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59 results about "Azotobacter sp." patented technology

Alga microorganism fertilizer agent and preparation method thereof

The invention relates to an alga microorganism fertilizer agent and a preparation method thereof and belongs to the technical field of aquiculture and the like. The alga microorganism fertilizer agent is fermented in such a way that monosodium glutamate waste liquid, alga paste, photosynthetic bacteria, actinomycetes, azotobacters and bacillus subtilis are compounded, and meanwhile, chitosan, chitosan oligosaccharide, microelements and molasses are added according to appropriate parts. The alga microorganism fertilizer agent is formed in such a way that the monosodium glutamate waste liquid is adopted for fermentation, and the raw materials preparation is simple; high-content compound bacteria are contained (the content of the bacillus subtilis is larger than three hundred million / mL, and the total content of the photosynthetic bacteria, the actinomycetes and the azotobacters is larger than one hundred million / mL) and the fertilizing effect is obvious; and the alga microorganism fertilizer agent also has a function of purifying water body, has a wide application range, and is suitable for sea-water and fresh-water cultivation.
Owner:日照巴斯德生物科技有限公司

Method for producing dendrobium officinale tissue culture mycorrhizal seedlings

The invention relates to a method for producing dendrobium officinale tissue culture mycorrhizal seedlings, which comprises the following steps of: (1) culturing mycorrhizal fungi by using a solid or liquid culture medium, wherein the mycorrhizal fungi are mycorrhizal fungi LP1, namely Chaetomium sp.; (2) culturing mycorrhizal bacteria, wherein the mycorrhizal bacteria DP6 are Azotobacter sp.; (3) culturing the dendrobium officinale tissue culture seedlings; and (4) carrying out water culturing and training on the tissue culture seedlings. With the method for producing the dendrobium officinale tissue culture mycorrhizal seedlings, provided by the invention, not only the growth of the dendrobium officinale tissue culture seedlings is promoted, but also the tissue culture seedlings are obviously thickened. After transplantation, the survival rate can reach more than 95%, and the tissue culture seedlings have high growth rate, great bud tillering rate, rapid growth rate and strong stress resistance. Compared with the control group of dendrobium officinale seedlings which are not inoculated to mycorrhizal fungi and bacteria to perform tissue culture, the dendrobium officinale seedlings inoculated to mycorrhizal fungi and bacteria have more prosperous root systems, obviously inflated plant internodes and high fresh weight increased by 10-20%.
Owner:梁经军

Liquid-state composite bacterial microbe fertilizer and its preparing process

A liquid-state compound microbial fertilizer is prepared from azotobacteria, bacillus megaterium, silicate bacteria and trace elements through physical and chemical induced mutation, mixing and diluting by water. Its advantages are high yield and quality of agricultural crops, and long quality guarantee period.
Owner:福建省尤溪县绿地生物制品有限公司

Agricultural composite microbial preparation and preparing method thereof

The invention discloses an agricultural composite microbial preparation, respective zymocyte solutions of soja bean legume bacteria, round brown azotobacteria, bacillus megaterium and colloidal substance bacillus are respectively adsorbed on the adsorbing agent according to the proportionality of 15-20 % by weight to prepare soja bean legume bacteria, round brown azotobacteria, bacillus megaterium and colloidal substance bacillus, which are compounded to the agricultural composite microbial preparation; the invention adopts the composition of four kinds of bacterial seeds, also considers the requirement ofnitrogen, phosphor and kalium nutrients for plant, it is proved by the field experiment, the obvious yield increasing and quality raising actions for multiple corps are provided; the method accelerates the growth respectively adopting the controls of air flow of microorganism, temperature and inoculating amount during fermentation, enables the fermentation periods of the four kinds of composite bacterial series to be shortened, thereby fermenting at the same time and the terminating at the same time.
Owner:嵇书琼

Endophytic azotobacter of wheat producing ACC (1-aminocyclopropane-1-carboxylate) deaminase and application thereof

The invention discloses endophytic azotobacter of wheat producing ACC (1-aminocyclopropane-1-carboxylate) deaminase and application thereof. The endophytic azotobacter of wheat is pseudomonas sp. GDXM114, and is collected in the China General Microbiological Culture Collection Center (CGMCC) with the collection number of CGMCC No.5040. The pseudomonas sp. GDXM114 with the collection number of CGMCC No.5040 has the advantages of higher nitrogenase activity, ACC deaminase activity and wide application prospect in producing nitrogenase microorganism inoculants and microbial organic fertilizers.
Owner:INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI

Mix fermentation production of soil renovation bacterium agent with multiple bacterials and production method thereof

The invention discloses a soil remediation microbial inoculum produced by the fermentation of multiple-thalli mixed liquor and a preparation method thereof. Original strains of azotobacter, P- releasing bacteria, silicate bacteria, biological control bacteria, microzyme, trichoderma, penicillium, lactobacillus and photosynthetic bacteria are selected and mixed with water to form original bacteria solution which is then added into a seeding tank in a proportion of 4 percent to form seed solution. The seed solution is then added into a production tank according to a proportion of 10 percent to form the soil remediation microbial inoculum. The method includes the steps: the azotobacter, the P- releasing bacteria and the silicate bacteria are mixed with water and added into a triangular flask at the temperature controlled at 37 DEG C and cultured for 24 hours in a shaking manner; the biological control bacteria, the microzyme, the trichoderma and the penicillium are mixed with water and cultured for 48 hours to 72 hours in a swing bed and aerobic fermentation is carried out for 16 hours; the lactobacillus is mixed with water and added into a Kolle flask and cultured at the constant temperature of 37 DEG C for 24 hours; and the photosynthetic bacteria are mixed with water, added into a Kolle flask and cultured under a 60W to 80W incandescent lamp for 3 days to 5 days. The microbial inoculum and the preparation method have the advantages of promoting the reproduction of soil microorganisms, carrying out virtuous cycle, improving soil and having simple production technology and low cost.
Owner:河南省龙腾高科实业有限公司

Brevibacterium halotolerans and application thereof in ecological restoration of soils

The invention discloses a brevibacterium halotolerans with relatively high azotase activity and application thereof in ecological restoration. The brevibacterium halotolerans GDSD21 is collected in the China General Microbiological Culture Collection Center with the collection number of CGMCC No.12402. Nitrogen-fixing bacteria are separated from rhizosphere soils of field-crop roots, further strains with relatively high azotase activity are screened, and finally the efficient nitrogen-fixing bacterium brevibacterium halotolerans GDSD21 is screened. The azotase activity of the brevibacterium halotolerans GDSD21 is obviously higher than that of common production strain azotobacter chroococcum ACCC11103 for microbial fertilizers, and the brevibacterium halotolerans GDSD21 has extensive application prospects in soil improvement, increasing of the soil fertility, ecological restoration of a virgin land, and production of industrialized seedling-rearing inoculation agents, nitrogen-fixing microbial agents and bio-organic fertilizers for vegetables.
Owner:北京复天科技有限公司 +1

Azotobacter-containing microbial fertilizer

The invention discloses an azotobacter-containing microbial fertilizer. The azotobacter-containing microbial fertilizer consists of microbial fermentation broths in percentage by volume; microbes are photosynthetic bacteria, azotobacter chroococcum, phosphate-solubilizing bacillus megaterium, jelly-like bacilli, actinomycetes and lactic acid bacteria; and the volume ratio of the microbial fermentation broths is 1 to 15:2 to 10:5 to 9:1 to 8:2 to 10:3 to 8. The microbial fertilizer can fix nitrogen, solubilize phosphate, increase the survival rate of seedlings and the germination rate of seeds, effectively prevent diseases and pests and enhance the disease resistance of plants.
Owner:THE IT ELECTRONICS ELEVENTH DESIGN & RES INST SCI & TECHNOLOGICAL ENG

Selenium-rich combination azotobacter and preparation method for freezing dry powder, liquid fertilizer and solid fertilizer of the same

The present invention provides a method for producing selenium-enriched combined nitrogen-fixing bacteria agent and its freeze-dry powder, liquid fertilizer and solid fertilizer. The culture medium of selenium-enriched combined nitrogen-fixing bacteria is composed of Na2SeO3, peptone, yeast, rare earth aqueous solution, NaCl, water etc. Three strains are respectively inoculated in flasks. Alcaligenes faecalis is represented by A15, nitrogen-fixing giant bacillus is represented by N13, Enterobacter Cloacae is represented by E26. Liquid bacterial fertilizer is obtained via rocking bottle enlarged cultivation, seed tank culture, liquid ferment in ferment tank, preservation and adjusting pH. If it is processed with vacuum freeze-dry, the freeze-dry powder is obtained. The solid fertilizer culture medium comprises grass charcoal, chicken dung, gypsum, rare earth, KH2PO4,Na2SeO3 5H2O. Fermented bacteria strain in ferment tank is inoculated at solid fertilizer culture medium and the final product is obtained after compost. The present inventive process is practical and suitable for industrial production.
Owner:崔澎

Bacterial manure microcapsule formulation special for mangrove forest, preparation method thereof and special equipment

The invention relates to a special bacterial manure microcapsule preparation for mangrove. The preparation is characterized in that: s: 1) the preparation consists of a degradable micro-granular capsule with azotobacter and phosphate-dissolving bacteria for promoting growth of mangrove; 2) bacterial seeds of the azotobacter for promoting growth of mangrove is cultivated by an OAB culture medium; bacterial seeds of the phosphate-dissolving bacteria for promoting growth of mangrove is cultivated by a phosphate-dissolving bacteria selective culture medium SRSM; and 3) the degradable micro-granular capsule is prepared from stock solution of a wall material, stock solution of a core material, a hardener and a solidifying liquid. The preparation method is characterized by comprising the following steps: 1) the zotobacter and the phosphate-dissolving bacteria for promoting growth of mangrove are collected, sifted and cultured; 2) the microcapsule preparation is prepared; and 3) the bacterial manure microcapsule preparation is stored. The structure of special equipment has the characteristics that the special equipment comprises an air compressor (1), an air filter (2), a bacteria filter (3), a granule spraygun (4), a glue solution bottle (5), a vibrator (7) and a filter (8). The bacterial manure microcapsule preparation has the advantages of simple process, convenient operation, stable product quality, long shelf life and convenient storage and transportation.
Owner:RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY

Soil improvement type compound biologic organic liquid fertilizer

This invention relates to a composite bioorganic liquid fertilizer for soil improvement. The fertilizer is composed of Azotobacteria, Nitrobacteria, phosphorus and potassium solubilizing bacteria, Lactobacilli, photosynthetic bacteria, yeasts, Actinomycetes and growth factor bacteria. The fertilizer is manufactured by selecting the bacteria from soil, culturing by biotechnology, adding organic substances and nutrients, and chelating. The fertilizer can restore soil ecosystem, and improve soil physical and biological properties. The fertilizer can be used on a variety of crops.
Owner:杨雪枣

Biological preparation for treating sodium glutamate fermentation sewage

The invention relates to a biological preparation for treating sodium glutamate fermentation sewage. The biological preparation comprises a physical preparation and a complex microbial agent, wherein the complex microbial agent comprises the following components in parts by weight: 4-5 parts of Azotobacter chroococcum, 4-5 parts of phanerochaete chrysosporium, 5-6 parts of Bacillus amyloliquefaciens, 4-6 parts of Clostridium papyrosolvens, 3-4 parts of penicillium ochrochlorron, 3-4 parts of Pseudomonas aeruginosa and 2-3 parts of bacillus cereus. The biological preparation contains multiple microorganisms having excellent degradation capacity to non-biodegradable pollutants, and by virtue of reasonable compatibility of the strains, the good degradation effect is achieved, and the application prospect of the biological preparation is wide.
Owner:内蒙古阜丰生物科技有限公司

A rice endophytic nitrogen-fixing bacterium that produces acc deaminase and antagonizes scab and its use

InactiveCN102286401AIncrease nitrogenase activityStrong adaptability to competitionPlant growth regulatorsBiocideBurkholderia glatheiMicrobial agent
The invention discloses a rice endotrophic azotobacter for producing activated calcium carbonate (ACC) and realizing the antagonistic effect on fusarium graminearum and a purpose thereof. The rice endotrophic azotobacter is Burkholderia sp. GDSD125 and has the preservation number being CGMCC No.5038 in General Microbiology Center of China Microbial Strain Preservation Management Committee. The Burkholderia sp. GDSD125 CGMCC No.5038 provided by the invention has high nitrogen fixation activity and ACC ammonialyase activity, can realize the effective antagonistic effect on gibberella zeae and has wide application prospects in nitrogen fixation microbial agent and microbial organic fertilizer production.
Owner:INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI

Biochemical preparation for treating glutamic acid fermentation sewage

The invention relates to a biochemical preparation for treating glutamic acid fermentation sewage. The biochemical preparation contains a physical adsorbent and a composite microbial preparation, wherein the composite microbial preparation is prepared from the following raw materials in parts by weight: 6-8 parts of azotobacter chroococcum, 4-6 parts of Rhodococcus sp., 4-6 parts of bacillus amyloliquefaciens, 3-5 parts of clostridium papyrosolvens, 3-5 parts of penicillium ochrochlorron, 3-4 parts of Pseudomonas stutzeri and 2-3 parts of bacillus cereus. The biochemical preparation contains a variety of microbes which have excellent degradation capability to refractory contaminants, and all strains are reasonably combined, so that the biochemical preparation has a good degradation effect and has a broad application prospect.
Owner:内蒙古阜丰生物科技有限公司

Treatment process of monosodium glutamate fermentation wastewater

The invention relates to a treatment process of monosodium glutamate fermentation wastewater. The wastewater is subjected to physical adsorption and composite bacteria treatment, wherein composite bacteria comprises the following components in parts by weight: 7 parts of azotobacter chroococcum, 5 parts of rhodococcus, 5 parts of bacillus amyloliquefaciens, 4 parts of clostridium papyrosolvens, 4 parts of penicillium ochrochloron, 3 parts of pseudomonas aeruginosa and 2 parts of bacillus cereus. As the composite bacteria contain various microorganisms that have an excellent degradation capability for refractory pollutants, and respective bacteria are combined reasonably, a good degradation effect is realized, and the treatment process of the monosodium glutamate fermentation wastewater has a broad application prospect.
Owner:内蒙古阜丰生物科技有限公司

Solid microbial inoculum for remediating Cd in farmland soil

A solid microbial inoculum for remediating Cd in farmland soil, comprising: curtobacterium luteum, cupriavidus necator, sphingomonas parapaucimobilis, cellulosimicrobium cellulans, azotobacter sp., saccharomyces sp., streptomyces sp. and pseudomonas sp. Acetic acid solubility of Cd, Cu and Zn in soil that is remediated by the solid microbial inoculum disclosed by the invention can be decreased significantly.
Owner:SHANGHAI JIAO TONG UNIV

Gene capable of improving the nitrogen fixing ability of combined azotobacter and uses thereof

InactiveCN1500801AImprove carbon metabolismIncrease nitrogen fixation levelsBacteriaSugar derivativesBiotechnologySequence analysis
The present invention relates to one kind of gene capable of raising the nitrogen-fixing level of combined nitrogen-fixing bacteria and its encoding sequence, the sequence encoded polypeptide, and the application of the polypeptide in raising the nitrogen-fixing level of combined nitrogen-fixing bacteria. The present invention clones DNA segment containing dct transporting system and performs the sequence analysis; identifies plant factor inducing promote; and constitutes recombinant combined nitrogen-fixing engineering bacteria. The DNA segment encoded polypeptide the present invention provides may be used in preparing biological nitrogen-fixing fertilizer.
Owner:INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI

Microbial agent capable of preventing and controlling apple tree root rot disease as well as preparation method thereof

The invention discloses a microbial agent capable of preventing and controlling an apple tree root rot disease as well as a preparation method thereof. The microbial agent capable of preventing and controlling the apple tree root rot disease is prepared from the following raw materials in parts by weight: 20 to 30 parts of trichoderma harzianum, 10 to 20 parts of bacillus natto, 15 to 25 parts ofbacillus subtilis, 20 to 30 parts of hansenula anomala, 5 to 15 parts of azotobacter chroococcum and 5 to 10 parts of porous magnetic chitosan microsphere. The microbial agent capable of preventing and controlling apple tree root rot disease completely avoids the problem of chemical medicine resistance caused by use of chemical agents, so that the microbial agent is favorable for pollution-free production of apples. A biological flora, which is complex, stable in structure and wide in function and has various bacterial microflora, is formed by depending on the mutual synergic effect and proliferation relationship and has a good prevention and control effect on the apple tree root rot disease.
Owner:湖北茂盛生物有限公司

Heavy metal contaminated soil remediation agent and soil remediation method

The invention discloses a heavy metal contaminated soil remediation agent and a soil remediation method. The remediation agent comprises the following components in parts by weight: 50-80 parts of treated activated carbon, 3-10 parts of zeolite, 10-20 parts of marl, 20-30 parts of grape wine waste liquid and 0.05-1 part of azotobacter chroococcum. The remediation agent disclosed by the invention utilizes the grape wine waste liquid, is environment-friendly and economic, can remarkably remediate heavy metal pollution in soil, does not generate secondary pollution, and is simple in operation process, short in period and quick in effect.
Owner:CHENGDU TECHCAL UNIV

Mangrove rhizosphere growth promoting azotobacter (DZY-HS14) and use thereof

The invention provides a mangrove plant rhizosphere growth-promoting nitrogen fixing bacterium (DZY-HS14) and an application thereof. The bacterium is the bacillus sp. DZY-HS14 screened from the rhizosphere settlings of the tropical mangrove plants and contains 16SrDNA serial and nifH and is stored in the CCTCC; the collection number is CCTCCNO: M207043. The strain has high biological nitrogen fixing activity; the microbial inoculum with the bacterium is used to breed mangrove plants such as rhizophora stylosa and bruguiera, which can evidently promote the growth of plants, thereby being capable of preparing the biological nitrogen fixing bacterium fertilizer promoting the growth of mangrove plants.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI

Anti-inverse growth substance of plant and its prepn. method

A stress-resistance growth promoter for plant is prepared from natural minerals, azotobacteria, photosynthesizing bacteria, and chitosan through proportionally mixing. Its advantage is high effect to promote the growth and development of plant, and improve the resistance to stress and diseases.
Owner:HEYING BIOENG GUANGDONG +2

Mangrove plant growth promoting azotobacter (DZY-X1) and use thereof

The invention provides a mangrove plant rhizosphere growth-promoting nitrogen fixing bacterium (DZY-X1) and an application thereof. The bacterium is the vibrio sp. 2006-DZY-X1 screened from the rhizosphere settlings of the tropical mangrove plants and contains 16SrDNA serial and nifH and is stored in the CCTCC; the collection number is CCTCCNO: M207118. The strain has high biological nitrogen fixing activity; the microbial inoculum with the bacterium is used to breed mangrove plants such as rhizophora stylosa and bruguiera, which can evidently promote the growth of plants, thereby being capable of preparing the biological nitrogen fixing bacterium fertilizer promoting the growth of mangrove plants.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI

Microbial and enzyme powder composite biological preparation for promoting crop yield increasing

The invention relates to the technical field of crops, in particular to a microbial and enzyme powder composite biological preparation for promoting crop yield increasing. Raw materials of the preparation include, by weight, 3-6 parts of azotobacter, 1-2 parts of cellulase, 3-6 parts of Paecilomyces lilacinus, 7-10 parts of photosynthetic bacterial floras, 1-5 parts of Bacillus licheniformis, 2-4parts of Aspergillus oryzae, 5-8 parts of Bacillus laterosporus, 12-15 parts of mycorrhizal fungi, 1-3 parts of invertase, 0.5-2 parts of hydrolase and 1-4 parts of phosphatase. The preparation has characteristics of increasing crop yields, improving soil organic matters, improving soil structures and promoting animal and crop growth.
Owner:FUYANG NORMAL UNIVERSITY

Composite agricultural microorganism bacteria and method for applying composite bacteria to lotus root planting

The invention provides composite agricultural microorganism bacteria and a method for applying composite bacteria to lotus root planting. All strains of compound bacteria comprise, by weight, 20% of rhodospirillum, 10% of nitrospira, 10% of azotobacter, 10% of bacilli, 10% of thermoactinomyces, 10% of xanthomonas, 10% of lysobacter, 10% of acidophilic bacteria and 10% of novosphingobium. The composite bacteria are used for lotus root planting, the yield of lotus roots is increased, and the quality of the lotus roots is improved.
Owner:NANPING CP OREEZYME BIOTECH DEV CO LTD

Compounded amino-acid microbial bacterium agent and preparation method therefor

ActiveCN105198567AHigh content of effective live bacteriaLow bacteria rateFertilizer mixturesBiotechnologyAzotobacter chroococcum
The invention provides a compounded amino-acid microbial bacterium agent and a preparation method therefor. The microbial bacterium agent is prepared from the raw material ingredients in parts by weight: 38 parts of Sphingomonas yanoikuyae powder, 12 parts of Lactobacillus bulgaricus powder, 25 parts of Azotobacter chroococcum powder, 20 parts of Bacillus laterosporus powder, 9 parts of compounded amino acid salt, 20 parts of zymosan and 26 parts of choline chloride. The compounded amino acid salt contains L-histidine salt, L-cysteine salt, L-ornithine salt and L-lysine-L-aspartate, wherein the weight content ratio of the L-histidine salt to the L-cysteine salt to the L-ornithine salt to the L-lysine-L-aspartate is 5: 3: 2: 2. The compounded amino-acid microbial bacterium agent provided by the invention has the pH value of 6.8 and the moisture content of 18-22%; the content of effective living bacteria is 6.6-7.9*10<9> / g, and the ratio of miscellaneous bacteria is 3.2-4.7%.
Owner:寿光市浩鑫环境治理有限公司

Eco-fertilizer produced by kitchen waste and eco-fertilizer thereof and production method thereof

The invention discloses an eco-fertilizer produced by kitchen waste. The eco-fertilizer comprises the following raw materials in parts by weight: 90-95 parts of a matrix material, 5-10 parts of combined azotobacteria, and 5-10 parts of clay. The matrix material is at least one of kitchen waste, cow dung and pig dung. The eco-fertilizer takes urban and rural residents kitchen waste, livestock manures such as cow dung and pig dung, and combined azotobacteria as main materials, the clay is taken as a binding material, a spherical fertilizer ball with upper and lower through holes as well as the square through holes is produced, the fertility release speed of the fertilizer is alleviated, and fertilizer utilization efficiency is effectively increased.
Owner:张政权
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