Process for preparing board-spectrum antibiotics by immune induction biotechnology

Abstract

The invention discloses a process for preparing board-spectrum antibiotics by immune induction biotechnology, and particularly relates to a process for preparing board-spectrum antibiotics by immune induction biotechnical synthesis, which employs super immunoprotein synthesis ability of silkworms and includes: allowing fifth-instar silkworms to synthetize high-quantity sericin antibacterial peptides from a middle division of silkgland and silk fibroin antibacterial peptides from a posterior division of the silkgland; regulating silk protein genes with highly bioactive agent; and performing synthesis and amplification to obtain board-spectrum antibiotics by solid-phase and liquid-phase biotechnology. By the process, biotechnical synthetic routes are simple and easy to learn, production cost is low, productivity is highly controllable, disease resistance reaches more than 80%-95%, and achievement is easy to transform and convenient for industrial production. The antibacterial peptides prepared by the process can be mixed with oil and used for soaking roots, and have a higher bacterial effect and efficient and broad-spectrum antibacterial advantages to infections caused by wilt fusarium, gram-positive bacterium, gram-negative bacterium or pathogens such as Bacillus subtilis, bacterial wilt and the like.

Description

technical field [0001] The invention relates to a method for preparing antibiotics, in particular to a method for preparing broad-spectrum antibiotics by immune induction biotechnology. Background technique [0002] Antibacterial peptides, also known as peptide antibiotics or antimicrobial peptides, are a type of active peptides produced by the biological immune system to resist external biological infection sources, and widely exist in insects, plants, animals and humans. In addition to its non-specific anti-bacteria, fungus, virus, anti-infection, and anti-disease effects, it also has anti-cell mutation and anti-tumor cells without damaging normal human cells, especially for multi-drug resistant strains. Antimicrobial peptides have a strong killing effect on more than 40 kinds of Gram-positive and negative bacteria, Pseudomonas aeruginosa and paratyphoid bacteria and other drug-resistant strains. Antimicrobial peptides are the focus and focus of biopharmaceutical research...

AI Technical Summary

Problems solved by technology

[0005] However, there is currently no use of immune-inducing biotechnology, using the middle silk gland of the fifth-instar silkworm to synthesize high-volume sericin antimicrobial peptides, synthesizing silk fibroin antimicrobial peptides in the posterior silk gland, and then synthesize and expand through solid-phase or liquid-phase biotechnology. Increase, preparation of broad-spectrum antibiotics for agricultural antibacterial production