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Molecular marker for wheat stripe rust disease-resistant new gene Yr50 assisted selection and using method thereof

A wheat stripe rust and molecular marker technology, applied in the field of plant molecular genetics and wheat germplasm innovation, can solve the problems of complex RFLP operation, narrow genetic basis of wheat, limited screening and application, etc., to reduce costs, improve selection efficiency, high cost effect

Inactive Publication Date: 2012-08-01
CROP SCI RES INST SHANXI ACADEMY OF AGRI SCI
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Problems solved by technology

However, due to the relatively narrow genetic basis of wheat, in the commonly used molecular marker system, the RFLP operation is complex and the polymorphism is low, which limits the screening and application of wheat RFLP markers to a certain extent.

Method used

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  • Molecular marker for wheat stripe rust disease-resistant new gene Yr50 assisted selection and using method thereof
  • Molecular marker for wheat stripe rust disease-resistant new gene Yr50 assisted selection and using method thereof
  • Molecular marker for wheat stripe rust disease-resistant new gene Yr50 assisted selection and using method thereof

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Embodiment Construction

[0035] Tritium intermedia contains many beneficial genes that improve wheat quality and enhance wheat resistance, such as the currently officially named powdery mildew resistance gene, Pm40 (Luo et al. Theoretical and Applied Genetics, 2009, 118:1059-1064) and Pm43 (He et al. Theoretical and Applied Genetics, 2009, 118:1173-1180) are all from Thiopyrum intermedia. Through the inoculation identification of 29, 31, 32 and 33 in the current popular stripe rust races, it was found that E. intermedia, octoploid E. tritifolius-TAI7047 and those from "Jinmai 33 / TAI7047∥2*Jing 411" BC 2 f 6 The CH223 strain bred from the progeny materials was immune to the four races (Table 2), indicating that Thiopyrum intermedium contains excellent stripe rust resistance genes. In order to better utilize the stripe rust resistance gene in Thiopyrum intermedia to improve wheat, we mapped the stripe rust resistance gene in CH223 and developed molecular markers to realize marker-assisted selection ...

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Abstract

The invention discloses molecular marker for wheat stripe rust disease-resistant new gene Yr50 assisted selection and a using method thereof, belongs to the technical field of plant molecular genetics and wheat germplasm innovation and relates to a molecular marker for a wheat stripe rust disease-resistant new gene and a positioning method of the molecular marker. The molecular marker for wheat stripe rust disease-resistant new gene Yr50 assisted selection consists of a marker Xbarc1096 and a marker Xwmc310, wherein the genetic distance between the marker Xbarc1096 and a wheat stripe rust resistance gene Yr50 is 6.9cM and the genetic distance between the marker Xwmc310 and the wheat stripe rust resistance gene Yr50 is 7.2cM. The wheat stripe rust disease-resistant new gene Yr50 which is originated from Thinopyrum is discovered for the first time in the world and the molecular marker which is linked with the wheat stripe rust disease-resistant new gene Yr50 is obtained. By using the wheat stripe rust molecular marker assisted breeding method provided by the invention, the selected targets are clear and the cost can be saved. Not only can a new resistant resource be provided for wheat stripe rust disease-resistant breeding, but also material and technical foundations are laid for map-based cloning of the gene.

Description

technical field [0001] The invention belongs to the field of plant molecular genetics and wheat germplasm innovation technology, and relates to the molecular marker development and positioning method of a new gene for resistance to wheat stripe rust, in particular to a new gene for resistance to wheat stripe rust Yr50 Molecular markers for assisted selection and their use. Background technique [0002] by stripe rust ( P. striiformis f. sp. tritici ) caused by wheat stripe rust is an important wheat disease worldwide, and my country is the endemic area of ​​the disease, and its incidence area and disaster frequency rank first among wheat diseases. Since the 1950s, there have been 15 moderate epidemics and 4 major epidemics in my country. The most recent large-scale epidemic occurred in 2002. Due to the emergence and prevalence of new toxic races such as Tiaozhong No. 31 (CYR31) and Tiaozhong No. 32 (CYR32), the elimination of Yr5 , Yr10 , Yr15 , Yr24 and Yr26 Most s...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 畅志坚张晓军杨足君贾举庆李欣詹海仙张丛卓
Owner CROP SCI RES INST SHANXI ACADEMY OF AGRI SCI
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