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Artificial cultivation method for cordyceps militaris mycelium for increasing cordycepin content

A technology of Cordyceps militaris mycelium and artificial cultivation, applied in the field of bioengineering, can solve the problems of no reports, etc., and achieve the effect of increasing yield and high yield of cordycepin

Active Publication Date: 2013-07-24
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there is no report on the artificial cultivation method of increasing the yield of cordycepin through manganese ion stress at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Strain: Cordyceps militaris strain 5.270, from Guangdong Microbial Strain Collection Center.

[0019] Test tube slant culture (activation) (can also be cultivated according to other conventional methods):

[0020] Preparation medium: glucose 10-60g, peptone 1-10g, yeast extract 1-10g, magnesium sulfate 1-5g, potassium dihydrogen phosphate 1-5g, agar powder 15-20g, water 890-971g, and its pH value is 5.0-7.0, divided into test tubes; put Cordyceps militaris strains into the slant of test tubes under sterile conditions, and cultivate in dark at 15-30°C for 5-10 days;

[0021] Test tube liquid culture (can also be cultivated according to other conventional methods):

[0022] Preparation medium: glucose 10-60g, peptone 1-10g, yeast extract 1-10g, magnesium sulfate 1-5g, potassium dihydrogen phosphate 1-5g, water 910-986g, its pH value is 5.0-7.0, pack in aliquots In a test tube, sterilize; insert the activated slant bacteria, 15~30℃, stirring speed 120rpm, culture for 5 d...

Embodiment 2

[0030] Strain: Cordyceps militaris strain Cordyceps militaris 5.701, which comes from China Common Microorganism Culture Collection and Management Center.

[0031] Test tube slant culture (activation) (can also be cultivated according to other conventional methods):

[0032] Preparation medium: glucose 10-60g, peptone 1-10g, yeast extract 1-10g, magnesium sulfate 1-5g, potassium dihydrogen phosphate 1-5g, agar powder 15-20g, water 890-971g, and its pH value is 5.0-7.0, divided into test tubes; put Cordyceps militaris strains into the slant of test tubes under sterile conditions, and cultivate in dark at 15-30°C for 5-10 days;

[0033] Test tube liquid culture (can also be cultivated according to other conventional methods):

[0034] Preparation medium: glucose 10-60g, peptone 1-10g, yeast extract 1-10g, magnesium sulfate 1-5g, potassium dihydrogen phosphate 1-5g, water 910-986g, its pH value is 5.0-7.0, pack in aliquots In a test tube, sterilize; insert the activated slant b...

Embodiment 3

[0042] Strain: Cordyceps militaris strain 5.270, from Guangdong Microbial Strain Collection Center.

[0043] Test tube slant culture (activation) (can also be cultivated according to other conventional methods):

[0044] Preparation medium: glucose 20g, peptone 6g, yeast extract 5g, magnesium sulfate 1g, potassium dihydrogen phosphate 1g, agar powder 15g, water 952g, its pH value is 5.0-7.0, and it is divided into test tubes; Cordyceps militaris Insert the species into the slant of the test tube under sterile conditions, and culture in the dark at 15-30°C for 5-10 days;

[0045] Test tube liquid culture (can also be cultivated according to other conventional methods):

[0046] Preparation medium: glucose 10-60g, peptone 1-10g, yeast extract 1-10g, magnesium sulfate 1-5g, potassium dihydrogen phosphate 1-5g, water 910-986g, its pH value is 5.0-7.0, pack in aliquots In a test tube, sterilize; insert the activated slant bacteria, 15~30℃, stirring speed 120rpm, culture for 5 day...

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PUM

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Abstract

The invention discloses an artificial cultivation method for cordyceps militaris mycelium for increasing cordycepin content. The artificial cultivation method comprises the steps of test tube slant strain cultivation, test tube liquid strain cultivation, triangular flask liquid seed cultivation, seed jar cultivation and fermentation tank cultivation. The artificial cultivation method is characterized in that: during the step of fermentation tank cultivation, when the yield of mycelium is 0.4-0.8% from the seed jar cultivation, the fermentation tank cultivation is started; 1%-9% of manganese sulfate by weight is contained in a culture medium; the temperature is at 23-26 DEG C; aerating is at a ratio of 1:0.5; the stirring speed is 120rpm-150rpm; and when the yield of the mycelium is 1.2-1.8% and the content of raw sugar in the culture medium is at most 0.2%, the jar is released and the cordyceps militaris mycelium is harvested. According to the artificial cultivation method disclosed by the invention, under the stressing action of manganese ions, the cordycepin content in the cordyceps militaris mycelium is increased by above 0.8 times than that in a reference culture medium without manganese sulfate, an effective method for increasing the yield is supplied to the production of the cordycepin products, and the market prospect is wide.

Description

Technical field: [0001] The invention relates to an artificial cultivation method of Cordyceps militaris mycelium which increases the yield of cordycepin through manganese ion stress, and belongs to the technical field of bioengineering. Background technique: [0002] Cordycepin (3'-deoxyadenosine) is a characteristic component of some fungi of the genus Cordyceps, and it is also one of the most important active ingredients in fungi of the genus Cordyceps. [0003] Cordycepin has functions such as immune regulation, anti-tumor, anti-fatigue, and cardiopulmonary regulation. As a new type of broad-spectrum antibiotic, cordycepin can inhibit the RNA synthesis of viruses with its unique antibacterial and antiviral activity; it has inhibitory effects on Bacillus subtilis and Mycobacterium avium; it also has a killing effect on HIV-I virus; In particular, it has a strong inhibitory effect on a variety of solid malignant tumors. Cordycepin shows excellent pharmaceutical applicati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04
Inventor 程显好左言美刘静刘林德李维焕
Owner LUDONG UNIVERSITY