Polygalacturonase PCIPG21 from phytophora capsic, and coding gene and application thereof

A technology of encoding and amino acids, which is applied in the fields of application, genetic engineering, and plant genetic improvement, and can solve problems such as agricultural production losses
CN102649953AInactive Publication Date: 2012-08-29SHANDONG AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANDONG AGRICULTURAL UNIVERSITY
Publication Date
2012-08-29
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses polygalacturonase PCIPG21 from phytophora capsic, and a coding gene and application thereof. The polygalacturonase PCIPG21 is the following protein shown in a), b) or c): a) the protein comprises the amino acid sequence shown as the 20th to the 390th site of the sequence 2 in the sequence table; b) the protein comprises the amino acid sequence shown as the sequence 2 in the sequence table; and c) the protein is derived from a) and formed by substituting and / or deleting and / or adding one or more amino acid residues in the amino sequence shown as the sequence 2 in the sequence table or the 20th to the 390th site of the sequence 2 in the sequence table, and has the polygalacturonase activity. The polygalacturonase PCIPG21 has high enzyme activity, wherein the specificactivity of the polygalacturonase is 63 + / - 0.2 U / mg protein; and the specific activity of pectinase is 43 + / - 0.2 U / mg.
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Description

technical field

[0001] The invention relates to a polygalacturonase, its encoding gene and application, in particular to the polygalacturonase from Phytophthora capsici and its encoding gene and application. Background technique

[0002] During the interaction between plant pathogenic oomycetes and hosts, they secrete a variety of important cell wall degrading enzymes or pathogenic enzymes, among which polygalacturonase (PG: EC 3.2.1.15) is a kind of enzyme secreted by various plant pathogenic oomycetes. Important pathogenic enzymes, this type of enzyme destroys the host defense system by degrading the pectin and mesogelatin of the host plant cell wall, thereby enhancing the affinity of the pathogen to the host, so this type of enzyme is an important pathogenic factor. Studies have shown that polygalacturonase is a cell wall-binding protease that can catalyze the cleavage of α-(1,4)-polygalacturonic acid in pectin molecules, degrade pectin in the cell wall, and lead to softe...

Claims

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