Oyster yellow wine
A technology for oysters and rice wine is applied in the field of health-care functional oyster rice wine, which can solve the problems of incomplete addition of nutrients, and achieve the effects of moderate alcohol content, simple process, and improved nutrition and health-care functions.
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Embodiment 1
[0031] Fresh oysters are shelled and washed, black edges and viscera are removed, weigh about 300g, add a little water, mash with a masher, and then homogenize with a high-shear homogenizer to collect the homogenate. Add water 5 times the weight of oysters to prepare medium and sterilize at 100°C.
[0032]Inoculate the purified Bacillus cereus strains preserved in cold storage or vacuum freeze-drying into the seed medium (peptone 1%, yeast extract 0.5%, sodium chloride 1%, natural pH), and place in a 37°C incubator Cultivate for 24 hours, then inoculate it into the seed medium with 2% inoculum amount and incubate at 37°C for 18 hours, centrifuge to discard the supernatant, add sterilized physiological saline equal to the volume of the culture medium, and centrifuge again to wash the bacterial cells ; Then, add 3-5mL sterilized physiological saline to the thalli that discarded the supernatant, oscillate and mix well, and make an activated seed solution. The activated seed solu...
Embodiment 2
[0035] Fresh oysters are shelled and washed, black edges and viscera are removed, weigh about 300g, add a little water, mash with a masher, and then homogenize with a high-shear homogenizer to collect the homogenate. Add water 5 times the weight of oysters to prepare medium and sterilize at 100°C.
[0036] Inoculate brewer’s yeast strains that have been purified and stored in refrigeration or vacuum freeze-drying into seed medium (0.5% peptone, 1.5% yeast extract, 0.4% sodium chloride, 1% glucose, pH 7.0), and place in a 30°C incubator Cultivate in medium for 24 hours, then inoculate it into the seed medium with 2% inoculum amount and incubate at 30°C for 24 hours, centrifuge and discard the supernatant, add sterilized physiological saline equal to the volume of the culture solution, and centrifuge again to wash the bacterial cells; Then, add 3 to 5 mL of sterilized physiological saline to the bacterial cells that discarded the supernatant, shake and mix well to make an activa...
Embodiment 3
[0039] Fresh oysters are shelled and washed, black edges and viscera are removed, weigh about 300g, add a little water, mash with a masher, and then homogenize with a high-shear homogenizer to collect the homogenate. Add water 5 times the weight of oysters to prepare medium and sterilize at 100°C.
[0040] The aspergillus oryzae strains that had been purified, refrigerated or vacuum freeze-dried were transferred to the test tube bean juice slant medium, and cultured in a biochemical incubator at 30°C for 96 h; the activated strains were transferred to On the eggplant bottle bean juice slant medium, expand the culture in a biochemical incubator at 30 °C for 96 h; use a 100 mL triangular flask for culture (soybean meal: drum skin = 1:3, moisture content 52%, sterilize at 121 °C for 30 min), and expand the culture Aspergillus oryzae made spore suspension, set aside. Inoculate the activated seed liquid into the prepared oyster culture medium according to the inoculation amount of...
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