Method for extracting wedelolactone from yerbadetajo herb

Abstract

The invention relates to a method for extracting wedelolactone from yerbadetajo herb. The method comprises the following steps of: crushing dry yerbadetajo herb, putting the crushed dry yerbadetajo herb in a supercritical fluid extraction kettle for extraction, adding alcohol liquid as an entrainer, collecting analytical liquid, extracting for 2-3 times by using n-butyl alcohol, combining extract liquor, concentrating to be 1 / 15-1 / 8 of the original volume, stirring with a little amount of polyamide resin, leading the n-butyl alcohol to be volatilized completely, adopting a dry process for polyamide chromatographic columns, eluting by using a chloroform-absolute ethyl alcohol mixed-solvent system, collecting eluent, evaporating and obtaining a product. The method has the advantages that the operation is simple, the energy consumption is less, and the prepared product is good in quality and high in content.

Description

Technical field: [0001] The invention belongs to the field of separation of natural products, and in particular relates to a method for extracting wedelolide from Eclipta chinensis. Background technique: [0002] Eclipta prostrata L., commonly known as Eclipta prostrata L., is the dry aerial part of Eclipta prostrata L., a commonly used traditional Chinese medicine for treating liver diseases. According to the "Chinese Pharmacopoeia", it has the effects of nourishing the liver and kidney, cooling blood and stopping bleeding. Modern pharmacological studies have shown that Eclipta chinensis and its extracts have strong liver protection and expansion effects. [0003] Wedelolide: Coumarin, easily soluble in ethyl acetate, n-butanol, methanol, is a derivative of coumarins. It mainly exists in the leaves of Eclipta japonica. [0004] Wu Hongxia's master's degree thesis "Research on the Quality Evaluation Method of Eclipta chinensis" pointed out that the main hepatoprotective a...

AI Technical Summary

Problems solved by technology

Such as Wu Hongxia's master's degree thesis "Research on Ecliptas quality evaluation method", the method adopted in this document is methanol immersion, the leachate is concentrated to extractum, water bath heating after water dispersion, filtered while hot, and the filtrate is extracted with ethyl acetate. The extract is recovered from ethyl acetate, concentrated to the extract, then applied to a silica gel column, eluted with chloroform-methanol (10:1), evaporated to dryness, and then recrystallized by methanol. This method has many steps, complicated operation, and repeated concentration. Large energy consumption, long methanol immersion cycle and small amount of preparation; for example, "Research on the Chemical Composition of Eclipta" published by Wu Jiang et al., the method disclosed in this document is 70% ethanol reflux extraction, and the extract is concentrated to no alcohol. Ethyl acetate extraction, the extract is separated and purified by silica gel column and SephadexLH-20 gel column respectively. This method obtains the product by repeated loading on the column. The preparation amount is small, the cycle is long, and the reflux process consumes a lot of energy

[0006] In existing patents, there is a method of chemical total synthesis to prepare wedelolide, such as "a method for preparing wedelide" (patent No. CN1566116A) disclosed by Li Chuangchuang, etc., this patent uses 3,4-dihydroxybenzene Formaldehyde and 2,4,6-trihydroxybenzaldehyde are used as raw materials to synthesize two key intermediates in a convergent manner, and the two intermediate products are then obtained by Sonogashira coupling reaction and carbonyl ring formation reaction to obtain wedelolide, which is the key in this synthetic method The intermediates are not easy to obtain, the synthesis cost is high, the operation process is cumbersome, and the intermediate products are difficult to remove