Method for extracting anions from plant medicine by alkali fusion method performing ion chromatographic detection

A technology of ion chromatography and detection method, which is applied in the field of medicinal chemical extraction and analysis, and can solve the problems of various impurity components, high noise of instrument operation, and difficulty in popularization.

Inactive Publication Date: 2012-07-18
YUNNAN MINZU UNIV
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  • Claims
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Problems solved by technology

The pretreatment techniques for solid sample extraction include traditional extraction and new treatment methods such as accelerated solvent extraction (ASE), microwave-assisted extraction (MAE), supercritical fluid extraction (SCFE), ultrasonic extraction (UAE), etc. Although the new processing technology has the characteristics of fast extraction speed

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  • Method for extracting anions from plant medicine by alkali fusion method performing ion chromatographic detection

Examples

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Embodiment 1

[0025] Example 1: Extraction and Analysis of Inorganic Anions in Hypericum botanicals

[0026] 1. Preparation of inorganic anion extract

[0027] The raw material drug sample of Kingsberry is washed with water and then rinsed with deionized water three times, dried at 50°C until the water content is 4%, crushed to a particle size of 0.15-0.25mm, and placed in a desiccator for later use.

[0028] Weigh 0.5g of the above-mentioned herbal medicine of Goldenseal, put it into a cleaned iron crucible, add 3g of alkali flux, wherein MgO is 2g Na 2 CO 3 Mix 1g with the bulk drug, and spread 2.1g of alkali flux evenly on the surface of the mixed bulk drug, wherein MgO is 1.4g Na 2 CO 3 Put the iron crucible into an electric furnace and heat it to 600°C for 1.5 hours, transfer the sample to a beaker, add deionized water to dissolve it after cooling, and filter it with a 0.45 μm filter membrane to obtain an anion-containing extract. Take 1 mL of the extract and dilute it to 100 mL wi...

Embodiment 2

[0034] Example 2: Extraction and Analysis of Inorganic Anions in Penglaige Herbal Medicine

[0035] 1. Preparation of Inorganic Anion Extraction Solution

[0036] Wash the Penglaige raw material drug sample with water and then rinse it with deionized water for 5 times, dry it at 60°C until the water content is 3%, grind it to a particle size of 0.15-0.25mm, and put it in Put in a desiccator for later use.

[0037] Weigh 1g of Penglaige botanical medicine with the above particle size and put it into a cleaned iron crucible, add 5.1g of alkali flux wherein MgO is 3.4g Na 2 CO 3 Mix 1.7g with the crude drug, then evenly spread 4.2g alkali flux on the surface of the mixed crude drug, wherein MgO is 2.8g Na 2 CO 3 Put the iron crucible into an electric furnace and heat it to 580°C for 2 hours, transfer the sample to a beaker, add deionized water to dissolve it after cooling, and filter it with a 0.45 μm filter membrane to obtain an anion-containing extract. Take 1 mL of the ex...

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Abstract

The invention aims to provide a method for extracting anions from a plant medicine by an alkali fusion method performing ion chromatographic detection. The method comprises the following steps: mixing the plant medicine raw material and an alkali fusion agent uniformly; after firing, adding deionized water and dissolving; filtering to obtain anion-containing extracting solution; diluting the deionized water by using the extracting solution to obtain the extracting solution at the required concentration; filtering to obtain the anion-containing solution to be tested; and testing the anions of the solution to be tested by using an ion chromatographic analyzer, wherein the seven inorganic anions have high linear relationship in the range of 0.4 to 12 mg/L; the detection limit is 1.20 to 5.00 ug/L; the labeling recovery rate reaches 70 to 85 percent; and the precision is 0.67 to 5.97 percent. The invention aims to provide a method for extracting and measuring total fluorine, total chlorine, total bromine, total nitrogen, total phosphorus and total sulfur from the plant medicine by the alkali fusion method and provides basis for the growth environment of the plant medicine and research on pharmacodynamic mechanism.

Description

technical field [0001] The invention relates to an alkali fusion method for extracting anions in herbal medicines and an ion chromatography detection method, and belongs to the technical field of pharmaceutical chemistry extraction and analysis. Background technique [0002] Due to the wide variety of herbal medicines with obvious curative effects and low side effects, their clinical application has a long history. However, the complex organic and inorganic chemical components in herbal medicines have not yet been elucidated. This is the medicinal substance of herbal medicines. Major bottlenecks in basic, quality control, and pharmacological studies of action. At present, the development of herbal medicines in our country is still in the ascendant period. Therefore, the qualitative and quantitative analysis of chemical components is extremely important in the research process of herbal medicines. The combination of various chromatography and mass spectrometry is currently t...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 王红斌杨树科杨敏胡晓旭马林转张英杰郭俊明刘天成
Owner YUNNAN MINZU UNIV
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