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Method for synthesizing 3-hydroxybutyrate and 3-hydroxyvalerate copolymer

A technology of hydroxyvaleric acid copolymer and hydroxybutyric acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low product yield

Active Publication Date: 2014-03-26
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The object of the present invention is to provide a kind of 3-hydroxybutyric acid and 3-hydroxybutyric acid for the problem that the product yield that exists in precursor is not high with levulinic acid in the synthetic process of current 3-hydroxybutyric acid and 3-hydroxyvaleric acid copolymer. The synthetic method of hydroxyvaleric acid copolymer

Method used

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  • Method for synthesizing 3-hydroxybutyrate and 3-hydroxyvalerate copolymer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Transfer the bacterial lawn on the fresh slant to 100mL seed medium, culture on a shaking table at 30°C, and rotate at 200r / min. After 20h, transfer it to a 2L fermenter with 1.2L fermentation medium at an inoculum size of 5%. continue to cultivate. The culture temperature is 30°C, the dissolved oxygen is controlled above 30%, and the pH is controlled at 7. Determination of the bacterial dry weight and product concentration of the fermentation broth at different times, the results are as follows: figure 1 shown. From figure 1 It can be seen that at 56 hours of fermentation, the dry weight of the bacteria and the concentration of the product reached the maximum, respectively 15.53g / L and 12.61g / L, and the product content reached 81.2% of the dry weight of the bacteria. Wherein the percentage content of hydroxyvaleric acid in the product is 53.9%.

[0028] Seed medium (g / L): beef extract 10, tryptone 10, yeast powder 2, distilled water to volume, pH 7.0.

[0029] Fer...

Embodiment 2～3

[0032] As in the culture conditions described in Example 1, seeds of different ages were used in the fermentation culture to synthesize PHBV. The difference is that the seed culture time is 16 and 24 hours respectively.

Embodiment 4～5

[0034] As in the culture conditions described in Example 1, different inoculum sizes were used in the fermentation culture to synthesize PHBV. The difference is that the inoculation amount is 10% and 20% respectively.

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Abstract

The invention relates to a method for synthesizing a 3-hydroxybutyrate and 3-hydroxyvalerate copolymer, and relates to a 3-hydroxybutyrate and 3-hydroxyvalerate copolymer. The method includes transferring fresh lawn on an inclined plane onto a seed culture medium to culture, and then transferring the lawn to a fermenting culture medium to ferment so as to obtain fermenting liquid accumulated with the 3-hydroxybutyrate and 3-hydroxyvalerate copolymer; and centrifuging the fermenting liquid and removing liquid supernatant so as to obtain sedimentat, namely thallus of alcaligenes eutrophus; freezing the thallus of the alcaligenes eutrophus, adding acetone in the thallus of the alcaligenes eutrophus, removing liquid supernatant after centrifuging, washing sediment and then mixing the sediment with EDTA (ethylene diamine tetraacetic acid) solution and sodium hypochlorite, centrifuging reacted mixed liquid, removing liquid supernatant, and washing and drying sediment so as to obtain the 3-hydroxybutyrate and 3-hydroxyvalerate copolymer.

Description

technical field [0001] The invention relates to a copolymer of 3-hydroxybutyric acid and 3-hydroxyvaleric acid, in particular to a method for synthesizing the copolymer of 3-hydroxybutyric acid and 3-hydroxyvaleric acid by taking levulinic acid as a precursor. Background technique [0002] 3-Hydroxybutyric acid and 3-hydroxyvaleric acid copolymer (PHBV) is one of the biodegradable plastic polyhydroxyalkanoates (PHAs) produced by microbial fermentation. With the oil energy crisis and people's increasing concern about the environmental pollution caused by chemically synthesized plastics, the research on biodegradable plastics has developed rapidly. become a research hotspot. Polyhydroxyalkanoates are a class of polyesters accumulated by microorganisms under unbalanced growth conditions, among which poly-3-hydroxybutyrate (PHB) was the first to be discovered and studied. PHB is a thermoplastic material with high melting point crystallization similar to polypropylene, but due ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/62C12R1/01
Inventor 何宁陈荣辉李清彪王远鹏彭雅娟黄耀华
Owner XIAMEN UNIV