Method for fast detection of illegal cooking oil
A detection method and waste oil technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., to achieve high sensitivity, wide adaptability, and strong reliability
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Embodiment 1
[0054] Embodiment 1 Commodity tea seed oil and medical silica gel comparative experiment
[0055] 1. Sample pretreatment:
[0056] Medical silica gel has been proved to be cytotoxic in the preliminary experiment, and it is used as a positive control group in this test. Toxic culture medium is to soak appropriate amount of medical silica gel in 15 ml culture medium for not less than 24 hours and store at 4°C.
[0057] The pretreatment method of the oil sample to be tested is as follows:
[0058] 1.1 The oil samples to be tested are all filtered through a 0.22 μm sterile syringe filter in an ultra-clean bench, and stored in a dark place at room temperature. Filter once unless otherwise specified.
[0059] 1.2 For the sample oil with obvious white precipitate, such as the crude waste oil sample, centrifuge at 4000 rpm for 1 hour, take the supernatant oil, and filter it in the open environment and ultra-clean bench successively with a 0.22 μm sterile syringe filter. Store in d...
Embodiment 2
[0093] Example 2 Comparative test of commercial tea seed oil and canteen waste oil
[0094] Sample pretreatment and detection methods are similar to Example 1, and the same conditions as Example 1 can be used. Sample source: Canteen waste oil was collected from Wenxing Plaza restaurant in Dushu Lake Higher Education District.
[0095] Experimental results:
[0096] 1.1 Chinese hamster ovary cells (CHO):
[0097] Compared with commercial tea seed oil, canteen waste oil was more toxic to cells after 24 hours and 48 hours of treatment. The results showed that the sensitivity of the CHO cell line to the canteen waste oil after 24 hours of culture under normal conditions was lower than that of the 2-hour group. The result is as Figure 8 shown.
[0098] Figure 8 The changes in cell morphology and Coomassie brilliant blue staining results of CHO cell lines treated with commercial tea seed oil and canteen waste oil for 24 and 48 hours. The negative control group was normal cu...
Embodiment 3
[0102] Example 3 Contrast experiment between commercial tea seed oil and crude waste oil (CHO cell line)
[0103] Sample pretreatment and detection methods are similar to Example 1, and the same conditions as Example 1 can be used. The source of the sample is rough extraction of waste oil, which was sampled by the Suzhou Dushu Lake Higher Education District Bureau of the Suzhou Municipal Public Security Bureau. It is waste oil that has not been further processed and has a peculiar smell.
[0104] The results show (such as Figure 10 As shown), after the cells were treated with crude waste oil for 24 hours, a large number of floating dead cell clumps appeared in the medium, and the cells died.
[0105] Figure 10 The changes in cell morphology and Coomassie brilliant blue staining results of CHO cell lines treated with commercial tea seed oil and crude waste oil for 24 hours. The negative control group was normal cultured cells. Cells were incubated under normal conditions ...
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