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Polyacrylic ester and polyacrylic ester copolymer material hydrophilic modification products and hydrophilic modification method of polyacrylic ester

A polyacrylate, hydrophilic modification technology, applied in a method and the products obtained by the method, in the field of super-macroporous polyacrylate materials, can solve the problem of protein irreversible adsorption, Denaturation and other problems, to achieve the effect of reducing non-specific adsorption and improving hydrophilicity

Active Publication Date: 2012-08-01
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some characteristics of the polymer itself limit its application in the separation of biological macromolecules, such as polyglycidyl methacrylate and divinylbenzene copolymer (PGMA-DVB) microspheres, which have a large number of rings on the surface of the microspheres. Oxygen groups are easy to be derivatized into various functional groups, but due to the hydrophobicity of the surface, it is easy to have hydrophobic interactions with protein molecules, resulting in irreversible adsorption or even denaturation of proteins

Method used

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  • Polyacrylic ester and polyacrylic ester copolymer material hydrophilic modification products and hydrophilic modification method of polyacrylic ester
  • Polyacrylic ester and polyacrylic ester copolymer material hydrophilic modification products and hydrophilic modification method of polyacrylic ester
  • Polyacrylic ester and polyacrylic ester copolymer material hydrophilic modification products and hydrophilic modification method of polyacrylic ester

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1) Super-macroporous PGMA-DVB microspheres are pretreated in dioxane

[0040] Accurately weigh 1.0 g of PGMA-DVB microspheres into a 50 mL Erlenmeyer flask, then add 10 mL of dioxane, shake at room temperature on a 120 rpm shaking shaker for 2 hours, perform suction filtration, and use the same volume of dioxane After washing, the solvent is suction-filtered until there is no obvious solvent on the surface of the microspheres.

[0041] 2) Agarose bound to the surface of PGMA-DVB microspheres (Mw=13W)

[0042] Weigh 1.0g of agarose (Mw=13W) and 0.32g of NaOH into a 100mL three-neck flask, then add 50mL of DMSO / H2O (1:5; v / v) mixed solvent to it, stir mechanically at 120rpm, and add the Step 1) Swell the treated microspheres, raise the temperature to 70°C, and keep the reaction at this temperature for 24 hours. After the reaction is hot, use a G4 sand core funnel to carry out vacuum filtration, and at the same time use 500mL of 80°C hot water to filter washing.

[0043...

Embodiment 2

[0046] 1) Pretreatment of ultra-large pore PGMA-DVB microspheres in DMSO

[0047] Accurately weigh 1.0 g of PGMA-DVB microspheres into a 50 mL Erlenmeyer flask, then add 20 mL of DMSO, shake at room temperature on a 120 rpm shaking shaker for 2 hours, perform suction filtration, and wash with the same volume of DMSO. After washing, the solvent Suction filtration until there is no obvious solvent on the surface of the microspheres.

[0048] 2) Dextran bound to the surface of PGMA-DVB microspheres (Mw=2W)

[0049] Weigh 1.0g dextran (Mw=2W), 0.32g NaOH into a 100mL three-neck flask, and then add 50mL DMSO / H 2 O (1:5, v / v) mixed solvent, under mechanical stirring at 120rpm, add the microspheres after swelling treatment in step 1) in Example 2, heat up to 37°C, and keep the reaction at this temperature for 20h. After the reaction, Suction filtration under reduced pressure with a G4 sand core funnel while hot, and at the same time wash with 500 mL of 40°C deionized water.

[005...

Embodiment 3

[0053] 1) Pretreatment of ultra-large pore PGMA-DVB microspheres in DMF

[0054] Accurately weigh 1.0 g of PGMA-DVB microspheres into a 50 mL Erlenmeyer flask, then add 15 mL of DMF, shake at room temperature on a 120 rpm shaking shaker for 2 hours, perform suction filtration, and wash with the same volume of DMF. After washing, the solvent Suction filtration until there is no obvious solvent on the surface of the microspheres.

[0055] 2) Dextran bound to the surface of PGMA-DVB microspheres (Mw=5W)

[0056] Weigh 1.0g dextran (Mw=5W), 0.32g NaOH and put it into a 100mL three-necked flask, then add 50mL DMSO / H2O (1:5, v / v) mixed solvent therein, under 120rpm mechanical stirring, add the Step 1) Swell the microspheres in Example 3, raise the temperature to 47°C, and keep the reaction at this temperature for 24 hours. Perform a wash.

[0057] 3) Reinforcing PGMA-DVB microsphere surface-bound dextran with EDGE cross-linking (Mw=5W)

[0058] The microspheres prepared in step ...

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Abstract

The invention discloses a method for hydrophilically modifying a polyacrylic ester or polyacrylic ester copolymer material and a product prepared with the method. The modification method comprises the following steps of: (1) performing swelling pretreatment on microspheres by using an organic solvent; (2) under the action of an alkaline catalyst, catalyzing ring opening of an epoxy group for reacting with hydroxy groups of polysaccharides to generate a stable ester bond, and chemically bonding hydrophilic polysaccharide molecules on the surfaces of the microspheres; and (3) under an alkaline condition, crosslinking and reinforcing the polysaccharide molecules bonded on the surfaces of the microspheres by using a bifunctional crosslinking agent. The method provided by the invention is easy and convenient to operate, and has mild reaction conditions; and an obtained hydrophilic material is stable, a coating is prevented from falling easily, and nonspecific adsorption of polymer microspheres on proteins is well suppressed. According to hydrophilically-modified polyacrylic ester microspheres, rich hydroxy groups contained in a hydrophilic layer can be further derived into other various functional groups; and the microspheres have great application prospects in the field of biotechnology, particularly in the field of biochemical separation.

Description

technical field [0001] The invention relates to the field of modification of polymer materials, in particular to a method for making the surface of polymer materials hydrophilic and products obtained by the method, especially for making the surface of super-macroporous polyacrylate materials hydrophilic. Background technique [0002] As a biochemical separation medium, polymer microspheres have the advantages of high mechanical strength, stable chemical properties, good acid and alkali resistance, and can be operated under high pressure, so they have a good prospect in the field of application as a chromatographic medium. However, some characteristics of the polymer itself limit its application in the separation of biological macromolecules, such as polyglycidyl methacrylate and divinylbenzene copolymer (PGMA-DVB) microspheres, which have a large number of rings on the surface of the microspheres. Oxygen groups are easy to derivate into various functional groups, but because...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08J7/12C08J7/02C08L33/14C08L25/16C08L5/02C08L5/12
Inventor 马光辉张荣月李娟周炜清苏志国
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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