Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Polymerase chain reaction (PCR) primers, method and kit for identifying duck gender

A kit and gender technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as low accuracy, deep cloaca, difficult to open, etc., and achieve accurate results. , The effect of quick identification, simple and convenient operation

Inactive Publication Date: 2012-08-01
JIANGSU INST OF POULTRY SCI
View PDF2 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the appearance identification method and the syrinx identification method are simple and convenient, the accuracy rate is low, and identification errors are prone to occur; while the anal identification method and anal identification method must be operated within 12 hours after ducks emerge from the shell. The characteristic of chick genital bulge is the most obvious. More than 24 hours after hatching, the anus is tight and difficult to open, and the genital process shrinks, and even sinks into the deep part of the cloaca, which is inconvenient to observe

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polymerase chain reaction (PCR) primers, method and kit for identifying duck gender
  • Polymerase chain reaction (PCR) primers, method and kit for identifying duck gender
  • Polymerase chain reaction (PCR) primers, method and kit for identifying duck gender

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A pair of PCR primers PF (SEQ ID NO.1) and PR (SEQ ID NO.2) are designed using the CHD1 gene sequence present on duck sex chromosome W chromosome and Z chromosome; the sequence of the primers is:

[0034] Upstream primer PF: 5'-AGTGCATTGCAGAAGCAATATT-3';

[0035] Downstream primer PR: 5'-GCCTCCTGTTTATTATAGAATTCAT-3'.

Embodiment 2

[0038] Step 1. After the anticoagulated blood of 102 Gaoyou ducks was routinely lysed and digested, the total DNA was extracted by the traditional phenol / chloroform method, and the total DNA concentration was diluted to 50ng / μL as template DNA;

[0039] Step 2, using a kit containing PCR primers PF (SEQ ID NO.1) and PR (SEQ ID NO.2) to carry out PCR amplification of the CHD1 gene in the template DNA described in step 1, the PCR amplification reaction system ( 25μL system) is: template DNA 45ng~55ng, 2×PCR amplification buffer 12.5μL, 5μmol / L upstream primer 2μL, 5μmol / L downstream primer 2μL, add water to 25μL; PCR amplification reaction conditions are: 94 ℃ pre-denaturation 5min, then denaturation at 94°C for 30s, renaturation at 55°C for 30s, extension at 72°C for 30s, 35 cycles, and finally extension at 72°C for 7min;

[0040] Step 3: Take 5 μL of the PCR amplification product obtained in Step 2, and use 2% agarose gel electrophoresis to identify the PCR amplification produ...

Embodiment 3

[0044] Step 1. After the anticoagulated blood of 8 mountain shelducks, 8 Xingyi ducks, 8 Guangxi shelducks and 8 Yunnan shelducks were conventionally lysed and digested, the total blood was extracted by traditional phenol / chloroform method. DNA, and dilute the total DNA concentration to 50ng / μL as template DNA;

[0045]Step 2, using a kit containing PCR primers PF (SEQ ID NO.1) and PR (SEQ ID NO.2) to carry out PCR amplification of the CHD1 gene in the template DNA described in step 1, the PCR amplification reaction system ( 25μL system) is: template DNA 45ng~55ng, 2×PCR amplification buffer 12.5μL, 5μmol / L upstream primer 2μL, 5μmol / L downstream primer 2μL, add water to 25μL; PCR amplification reaction conditions are: 94 ℃ pre-denaturation 5min, then denaturation at 94°C for 30s, renaturation at 55°C for 30s, extension at 72°C for 30s, 35 cycles, and finally extension at 72°C for 7min;

[0046] Step 3. Take 5 μL of the PCR amplification product obtained in step 2, and use 2%...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses polymerase chain reaction (PCR) primers for identifying duck gender. The nucleotide sequences of the primers are shown as SEQ ID NO. 1 and SEQ ID NO.2 in a sequence table. In addition, the invention also provides a method for identifying the duck gender by using the PCR primers for identifying the duck gender and a kit containing the PCR primers for identifying the duck gender. The method comprises the following steps of: performing PCR amplification by using total DNA of a duck to be detected as a template and adopting the PCR primers for identifying the duck gender, detecting the PCR amplification product by adopting agarose gel electrophoresis, and judging the duck gender. The PCR primers are reasonably designed by using a CHD1 gene sequence concurrent in a duck sex chromosome W and a chromosome Z, and the gender of the individual duck is determined by combining the PCR amplification and the agarose gel electrophoresis; and the method is easy and convenient to operate, has the advantages of quick identification, accurate result and the like, and overcomes the defects that the conventional method wastes time and labor and easily produces errors.

Description

technical field [0001] The invention belongs to the technical field of biological detection and identification, and in particular relates to a PCR primer for duck sex identification, an identification method and a kit. Background technique [0002] At present, the identification methods of duck sex are generally appearance identification method, syrinx identification method, anal identification method and anal turning identification method. Although the appearance identification method and the syrinx identification method are simple and convenient, the accuracy rate is low, and identification errors are prone to occur; while the anal identification method and anal identification method must be operated within 12 hours after ducks emerge from the shell. The characteristic of chick genital bulge is the most obvious. More than 24 hours after hatching, the anus is tight and difficult to open, and the genital process shrinks, and even sinks into the deep part of the cloaca, which...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 李慧芳胡艳宋迟束婧婷单艳菊朱文奇宋卫涛汤青萍朱春红陶志云章双杰韩威章明
Owner JIANGSU INST OF POULTRY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com