Unlock instant, AI-driven research and patent intelligence for your innovation.

Bifidobacterium longum SQS7-31, as well as preparation method and application thereof

A technology of Bifidobacterium longum and Bifidobacterium, applied in the biological field, can solve the problem of reduced survival rate and achieve the effect of excellent tolerance and superior survival rate

Inactive Publication Date: 2012-07-25
SHANGHAI JIAO TONG UNIV
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At higher temperatures, the survival rate decreases substantially

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bifidobacterium longum SQS7-31, as well as preparation method and application thereof
  • Bifidobacterium longum SQS7-31, as well as preparation method and application thereof
  • Bifidobacterium longum SQS7-31, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: Separation and purification of bacterial strains

[0033] Take 5 grams of fresh infant feces and shake it with 50ml of feces diluent to make a homogenate, put 1ml of the feces homogenate into a test tube with 9ml of MRSC medium, adjust the pH value to 3.0, culture it anaerobically at 37°C for 24 hours, and then pick it up with an inoculation loop The bacterial solution was streaked on the MRSC solid medium plate, and cultured anaerobically at 37°C for 24h. Under sterile conditions, use an inoculation loop to pick colonies on the streaked MRSC solid medium plate, continue to streak on a new MRSC plate, place the plate in an anaerobic box, and culture it in a 37°C incubator for 72 hours. A single colony was picked for microscopic examination to confirm that the pure strain was isolated. The strain was identified as Bifidobacterium longum and named as Bifidobacterium longum SQS7-31.

Embodiment 2

[0034] Embodiment 2: Microscopic examination of bacterial strain

[0035] Under sterile conditions, inoculate Bifidobacterium longum SQS7-31 with rings, smear on glass slides, prepare slides and Gram stain, and observe the morphology of bacteria under a microscope.

Embodiment 3

[0036] Embodiment 3: strain fermentation

[0037] Use an inoculation loop to pick two rings of Bifidobacterium longum SQS7-31 from the MRSC slant, put them into a test tube containing 10ml MRSC liquid medium, and after culturing for 24 hours, insert SQS7-31 into MRSC liquid culture with 1% inoculum Medium, 37 ° C anaerobic culture for 24 hours, the Bifidobacterium longum SQS7-31 fermentation broth was obtained.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides bifidobacterium longum SQS7-31, as well as a preparation method and application thereof. The 16S rRNA (ribosomal ribonucleic acid) gene sequence of the bifidobacterium longum SQS7-31 is as shown in the figure. The bifidobacterium longum provided by the invention is of a strain separated from fresh child feces, and the fermentation culture process is as follows: performing anaerobic culture in an MRSC (DeMan-Rogosa-Sharpe cysteine) culture medium (0.05% of cysteine hydrochloride is added into MRS (DeMan-Rogosa-Sharpe)) at the temperature of 37 DEG C for 24 hours. The acid tolerance of the bifidobacterium longum SQS7-31 provided by the invention is significantly better than that of an ordinary strain, the acid tolerance has genetic stability, the tolerance to a bile salt of the bifidobacterium longum SQS7-31 is significantly better than that of the ordinary strain, and the survival rate in simulated gastric fluid has significant advantages. The bifidobacterium longum SQS7-31 can be used in the field of production of foods, health-care foods and medicaments.

Description

technical field [0001] The invention relates to biotechnology, in particular to a strain of bifidobacterium longum and its preparation method and application. Background technique [0002] The human gastrointestinal tract inhabits various bacteria, which can be divided into beneficial bacteria, harmful bacteria and neutral bacteria according to their effects on the human body. Beneficial bacteria are what we call probiotics, among which bifidobacteria and lactobacilli are the most representative probiotics. When the number of probiotics is dominant, the human body will show a healthy state. [0003] Bifidobacterium is a Gram-positive bacterium, an obligate anaerobic bacterium, which has no motile properties, does not produce spores during the growth cycle, and has no capsule and flagella. [0004] Bifidobacteria are very sensitive to changes in pH. According to literature reports, the optimum growth pH of bifidobacteria is 6.7-7.0. Once it is higher than 8.0 or lower than ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L1/29A61K35/74A61P37/02A61P35/00A61P31/00A61P29/00A61P39/06A61P3/06A61P1/00C12R1/01A23L33/00A61K35/745
Inventor 杨虹方鸣玉杭晓敏
Owner SHANGHAI JIAO TONG UNIV