Method for preparing copper polysaccharide through radial flow coupling ultra-filtering separation

Abstract

The invention discloses a method for preparing copper polysaccharide through radial flow coupling ultra-filtering separation. The method comprises the following steps of: utilizing radial flow chromatography to remove protein, thereby purifying coarse copper polysaccharide; utilizing ultra-filtration to separate the purified copper polysaccharide into three components, namely, below 10KD, from 10KD to 100KD, and above 100KD; further removing the protein through ultra-filtration; taking the extracting solution of the copper polysaccharide obtained through separation and purification as liquid material for tobacco and adding onto tobacco shred; and generating a specific chemical component when the tobacco is burnt and smoked, thereby obviously increasing or improving the quality of the tobacco. Compared with the operation of adding the coarse extracting solution to the tobacco shred, the method has the advantages that a certain excitant while smoking is reduced and the taste and the smoking feel are more obviously improved. The method provided by the invention is low in production cost, is pollution-free, is high in separating and purifying speed, is excellent in effect and is suitable for industrial production. To some extent, the defect of dry smoke of Chinese tobacco product is improved according to the method and the method has practical production significance and value.

Description

1. Technical field [0001] The invention relates to a method for preparing copper algae polysaccharide through radial flow coupled ultrafiltration separation and its application. 2. Background technology [0002] Copper algae, commonly known as "Lilac House" (Nanji Island, China), belongs to the genus Sargassum. Copper algae is a warm-temperate seaweed unique to the western North Pacific Ocean. It is distributed discontinuously along the coast of my country. It is an important raw material for extracting alginate. It is widely used in medicine, food, feed and organic fertilizer because it is rich in beneficial biogenic elements. Studies have shown that copper algae contain alginic acid, brown algae starch, mannitol and various amino acids, polysaccharides, proteins, vitamins, etc. Copper algae has the effects of eliminating phlegm, softening hard masses, clearing away heat and diuresis. In recent years, domestic and foreign experts' research on copper algae mainly focuses o...

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Problems solved by technology

[0003] The research on the separation and purification of polysaccharides has made great progress in the research of polysaccharides, a high molecular polymer, in the field of life sciences. The traditional separation and purification of polysaccharides uses axial flow chromatography. The disadvantage of this technology is that the separation and purification steps are complicated and require The time is longer; while the purification of polysaccharides by radial flow has a large processing capacity and short processing time, but the purification effect is low, such as the general removal rate of proteins is about 80%; using ultrafiltration technology to purify polysaccharides, different ultrafiltration membranes The purification efficiencies are different, but the removal rate of protein with higher purification efficiency is only about 80%, which cannot meet the requirements of tobacco additives

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