Intestinal probiotic powder for abalone culture and preparation method thereof

A technology of intestinal probiotics and probiotic dry powder, which is applied in applications, animal feed, animal feed, etc., can solve the problems of probiotic outflow, single probiotic strains, and pollution of the breeding environment, achieving high pertinence and stability , The method of use is simple, and the effect of low experience requirements

Inactive Publication Date: 2012-07-18
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] (1) Probiotics isolated from other aquatic animals or water bodies are mostly used in abalone farming, and microorganisms have host specificity and environmental susceptibility, so they lack pertinence and stability and cannot produce good results
[0007] (2) The scope of screening is limited, such as focusing on the breeding environment, or directly applying the reported marine bacteria, etc., so that the probiotic s

Method used

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  • Intestinal probiotic powder for abalone culture and preparation method thereof
  • Intestinal probiotic powder for abalone culture and preparation method thereof
  • Intestinal probiotic powder for abalone culture and preparation method thereof

Examples

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Example Embodiment

[0036] Example 1

[0037] Take 2m 2 In the breeding pond, 500 adult abalones (shell length 4~5cm) need to be added about 100g probiotic powder / day as an example.

[0038] 1) After the marine bacteria Phaeobacter sp. AP1220 and Bacillus stratosphericus A3440 are activated by 2216E solid medium, they are respectively inoculated into 100ml 2216E liquid fermentation medium and cultured with shaking at 28°C for 1 to 3 days to make the cell concentration Reach logarithmic growth phase 0.5~1.0×10 9 cfu / ml;

[0039] 2) After mixing the bacterial fermentation broth, centrifuge, discard the supernatant, wash the bacterial pellet with physiological saline 2 to 3 times, and centrifuge to obtain 1 to 2 g of bacterial mud;

[0040] 3) To prepare a dry powder protective agent, 5.0g of sucrose and 2.0g of sodium alginate are required, and the volume is adjusted to 100ml with water, mixed well, sterilized at 115°C for 30min, and cooled to 30~35°C for use;

[0041] 4) Add 2ml of dry powder protective ag...

Example Embodiment

[0047] Example 2

[0048] Take 2m 2 In the breeding pond, 2000 juvenile abalones (1.5-3cm) need to be added with 50g probiotic powder / day as an example.

[0049] 1) After the marine bacteria Phaeobacter sp. AP1220 and Bacillus stratosphericus A3440 are activated by 2216E solid medium, they are respectively inoculated into 100ml liquid fermentation medium and cultured with shaking at 28°C for 1 to 3 days to make the cell concentration reach Logarithmic growth period 0.5~1.0×10 9 cfu / ml;

[0050] 2) After mixing the bacterial fermentation broth, centrifuge, discard the supernatant, wash the bacterial pellet with physiological saline 2 to 3 times, and centrifuge to obtain 1 to 2 g of bacterial mud;

[0051] 3) To prepare a dry powder protective agent, 5.0g of sucrose and 2.0g of sodium alginate are required, and the volume is adjusted to 100ml with water, mixed well, sterilized at 115°C for 30min, and cooled to 30~35°C for use;

[0052] 4) Add 2ml of dry powder protective agent to the bac...

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Abstract

The invention discloses intestinal probiotic powder for abalone culture and a preparation method thereof and relates to a live bacteria preparation for abalone culture. The intestinal probiotic powder comprises probiotic dry powder, agar powder and feed. The preparation method comprises the following steps of: respectively activating Phaeobacter sp. AP1220 and Bacillus stratosphericus A3440, which are separated from abalone intestines via a culture medium, and then inoculating the Phaeobacter sp. AP1220 and the Bacillus stratosphericus A3440 into a fermentation culture medium for shaking culture so as to enable the concentration of thalli to achieve logarithmic phase 0.5-1.0 multiplied by 10<9>cfu/ml; mixing all strain fermentation solutions, then centrifugating, abandoning supernatant fluid, and performing precipitation, washing, centrifugation and sterilization on the thalli to get bacterial sludge; preparing a dry powder protecting agent; mixing the dry powder protecting agent withthe bacterial sludge, performing a film-forming reaction, then pre-freezing a mixture and further performing freeze-drying to prepare the active probiotic dry powder with an embedding layer; and mixing the probiotic dry powder, the agar powder and the feed to prepare the intestinal probiotic powder for abalone culture.

Description

technical field [0001] The invention relates to a live bacterial preparation for abalone cultivation, in particular to an intestinal probiotic powder mainly used for abalone cultivation and a preparation method thereof. Background technique [0002] my country's abalone farming started in the late 1970s, and now it has become the world's largest abalone farming country, with an annual output value of more than 4 billion yuan, and has become an important part of my country's mariculture industry. However, with the rapid development of aquaculture, the improvement of intensification and the deteriorating coastal water quality, the outbreaks of abalone diseases are increasing, especially since 2000, the most serious outbreaks of death are during the season transition period of the grow-out stage. The incidence rate in areas with dense abalone farms is as high as 90%, causing heavy economic losses. At the same time, the abuse of antibiotics has resulted in biological resistance...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/16
Inventor 赵晶蒋庆茹柯才焕
Owner XIAMEN UNIV
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