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Detection method and correlated detection probe and kit for prostatic cancer related fusion gene Fish

A prostate cancer and fusion gene technology, applied in biochemical equipment and methods, microbe measurement/inspection, etc., can solve rare problems and achieve the effects of intuitive detection, simple operation, and ingenious design

Inactive Publication Date: 2012-07-04
嘉兴久泰生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As a fast, accurate and intuitive molecular genetics technique, FISH is widely used in the diagnosis and prognosis of bladder cancer, breast cancer, and blood system diseases. more common

Method used

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  • Detection method and correlated detection probe and kit for prostatic cancer related fusion gene Fish
  • Detection method and correlated detection probe and kit for prostatic cancer related fusion gene Fish
  • Detection method and correlated detection probe and kit for prostatic cancer related fusion gene Fish

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Embodiment 1

[0122] 1. source of sample material

[0123] Samples of prostate cancer tissue slices and normal prostate samples were obtained from the hospital.

[0124] 2. Dewaxing and Hydration

[0125] Deparaffinize the samples in xylene for 5 min in 1 repeat. Then hydrate in 95% ethanol and 70% ethanol twice for 5 minutes each.

[0126] 3. hybridization pretreatment

[0127] The samples were bathed in boiling water at 95°C for 10 minutes, transferred to 1×PBS and equilibrated for 5 minutes, and repeated once.

[0128] Remove the sample and digest it with pepsin for 5-15 minutes. Transfer to 1x PBS to equilibrate for 5 min.

[0129] Remove samples and fix with 1% formaldehyde for 1 min. Transfer to 1x PBS to equilibrate for 5 min.

[0130] The samples were dehydrated with 75%, 85%, and 95% graded ethanol for 5 minutes each, and dried in the air.

[0131] 4. Denaturation of tissue sections

[0132] The samples were denatured in a preheated denaturing solution at 70°C for 5 mi...

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Abstract

The invention relates to a detection method of prostatic cancer related fusion gene Fish. The detection method comprises the steps of: carrying out conventional pretreatment on prostatic cancer tissue paraffin sections, then carrying out in-situ hybridization with a two-color fusion probe, and judging whether the prostatic cancer related fusion gene exists according to the detected fluorescence signal. Preferably, the two-color fusion probe is TMPRSS2 / ERG two-color fusion probe, TMPRSS2 / ETV1 two-color fusion probe or TMPRSS2 / ETV4 two-color fusion probe, wherein the TMPRSS2, ERG, ETV1 and ETV4 are respectively nucleotide sequences shown in SEQ ID NO: 1-4. The invention also relates to a correlated two-color fusion probe and a kit. The detection method of the prostatic cancer related fusion gene Fish is ingenious in design, is simple to operate, greatly increases specificity and sensitivity, can be used for quickly, accurately and visually detecting the prostatic cancer related fusion gene, and has accurate and reliable detection result, thus the detection method can be used as a reference for diagnosing prostatic cancer by physicians, and is suitable for large-scale popularization and application.

Description

technical field [0001] The present invention relates to the technical field of detection of disease-related genes, more specifically, to the technical field of detection of prostate cancer-related fusion genes, in particular to a Fish detection method for prostate cancer-related fusion genes, related detection probes and kits. Background technique [0002] Prostate cancer (prostatic cancer, Pca) is the most common malignant tumor of the male reproductive system. In North America, the incidence of prostate cancer is second only to skin cancer, and 1 in 6 American men will suffer from prostate cancer. The death rate of prostate cancer is second only to lung cancer, and 1 in 36 American men will die from prostate cancer. In 2010, 217,730 cases of prostate cancer and 32,050 deaths were reported. The incidence of prostate cancer increases with age. In the 50-year-old population, the incidence rate is about 2.14%, and in the 70-year-old population, the incidence rate rises to 8....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 赵翊均白小萍谭睿
Owner 嘉兴久泰生物技术有限公司
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