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Remediation method of soil polycyclic aromatic hydrocarbon by combining surfactant producing bacteria and mycorrhiza

A technology of surfactants and polycyclic aromatic hydrocarbons, which is applied in the fields of pollutant treatment and environmental restoration, and can solve problems such as difficult large-scale application, easy degradation, and high cost of biosurfactants

Inactive Publication Date: 2012-09-05
JIANGSU UNIV
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Problems solved by technology

[0005] However, due to the disadvantages of high cost and easy degradation, biosurfactants are difficult to be applied on a large scale in the actual restoration of soil organic matter.

Method used

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  • Remediation method of soil polycyclic aromatic hydrocarbon by combining surfactant producing bacteria and mycorrhiza
  • Remediation method of soil polycyclic aromatic hydrocarbon by combining surfactant producing bacteria and mycorrhiza
  • Remediation method of soil polycyclic aromatic hydrocarbon by combining surfactant producing bacteria and mycorrhiza

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Embodiment Construction

[0026] The Bacillus subtilis B. subtilis BS1 of the present invention was purchased from Zhenjiang Weisai Biotechnology Development Co., Ltd.

[0027] The mycorrhizal fungus Glomus etunicatum was purchased from Glomeromycota In vitro Collection (GINCO), Belgium.

[0028] PAHs can be divided into many types according to the number of benzene rings. Below with the degradation of phenanthrene as specific example, its process is as follows figure 1 Shown:

[0029]

[0030] Phenanthrene molecular structure

[0031] Step 1, Bacillus subtilis B. subtilis BS1 amplification culture and bacterial agent preparation

[0032] Bacillus subtilis B. subtilis Yeast extract peptone liquid medium for BS1 (tryptone 10 g / L, yeast extract 5 g / L, sodium chloride 10 g / L), at 30 o C, cultured at a rotational speed of 180 rpm. When the bacterial cells grow to the late logarithmic growth phase, the bacterial cells are collected to prepare the seed solution. The seed solution is expanded an...

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Abstract

The invention discloses a remediation method of soil polycyclic aromatic hydrocarbon by combining surfactant producing bacteria and mycorrhiza, belonging to the field of environmental modification and pollutant disposal. Based on the actual bottleneck of current phyto-remediation or mycorrhiza remediation, by combining the high-production strain B.subtilis BS1 of the biosurfactant surfactin with AMF, the functions and characteristics of the two are sufficiently realized, wherein the B.subtilis BS1 is used as the donor of the biosurfactant and can enhance the migration of the organic pollutant toward the rhizosphere; and AMF enhances the affinity and absorption effect of the plant against the organic pollutant, improves the tolerance of the plant against the toxicity stress of the organic pollutant, and enhances the metabolic activity of the rhizospheric microorganism. According to the invention, a novel efficient integrated remediation technology is created, the remediation efficiency of PAHs in soil is improved, the remediation time is shortened, and the technology has great practical application values.

Description

technical field [0001] The invention relates to a technology for repairing polycyclic aromatic hydrocarbons in soil by combining surfactant-producing bacteria and mycorrhizae, and belongs to the fields of environmental restoration and pollutant treatment. Background technique [0002] As coal and petroleum are widely used in industrial production, transportation and daily life, polycyclic aromatic hydrocarbons (PAHs) have become organic pollutants of common concern to all countries in the world. The Chinese government has listed 7 PAHs in the "China Environmental Priority Pollutants Blacklist". Due to its special chemical structure, PAHs pollutants are easy to accumulate in organisms and have high biological toxicity. However, such pollutants have strong adhesion in the soil and are difficult to remove and degrade. Therefore, the accumulation of PAHs in the soil has become an increasingly serious threat to agricultural production, food safety, and human health. [0003] Th...

Claims

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Application Information

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IPC IPC(8): B09C1/10
Inventor 肖翔司春灿林英杜道林曹丹鸣王明娜
Owner JIANGSU UNIV
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