Sperm quality evaluation method based on flow cytometry

Abstract

The invention discloses a sperm quality evaluation method based on flow cytometry, which comprises the following steps of: firstly, commonly dyeing a semipermeable membrane nucleic acid fluorescent dye, a non-semipermeable membrane fluorescent nucleotide dye and a sample to be detected; and then, incubating a fluorescent marker monoclonal antibody and the dyed sample to be detected and carrying out flow cytometric detection to obtain the final detection results,. The fluorescent marker monoclonal antibody can be reacted with a sperm acrosome membrane. The laser excitation wavelength differences between the fluorescent marker monoclonal antibody and the semipermeable membrane fluorescent nucleotide dye and between the fluorescent marker monoclonal antibody and the non-semipermeable membrane fluorescent nucleotide dye are 100-200 nm, and the fluorescent scattering wavelength differences are 75-150 nm. The sperm quality evaluation method has the beneficial effects through one-time detection, a plurality of parameter results can be simultaneously obtained, so that the apoptosis rate, the plasma membrane integrity rate and the survival rate of sperm are obtained, and the detection labor strength is reduced; and the demands on a large batch of sample detection in the clinical can be satisfied.

Description

technical field

[0001] The invention belongs to the technical field of medicine and relates to a sperm quality evaluation method based on flow cytometry. Background technique

[0002] Sperm quality assessment is an important indicator of male fertility. The traditional sperm quality assessment technology is to use a phase contrast microscope and a special blood cell counting panel to observe with the naked eye, mainly to evaluate and analyze the sperm count, sperm motility and sperm morphology. In the past two decades, with the continuous advancement of computer technology, special analysis software combined with high-resolution image acquisition devices has appeared clinically to evaluate and analyze sperm quality. The main evaluation indicators are the same as the traditional sperm quality evaluation methods. Although the computer-aided sperm quality assessment method (CASA) reduces the work intensity of visual assessment, especially the assessment of sperm motility is m...