Specific primer group, kit comprising the primer group, use method and detection method

A technology of specificity and primer set, applied in the fields of virology and molecular biology, can solve the problem of rapid detection of red sea bream iridescent virus that has not been seen yet
CN102559928AInactive Publication Date: 2012-07-11OCEAN UNIV OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
OCEAN UNIV OF CHINA
Publication Date
2012-07-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a detection kit for red sea bream iridovirus, comprising a specific primer group which is designed for detecting the red sea bream iridovirus. The specific primer group is composed of the following 6 specific primers: a forward outer primer (F3), a backward outer primer (B3), a forward inner primer (FIP), a backward inner primer (BIP), a loop forward primer (LF) and a loop backward primer (LB). The kit provided by the invention adopts the loop-mediated isothermal amplification (LAMP) technology and designs 6 primers based on the full gene sequence of the main capsid protein (MCP) of the red sea bream iridovirus, so that the target fragment is subjected to circulatory displacement and amplification in an isothermal environment, the amplification of a target sequence is realized, and further, the detection purpose is achieved. The kit for detecting the red sea bream iridovirus is rapid and efficient, can meet the onsite and timely detection requirements and provides technical support for the quick detection of the red sea bream iridovirus in aquaculture industry of Chinese and international fish trade.
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Description

technical field

[0001] The invention belongs to the fields of virology and molecular biology, and in particular relates to a red sea bream iridescent virus detection kit. Background technique

[0002] Red sea bream iridovirus (RSIV) belongs to the family Iridoviridae and belongs to the genus Megalocytivirus. Japan, subsequently, broke out in East Asia and Southeast Asia one after another. When the disease breaks out in nature, most of them are very sudden, and the fatality rate is high. It is currently one of the most serious diseases for mariculture in various countries. At the same time, the disease also seriously affects the import and export trade of economic fish. The disease is listed as a disease that must be reported to the World Organization for Animal Health (OIE), and the Ministry of Agriculture of my country lists it as a second-class disease, and monitors the disease in my country's coastal waters twice a year.

[0003] Existing detection methods for red sea b...

Claims

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