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Method for improving liposome stability

A liposome and stability technology, applied in the field of improving liposome stability, to achieve high stability, reduce leakage, and improve release properties

Inactive Publication Date: 2013-06-26
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The liposome prepared by the present invention overcomes defects such as fusion, rupture and precipitation which are easy to occur in the current liposome in the process of preparation, storage and use, improves the stability of the liposome, and reduces the concentration of the drug in the liposome. Leakage, which helps improve drug release properties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Silica nanoparticles, water, cetyltrimethylammonium bromide, egg yolk lecithin and chloroform are measured in a mass ratio of 1:125:15:1:100. Measure silica nanoparticles, cetyltrimethylammonium bromide and water according to the above ratio, mix them evenly, and disperse them by ultrasonic for 5 minutes. Measure phospholipids and chloroform according to the above ratio, and mix well. Under the condition of stirring, the mixed solution of silicon dioxide nanoparticles, cetyltrimethylammonium bromide and water is added dropwise to the mixed solution of phospholipid and chloroform to obtain the water-in-oil emulsion. Then, water is added to the water-in-oil emulsion to obtain a water-in-oil-in-water emulsion. The quality of the water added therein is 10 times of that of the water-in-oil emulsion. The water-in-oil-in-water emulsion is evaporated under reduced pressure to remove the chloroform, and liposomes encapsulating silicon dioxide nanoparticles are obtained. Test ...

Embodiment 2

[0017] Silica nanoparticles, water, cetyltrimethylammonium bromide, soybean lecithin and chloroform are measured in a mass ratio of 1:150:20:3:120. Measure silica nanoparticles, cetyltrimethylammonium bromide and water according to the above ratio, mix them evenly, and disperse them by ultrasonic for 10 minutes. Measure phospholipids and chloroform according to the above ratio, and mix well. Under the condition of stirring, the mixed solution of silicon dioxide nanoparticles, cetyltrimethylammonium bromide and water is added dropwise to the mixed solution of phospholipid and chloroform to obtain the water-in-oil emulsion. Then, water is added to the water-in-oil emulsion to obtain a water-in-oil-in-water emulsion. The quality of the water added therein is 15 times of that of the water-in-oil emulsion. The water-in-oil-in-water emulsion is evaporated under reduced pressure to remove the chloroform, and liposomes encapsulating silicon dioxide nanoparticles are obtained. Test ...

Embodiment 3

[0019] Silica nanoparticles, water, cetyltrimethylammonium bromide, dipalmitoylphosphatidylcholine and chloroform are measured in a mass ratio of 1:200:30:5:150. Measure silica nanoparticles, cetyltrimethylammonium bromide and water according to the above proportions, mix them evenly, and disperse them by ultrasonic for 15 minutes. Measure phospholipids and chloroform according to the above ratio, and mix well. Under the condition of stirring, the mixed solution of silicon dioxide nanoparticles, cetyltrimethylammonium bromide and water is added dropwise to the mixed solution of phospholipid and chloroform to obtain the water-in-oil emulsion. Then, water is added to the water-in-oil emulsion to obtain a water-in-oil-in-water emulsion. The quality of the water added therein is 15 times of that of the water-in-oil emulsion. The water-in-oil-in-water emulsion is evaporated under reduced pressure to remove the chloroform, and liposomes encapsulating silicon dioxide nanoparticles ...

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PUM

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Abstract

The invention relates to a method for improving liposome stability, which comprises the following steps of: firstly, measuring the following components by mass ratio: 1 part of silicon dioxide nanoparticle, 125-250 parts of water, 15-50 parts of hexadecyl trimethyl ammonium bromide, 1-10 parts of phosphatide and 100-200 parts of trichloromethane; secondly, uniformly mixing the silicon dioxide nanoparticle and the hexadecyl trimethyl ammonium bromide with the water, and performing ultrasonic dispersion for 5-20 minutes to obtain a mixed solution A; uniformly mixing the phosphatide with the trichloromethane to obtain a mixed solution B; thirdly, dropwise adding the mixed solution A to the mixed solution B to obtain a W / O emulsion; then, adding water to the W / O emulsion to obtain a W / O / W emulsion; and finally, performing vacuum evaporation to remove the trichloromethane to obtain liposome which encapsulates the silicon dioxide nanoparticle. According to liposome, the defects of easy fusion, fracture, precipitation and the like existing in conventional liposome are overcome, the liposome stability is improved, and drug release property improving and drug bioavailability increasing arefacilitated.

Description

technical field [0001] The invention relates to a method for improving liposome stability. It belongs to the field of medical technology. Background technique [0002] Liposomes are spherical, closed vesicles formed of phospholipid molecules. Phospholipid molecules contain two hydrophobic fatty acid chains and a hydrophilic head group. When dispersed in water, phospholipid molecules form a bilayer structure. Because the basic structure of biological plasma membrane is also phospholipid bilayer, liposome has a structure similar to that of biological cells, so it has good biocompatibility. After the liposome enters the human body, it will activate the immune mechanism of the human body and be swallowed by the reticuloendothelial system, thereby enriching targetedly in tissues such as liver, spleen, lung and bone marrow. The liposome preparation process is simple, the biocompatibility is good, and there is no immune reaction. In addition, liposomes can encapsulate both fat-s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A61K47/04
Inventor 刘明贤甘礼华陈柳华
Owner TONGJI UNIV