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Cellulase and application thereof

A technology of cellulase and amino acid, applied in the direction of applications, enzymes, enzymes, etc., can solve problems such as poor heat resistance

Inactive Publication Date: 2013-08-21
东莞泛亚太生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even though cellulase has been widely used in industry for a long time, many industrial cellulase are screened from mesophilic bacteria such as Trichoderma reesei, so they have poor heat resistance

Method used

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  • Cellulase and application thereof
  • Cellulase and application thereof
  • Cellulase and application thereof

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Embodiment Construction

[0019] Embodiments of the features and advantages of the present invention will be described in detail herein. It should be understood that the present invention will have various changes under different implementations, but the changes do not depart from the protection scope of the present invention, and the description and drawings therein are to illustrate the present invention in essence, rather than limit the invention.

[0020] In order to increase the industrial application value of heat-resistant cellulase, the present invention screens out the Cel12A gene from Thermotoga maritima, one of the high-temperature-resistant strains, and the cellulase protein expressed by it is a glucan Carbohydrase (β-1, 4-endoglucanase), analyze the three-dimensional structure of the cellulase protein, then analyze the active region of this structure in detail, and mutate the key amino acids into other amino acids, thus The properties of the protein are modified to further improve the act...

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Abstract

The invention relates to cellulase. The cellulase comprises a mutant amino acid sequence of which the tyrosine at the 61th site of Cel12A zymoprotein is substituted by another amino acid, wherein the amino acid for substitution is selected from phenylalanine, alanine or glycine. The cellulase can be used for food industry, feed industry, textile industry, papermaking industry or biomass energy industry.

Description

technical field [0001] The invention relates to a cellulase, in particular to a heat-resistant cellulase, and also relates to the application of the cellulase. Background technique [0002] Cellulose is the main component of plant cell walls and the main source of biomass energy on the earth. Therefore, many enzyme proteins that can effectively decompose cellulose are widely used in different industries. [0003] Cellulose is a long-chain polysaccharide formed by linking glucose molecules through β-1,4-glycosidic bonds (β-1,4-glycosidic bond) as a unit. These polysaccharides are organized together into compact crystalline cellulose , and thus resist the decomposition of the outside world. However, many herbivores and microorganisms in the biological world need to decompose the polysaccharide cellulose in the plant cell wall into glucose monosaccharides that can be absorbed by the body as a source of energy for survival. For example, there are many different commensal micro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12N15/56C12N15/63
Inventor 郭瑞庭郑雅珊吴姿慧黄建文赖惠琳林正言
Owner 东莞泛亚太生物科技有限公司