Method for detecting and analyzing lignin content of pear pulp

An analysis method, lignin technology, applied in the field of detection and analysis of lignin content in pear pulp, can solve problems such as inappropriateness, time-consuming experiment process, errors, etc., to avoid interference, facilitate solvent dissolution, and reduce connection effects Effect

Active Publication Date: 2012-07-25
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0003] At present, the analysis method of lignin content in pear fruit pulp mainly adopts the classical method for the analysis of woody or herbaceous plant stems, leaves and other materials. Since the lignin content in pear fruit pulp is relatively low, this method is applied to Lignin content analysis often causes large errors, and the experimental process takes a long time, so it is not suitable for direct analysis of pear pulp lignin content

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  • Method for detecting and analyzing lignin content of pear pulp
  • Method for detecting and analyzing lignin content of pear pulp

Examples

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example 1

[0027] Take 10g of the middle layer of pear pulp frozen for 12 hours, 2g as a group, and divide it into 5 groups. Each group is carried out according to the following steps: Homogenize at a speed of 18000r / min for 2 minutes, dry at 60°C to constant weight, crush and over 40 mesh sieve. Weigh 0.02 g of the prepared pear pulp dry powder, wash 3 times with 95% ethanol, then rinse 3 times with alcohol:n-hexane=1:2 (V / V, volume percentage) solution, and blow dry. Put it into a 10mL test tube, add 1mL of 20% bromoacetyl-glacial acetic acid solution (w / w, mass percentage) and 0.05mL of perchloric acid with a volume concentration of 70%, put a wooden stopper on the tube mouth and seal it, and place it in a constant temperature water bath at 60°C 30min, shaking the test tube every 10min. After the reaction was finished, 3 mL of NaOH with a concentration of 2 mol / L and 2 mL of hydroxylammonium hydrochloride with a concentration of 4 mol / L were added to the test tube to terminate the re...

example 2

[0029] Take 10g of the middle layer of pear pulp frozen for 24h, 2g as a group, and divide it into 5 groups, and each group is carried out according to the following steps: Homogenize at a speed of 20000r / min for 2min, dry at 60°C to constant weight, crush and over 40 mesh sieve. Weigh 0.02 g of prepared pear pulp dry powder, wash 3 times with 95% ethanol, then rinse 3 times with alcohol:n-hexane=1:2 (V / V) solution, and blow dry. Put it into a 10mL test tube, add 2mL of 25% bromoacetyl-glacial acetic acid solution (w / w) and 0.07mL of perchloric acid with a volume concentration of 70%, put a wooden stopper on the mouth of the tube and seal it, and put it in a constant temperature water bath at 70°C for 30min. Shake the test tube every 10 min. After the reaction was finished, 3.5 mL of NaOH with a concentration of 2 mol / L and 2 mL of hydroxylammonium hydrochloride with a concentration of 4 mol / L were added to the test tube to terminate the reaction. Transfer the reaction solut...

example 3

[0031] Take 10g of the middle layer of pear pulp frozen for 18h, 2g as a group, and divide it into 5 groups, and each group is carried out according to the following steps: Homogenize at a speed of 20000r / min for 3min, dry at 60°C to constant weight, crush and over 40 mesh sieve. Weigh 0.02 g of prepared pear pulp dry powder, wash 3 times with 95% ethanol, then rinse 3 times with alcohol:n-hexane=1:2 (V / V) solution, and blow dry. Put it into a 10mL test tube, add 1mL of 30% bromoacetyl-glacial acetic acid solution (w / w) and 0.05mL of perchloric acid with a volume concentration of 70%, put a wooden stopper on the mouth of the tube and seal it, and put it in a constant temperature water bath at 80°C for 40min. Shake the test tube every 10 min. After the reaction, 4 mL of NaOH with a concentration of 2 mol / L and 3 mL of hydroxylammonium hydrochloride with a concentration of 4 mol / L were added to the test tube to terminate the reaction. Transfer the reaction solution into a 10mL...

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Abstract

The invention discloses a method for detecting and analyzing lignin content of pear pulp. The method comprises the following steps of: freezing the pear pulp to be detected, homogenizing, crushing, sieving, cleaning to remove soluble impurities, and digesting and hydrolyzing pear pulp cells by use of acidification acetyl bromide to dissolve lignin; then adding sodium hydroxide and hydroxylamine hydrochloride into the digested and hydrolyzed solution to terminate the reaction, and centrifuging the solution to eliminate the influence of impurities and precipitates on absorbance measurement; and finally measuring the absorbance value at 280 nanometers by using an ultraviolet spectrophotometer, and substituting the absorbance value into a formula to calculate the lignin content. An analysis method which has the advantages of accurate analysis result, high analysis speed, simplicity in operation, high sensitivity and good reproducibility is provided for the relatively low lignin content of the pear pulp.

Description

technical field [0001] The invention relates to a method for detecting and analyzing lignin content in pear pulp. Background technique [0002] The deposition of lignin on the cell wall of parenchyma cells in pear fruit caused secondary thickening of the cell wall to form stone cells. The size, number and density of stone cells in pear pulp all affect the quality of pear fruit, and are an important indicator of pear fruit quality. Therefore, there is a need for a correct and suitable method to detect the lignin content in pear fruit pulp. [0003] At present, the analysis method of lignin content in pear fruit pulp mainly adopts the classical method for the analysis of woody or herbaceous plant stems, leaves and other materials. Since the lignin content in pear fruit pulp is relatively low, this method is applied to The analysis of lignin content often causes large errors, and the experimental process takes a long time, so it is not suitable for direct analysis of lignin c...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N1/42
Inventor 蔡永萍王斌邱景香林毅金青魏晓飞
Owner ANHUI AGRICULTURAL UNIVERSITY
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