Method for preparing broad spectrum antibiotics through immune induction biotechnology

A technology of immune induction and biotechnology is applied in the field of preparation of antibiotics to achieve the effects of broad-spectrum antibacterial advantages, strong antibacterial effects and low production costs

Inactive Publication Date: 2017-07-04
TIANJIN GUANGMAOTAI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is currently no use of immune-inducing biotechnology, using the middle silk gland of the fifth-instar silkworm to synthesize high-volume sericin antimicrobial peptides, synthesizing silk fibroin antimicrobial peptides in the posterior silk gland, and then synthesize and expand through solid-phase or liquid-phase biotechnology. Increase, preparation of broad-spectrum antibiotics for agricultural antibacterial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 adopts method of the present invention, prepares a kind of broad-spectrum antibiotic that is used for agricultural antibacterial production according to the following steps:

[0021] (1) Antibody strain: Bacillus subtilis; Biologically active agent: Biokinesin derived from the independent intellectual property research results of Zhanjiang Normal University;

[0022] (2) Preparation steps:

[0023] ① When the natural fifth instar silkworm grows to the third day, when sericin is synthesized at 1 / 2 of the middle silk gland of the natural fifth instar silkworm body, use an inoculator to inoculate Bacillus subtilis into the 1 / 2 of the middle silk gland of the natural fifth instar silkworm body place, immune induction, the natural five-instar silkworm body reached the peak of immunity 3 days after inoculation;

[0024] ② During the immune peak period, freeze-dry the natural fifth-instar silkworm body in the immune peak period until the water content is 70%, the...

Embodiment 2

[0027] Embodiment 2 adopts method of the present invention, prepares a kind of broad-spectrum antibiotic that is used for agricultural antibacterial production according to the following steps:

[0028] (1) Antibody strain: Bacillus subtilis; Biologically active agent: Biokinesin derived from the independent intellectual property research results of Zhanjiang Normal University;

[0029] (2) Preparation steps:

[0030] ① When the natural fifth instar silkworm grows to the third day, when sericin is synthesized at 1 / 2 of the middle silk gland of the natural fifth instar silkworm body, use an inoculator to inoculate Bacillus subtilis into the 1 / 2 of the middle silk gland of the natural fifth instar silkworm body place, immune induction, 4 days after inoculation, the body of natural fifth-instar silkworm reached the peak of immunity;

[0031] ②In the peak period of immunity, the natural five-instar silkworm body in the immune peak period is directly ground and pulverized, and the...

Embodiment 3

[0034] Embodiment 3 adopts method of the present invention, prepares a kind of broad-spectrum antibiotic that is used for agricultural antibacterial production according to the following steps:

[0035](1) Target antibody strain: Bacillus subtilis; Bioactive agent: Biokinin produced from independent intellectual property research results of Zhanjiang Normal University;

[0036] (2) Preparation steps:

[0037] ① When the natural fifth instar silkworm grows to the third day, when silk fibroin is synthesized at the tail silk gland of the natural fifth instar silkworm, use an inoculator to inoculate Bacillus subtilis into the silk gland of the natural fifth instar silkworm body, induce immunity, and inoculate The natural five-instar silkworm body reaches the peak of immunity in the last 5 days;

[0038] ②During the immune peak period, freeze-dry the natural fifth-instar silkworm body in the immune peak period until the water content is 75%, then put the freeze-dried natural fifth...

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PUM

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Abstract

The invention discloses a method for preparing broad spectrum antibiotics through an immune induction biotechnology. The method for preparing broad spectrum antibiotics through the immune induction biotechnology uses the super strong immunoprotein synthesis ability of silkworms, sericin antibacterial peptides are highly synthesized at middle sericteria of natural fifth-instar silkworm larvae, silk fibroin antibacterial peptides are synthesized at rear sericteria, and the broad spectrum antibiotics are prepared by regulating fibroin gene by a biologic high-active agent and carrying out synthesis and amplification by a solid phase or liquid phase biotechnology. The method has the advantages of simple and easily learned biotechnology synthesis route, low production cost, high controllability of the yield, high disease resistance reaching 80-95% or above, easiness in achievement transformation, and convenience in industrial production; and the antibacterial peptides prepared through adopting the method are mixed with soil when used in the agriculture, requires root soaking before transplantation, and has the advantages of strong antibacterial effect and high-efficiency and broad-spectrum antibiosis in the treatment of infection caused by Gram-positive bacteria, Gram-negative bacteria or Bacillus subtilis, bacterial wilt and other pathogens, and Fusarium wilt.

Description

technical field [0001] The invention relates to a method for preparing antibiotics, in particular to a method for preparing broad-spectrum antibiotics by immune induction biotechnology. Background technique [0002] Antibacterial peptides, also known as peptide antibiotics or antimicrobial peptides, are a type of active peptides produced by the biological immune system to resist external biological infection sources and widely exist in insects, plants, animals and humans. In addition to its non-specific anti-bacteria, fungus, virus, anti-infection, and anti-disease effects, it also has anti-cell mutation and anti-tumor cells without damaging normal human cells, especially for multi-drug resistant strains. Antimicrobial peptides have a strong killing effect on more than 40 kinds of Gram-positive and negative bacteria, Pseudomonas aeruginosa and paratyphoid bacteria and other drug-resistant strains. Antimicrobial peptides are the focus and focus of biopharmaceutical research ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C05F1/00A01N63/02A01P1/00A01P3/00
CPCA01N63/10C05F1/00C12P21/02Y02A40/20
Inventor 孙景红
Owner TIANJIN GUANGMAOTAI BIOTECH CO LTD
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