved, a caproic acid bacteria screening method is optimized, and a quicker detection method is provided for related research of the caproic acid bacteria.

The application belongs to water pollution treatment technology, and particularly relates to an anaerobic dehalogenation microbial preservation method and application. The dehalogenation microorganism is resuspended in a embedding agent solution, and then the resuspension is added dropwise into a crosslinking agent, and then is reacted in an ice bath under anaerobic conditions for 10-20 min; after the reaction, the crosslinking is continued for 24-36 h under anaerobic conditions at 4-6 DEG C, and the preservation of the anaerobic dehalogenation microorganism is realized after the crosslinking. The anaerobic dehalogenation microorganism is fixed, and through centrifugation, resuspension and crosslinking into a ball under strict anaerobic conditions, the immobilized bacterial agent is obtained while the bacterial solution is concentrated and the cells are wrapped, the tolerance of the dehalogenation microorganism to oxygen is significantly improved, the problem that the dehalogenation microorganism is easy to be exposed to air and lose the degradation activity in the form of culture solution is overcome, and great convenience is provided for the implementation of in-situ bioremediation engineering of a contaminated site.

The invention discloses a method for realizing DNRA based on ferrate sludge wall breaking and carbon source utilization, and belongs to the technical field of sewage treatment and recycling. The method comprises the following steps: S1, in a strict anaerobic environment, adding ferrate into the excess sludge, and rapidly stirring to realize wall breaking of the sludge and release intracellular organic matters; s2, carrying out solid-liquid separation on the reacted mixture, collecting supernate, and diluting the supernate to a specific chemical oxygen demand (COD) concentration to obtain recovered carbon source liquid; and S3, adding the recovered carbon source liquid into the nitrate nitrogen-containing wastewater, regulating the carbon-nitrogen ratio and oxidation-reduction potential (ORP) of the system, and reducing the nitrate nitrogen into ammonia nitrogen by using functional microorganisms under anaerobic conditions to realize accumulation and recovery of the ammonia nitrogen. High-concentration carbon sources are released through efficient wall breaking of ferrate, and the problem that traditional nitrogen removal carbon sources are deficient is solved; the process of directionally inducing dissimilatory nitrate to be reduced into ammonium (DNRA) is regulated and controlled through environmental parameters, low-concentration nitrate nitrogen is converted into recoverable ammonia nitrogen, nitrogen loss is avoided, and the method has the advantages of efficient nitrogen removal and resource recovery and is environmentally friendly.

The present application relates to the technical fields of microbial culture, and particularly relates to an anaerobic bacteria culture device capable of regulating anaerobic degree and a use method thereof, comprising a vacuum pump, a CO2 gas cylinder, an H2 gas cylinder, an N2 gas cylinder and an anaerobic bottle, the vacuum pump is connected with the anaerobic bottle, each gas cylinder is connected with a proportioner to control the gas proportion in the anaerobic bottle, a culture tube is arranged in the anaerobic bottle, an opening is formed in the anaerobic bottle, a pressing plate for pressing a sealing film is hinged to the anaerobic bottle, and a driving assembly for driving the culture tube to move upward is arranged on the anaerobic bottle. The present application has simple structure, convenient operation, controllable culture environment and low price, and is suitable for small-scale enterprises and laboratory students. The strict anaerobic requirement in the experiment process is achieved through vacuum negative pressure adsorption. The gas proportion is regulated through the proportioner to meet different experimental requirements. The anaerobic degree of the environment can be further determined through the anaerobic indicator arranged in the system.