8 results about "Azotobacter sp." patented technology

According to the high-temperature-resistant nitrogen-fixing bacteria microcapsule fertilizer and the preparation method thereof, aiming at the problems of thallus inactivation and nutrient loss in a high-temperature environment, a sodium alginate-chitosan double-layer embedding technology is combined with magnetic nanoparticle modification, and a stable double-layer structure is formed by optimizing the ratio of an embedding material, so that the high-temperature-resistant nitrogen-fixing bacteria microcapsule fertilizer is prepared. The survival rate and activity of the nitrogen-fixing bacteria at high temperature are remarkably improved, and meanwhile, the functions of intelligent nutrient release and magnetic recovery are achieved. The fertilizer is prepared from composite microcapsules, amino acid, humic acid and other raw materials according to a specific ratio through magnetic field orientation treatment, multi-stage mixing, atomization granulation and magnetic field screening processes, the particle size is controlled to be 0.5-3.35 mm, and the shelf life is prolonged to 12 months. The method has the technical effects that trehalose-glycerol / sorbitol synergistically forms a glassy protective film to maintain the stability of thalli, gamma-aminobutyric acid activates nitrogenase gene expression, magnetic nanoparticles construct a photosynthetic symbiotic system to optimize rhizosphere oxygen supply, and silanized FeO nanoparticles fix heavy metal ions through electrostatic adsorption, so that the heavy metal ions in the thalli are effectively adsorbed, and the heavy metal ions in the thalli are effectively adsorbed. And the humic acid buffers the pH fluctuation of the soil, so that the nitrogen fixation efficiency is finally improved by 30-50%, and the crop yield is increased by 15-25%.

A phage and its use in compensating soil nitrogen fixation: Klebsiella pneumoniae phage φYSZKA, wherein the method has solved some problems of conventional azotobacters, such as high cost, interspecific competition and limited range of application. Through phage transplantation, the efficiency of microbial nitrogen fixation in soil can be improved, and thus, more nitrogen sources may be provided for the nitrogen cycle and plant growth in ecosystems. Also, a new solution is provided for the nitrogen sequestration process of soil in saline-alkali lands, tea plantations and pesticide-polluted sites in China.

The application relates to a nitrogen-fixing spirillum (Azospirillum sp.) I102 with a preservation number of CGMCC No. 34685. Azospirillum The strain is a new species of the Azospirillum genus, can produce indole-3-acetic acid at a high yield, and can grow under the conditions of pH 5-9, NaCl concentration 0-4%, and temperature 20-42 DEG C. The strain has the abilities of nitrogen fixation, iron carrier production and urease production, and can resist heavy metals such as cadmium, copper and lead. The application also relates to application of the strain in promoting seed germination and plant growth, and the strain has a good application prospect in soil improvement, development of new biological organic fertilizer and the like.

The invention belongs to the technical field of microbial agents, and particularly relates to a charcoal-based nitrogen-fixing bacterium agent as well as a preparation method and application thereof. According to the invention, a biochar-humic acid-sodium alginate embedding mode is adopted, so that a porous protection cabin can be provided to adsorb plant rhizosphere nutrients. Meanwhile, a low-oxygen microcell is created to protect nitrogenase, physical protection is provided for thalli, and external environmental stress is buffered. Wherein humic acid is used as a high-quality organic carbon source and is beneficial to stimulating thalli to rapidly proliferate in embedded particles and rhizosphere, and a large number of active cells can be obtained in a short time. The biochar-based nitrogen-fixing bacterium agent (namely biochar-humic acid-sodium alginate microspheres) prepared by the invention has the advantages of simple and convenient preparation method, stable storage performance and the like, and compared with other biological agents, the biomass of the dried microbial agent reaches 2.2 * 10 < 10 > CFU / g, so that the biochar-based nitrogen-fixing bacterium agent has a good application prospect.

The invention discloses azospirillum sp. Sgz302134 and application thereof, the taxonomic name of the azospirillum sp. Sgz302134 is Azospirillum sp., the azospirillum sp. Sgz302134 is preserved in Guangdong Microbial Culture Collection Center on August 11, 2025, and the preservation number of the azospirillum sp. Sgz302134 is GDMCC No: 66829. The azospirillum sp. Sgz302134 disclosed by the invention has an azotobacter function and can be used for reducing nitrogen into nitrogen fertilizer which can be utilized by crops, so that the azospirillum sp. Sgz302134 can be further driven to be applied to rice growth promotion and the like in agricultural production, application of chemical nitrogen fertilizer is reduced, environmental pollution is reduced, and the azospirillum sp. Sgz302134 has a good application prospect.

The invention provides a preparation method of a microbial flocculant for efficiently removing antibiotics, which comprises the following steps: inoculating a Vilnelande azotobacter AZ6 strain into fermentation liquor, carrying out shake culture, and mixing the obtained fermentation liquor with bacterial liquid with glycerol to obtain preserved bacterial liquid; inoculating the preserved bacteria solution into a new fermentation solution, carrying out shake culture, centrifuging, and collecting a supernatant; adding absolute ethyl alcohol into the supernate, uniformly mixing, standing, centrifuging and collecting precipitate; and freeze-drying the precipitate to obtain the microbial flocculant. The invention also provides application of the microbial flocculant for removing antibiotics in water. The microbial flocculant is used for removing antibiotics in water. The microbial flocculant disclosed by the invention is applied to a mechanism of removing antibiotics through a synergistic effect of surface adsorption, charge neutralization, adsorption bridging and sweeping and netting.

A microbial compound inoculant, based on Klebsiella pneumoniae (Klebsiella pneumoniae) Klebsiella sp. CS11, long-branch mold ( Trichoderma longibrachiatum ) and Azotobacter brownii ( Azotobacter chroococcum The strain, Klebsiella pneumoniae CS11, was composed of bacteria in a mass ratio of 1:1:1 and its preservation number is CCTCC M 20242943. CS11 When applied to aerobic composting of corn stalks and chicken manure, the strain can reduce H2S emissions and improve sulfur metabolism efficiency during composting. Furthermore, the compound microbial agent in this invention further accelerates the decomposition and transformation of organic matter, reduces H2S emissions, and improves sulfur fixation efficiency. Simultaneously, it regulates the content of amino acids and humic acid in the compost, producing high-quality organic fertilizer, improving the soil environment, achieving resource recycling, and promoting sustainable agricultural development.

The invention provides a soil biological conditioning agent for rapidly increasing the earthworm proliferation rate. The soil biological conditioning agent is prepared from a compound microbial agent, a functional enzyme preparation and a humic acid carrier containing calcium alginate, the compound microbial agent comprises bacillus amyloliquefaciens (Bacillus amyloliquefaciens), azotobacter chroococcum (Azotobacter chroococcum) and Saccharomyces cerevisiae (Saccharomyces cerevisiae), and the compound microbial agent is prepared from bacillus amyloliquefaciens (Bacillus amyloliquefaciens), azotobacter chroococcum (Azotobacter chroococcum) and Saccharomyces cerevisiae (Saccharomyces cerevisiae (Saccharomyces cerevisiae). The functional enzyme preparation comprises cellulase and chitinase. According to the conditioning agent disclosed by the invention, a ternary synergistic system consisting of the compound microbial agent, the functional enzyme preparation and the humic acid carrier containing calcium alginate is constructed, so that a natural soil ecological process can be simulated and enhanced, and whole-course efficient regulation and control on earthworm nutrition supply and environment improvement are realized.