Acinetobacter with authigenic nitrogen fixation capacity and application thereof

An ability and nitrogen fixation technology, applied in the field of Acinetobacter, can solve the problems of low resistance to heavy metal growth and nitrogen fixation, slow growth, and can not meet the remediation of polluted soil.

Inactive Publication Date: 2012-08-15
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most wild nitrogen-fixing bacteria have the disadvantages of slow growth, heavy metal resis

Method used

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  • Acinetobacter with authigenic nitrogen fixation capacity and application thereof
  • Acinetobacter with authigenic nitrogen fixation capacity and application thereof
  • Acinetobacter with authigenic nitrogen fixation capacity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0060] Example 1

[0061] According to the method for screening nitrogen-fixing strains provided by the present invention, nitrogen-fixing strains with high nitrogenase activity that can tolerate heavy metal cadmium are selected, and the preparation steps are as follows:

[0062] 1) Take the autogenous azotobacter Acinetobacter YSGD07 selected from the rhizosphere of the plant as the starting strain;

[0063] 2) Mutagenesis and breeding

[0064] (1) Prepare a single cell suspension of the starting strain YSGD07

[0065] The starting strain YSGD07 was inoculated into liquid medium A, cultured at 28°C, 150rpm for 16hrs, centrifuged, washed with sterile saline, placed in an Erlenmeyer flask filled with glass beads, shaken to disperse it into a single cell suspension liquid;

[0066] The composition of the medium A is: yeast powder 10g, NaCl 5g, peptone 10g, FeSO 4 ·7H 2 O 0.005g, Na 2 MoO 4 ·2H 2 O 0.0025g, distilled water 1000ml, pH 7.0~7.2.

[0067] (2) UV mutagenesis

[0068] Adjust the...

Example Embodiment

[0077] Example 2

[0078] According to the method for preparing nitrogen-fixing bacteria provided by the present invention, the nitrogen-fixing bacteria with high nitrogenase activity that can tolerate heavy metal cadmium is prepared, and the preparation steps are as follows:

[0079] The preparation of the bacterial agent includes the following steps:

[0080] 1. Strain fermentation

[0081] ①First level flask liquid culture

[0082] Quickly thaw the frozen Ymu7-3 at 37°C, insert 0.5% of the inoculum into an Erlenmeyer flask containing liquid medium A, culture at 28°C, 150rpm with shaking for 18hrs;

[0083] The composition of the liquid medium A is: the composition of the medium A is: yeast powder 10g, NaCl 5g, peptone 10g, FeSO 4 ·7H 2 O 0.005g, Na 2 MoO 4 ·2H 2 O 0.0025g, distilled water 1000ml, pH 7.0~7.2

[0084] ②Secondary fermentation tank fermentation

[0085] 1) Connect the primary culture solution of Ymu7-3 to a fermentor equipped with liquid medium E at a 5% inoculum for ferm...

Example Embodiment

[0089] Example 3

[0090] Using the bacterial agent of Example 2 to remediate cadmium-contaminated soil with intensified Indian mustard, the specific steps are:

[0091] The potting soil was collected from farmland soil in Chang'an County, Shaanxi Province. The total Cd content of the tested soil was 0.25 mg / kg. The soil was set with 4 Cd dosings. 2+ The levels are 0, 20, 40, 80 mg / kg, and the corresponding amount of CdCl 2 Prepare the solution so that the test soil and heavy metals are repeatedly mixed uniformly, and left to stand for 30 days. The experiment set up a blank control group, a urea addition group and a bacteria addition group. After emergence, 3 strains are retained in each pot. Dilute the above-prepared liquid inoculum at a ratio of 1:100. Use root irrigation to pour 50ml of diluted bacteria in each pot. Pour every 3 weeks without adding bacteria. The same dose of dead bacteria was poured in the group, and the urea concentration in the urea group was 0.12g / L. The...

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Abstract

The invention discloses an acinetobacter with authigenic nitrogen fixation capacity, which is classified and named as Ymu7-3 and is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of CGMCC No. 5006. The free living nitrogen fixing bacteria has high nitrogen fixing capacity, is capable of resisting the growth of Cd with higher concentration, and can secrete heteroauxin so as to generate iron atom; and the prepared microbial bacterium agent can remarkably promote the growth of hyper-accumulating plants, increase the biomass liveweight of the hyper-accumulating plants, greatly improve the heavy metal enrichment factor and transfer coefficient of the hyper-accumulating plants, and improve the ecological environment of the soil, thus being environment-friendly and low in cost.

Description

technical field [0001] The invention relates to a strain of Acinetobacter with self-generated nitrogen-fixing ability. The Acinetobacter is obtained by ultraviolet-plasma compound mutagenesis, has the ability to resist the growth of heavy metals, can be used for environmental treatment of soil heavy metal pollution, and belongs to the field of microbial application technology. Background technique [0002] Heavy metal pollution refers to environmental pollution caused by heavy metals or their compounds, mainly caused by human factors such as mining, waste gas discharge, and sewage irrigation. In recent years, with the growth of population, the rapid development of industry, and the extensive use of chemical fertilizers and pesticides, soil heavy metal pollution has become increasingly serious. [0003] Cd is a highly toxic heavy metal. The Japanese "Itai-Itai Disease" that shocked the world was caused by cadmium pollution. Cadmium will replace calcium in bone, softening and...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C05F11/08C12R1/01
Inventor 朱晓丽马沛范代娣申烨华梁丽华
Owner NORTHWEST UNIV
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