High-flux cell culture apparatus and manufacturing method thereof

Abstract

The invention discloses a high-flux cell culture apparatus and a manufacturing method thereof. The high-flux cell culture apparatus comprises a cell culture room which is formed by a plurality of independent cell culture layers which are isolated from one another by clapboards; each cell culture layer is internally provided with a culture medium feed and discharge hole and a sealing plug; a ventilation port provided with a filter is inserted into the rear of the sealing plug; the culture medium feed and discharge hole is connected with a feeding device by a pipeline; the feeding device is respectively connected with a culture solution storage tank and a cell collection bottle by the pipeline; and a feed power device is a single-tube or multi-tube peristaltic pump which is used for conveniently adjusting the flow velocity and the flow rate. The high-flux cell culture apparatus can be used for confirming the cell culture dose according to the need and can conveniently switch different culture mediums, thus meeting the demands of different cell culture stages and conveniently adjusting the cell culture dose; and the high-flux cell culture apparatus can be used for research and development as well as actual production, and has wide application scope.

Description

technical field The invention relates to a cell culture vessel and a preparation method thereof, in particular to a high-throughput cell culture vessel and a preparation method thereof. Background technique In the past ten years, due to the rapid development of the biological product industry, the traditional method of obtaining biological products from animal tissues through biochemical technology has been far from meeting the needs of the market. Animal cells are cultured in vitro to express specific proteins, Monoclonal antibodies, interferons and viral vaccine preparations have become the most common technologies at present. In addition, in recent years, with the development of stem cell technology, the application of human cell culture has become more and more extensive. Among them, tissue and organ reconstruction and cell therapy have become the most promising methods for the treatment of human diseases. Cell therapy refers to a treatment method in which human autolog...

AI Technical Summary

Problems solved by technology

But it also has its disadvantages: high labor intensity, large floor space, small surface area for cell growth per unit volume, low cell growth density, and difficult control of differences between bottles, etc., so it is difficult to industrialize or scale production

However, this system fails to solve the problems of non-destructive recovery of cells after culture and simultaneous culture of multiple strains of cells in a reactor

In addition, the system cannot simultaneously culture multiple batches or strains of cells in the same cell culture device;

Conventional cell culture dishes, plates, and bottles are all open, and contamination is prone to occur during liquid addition, inoculation, and cell harvesting operations

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