Method for producing seed by using three-line hybrid rice sterile line with purple leaf marker

Abstract

The invention discloses a method for producing a seed by using a three-line hybrid rice sterile line with a purple leaf marker, comprising the following steps of: (1) arranging the sowing time according to the leaf difference and the time difference of the sterile line and the selected restoring line; (2) raising rice seedlings by using a rice seedling bed; (3) arranging an impurity removal work for a female parent rice field in a rice seedling period, and carrying out water and fertilizer management for a male parent and the female parent, wherein a female parent belongs to the sterile line of a purple leaf, therefore, plants with green leaves in the female parent rice field are needed to be pulled up; (4) enabling the ratio of female parent rows to male parent rows in a seed field to be 2:(12-14) and the number of the male parent rows to be 2 in different sowing times, and transplanting male parent plants into a row shaped like a Chinese character pin; (5) spraying Berelex; (6) carrying out artificial supplementary pollination; (7) isolating to prevent hybrid; (8) strengthening the water and fertilizer management and pest control works for the seed field; and (9) mechanically harvesting the male parent in advance. The method has the advantages of time and labor saving and cost reduction. The sterile line is provided with the purple leaf marker; labor can be saved due to the convenient impurity removal work; and mechanical harvesting is adopted, so that the production cost is reduced.

Description

technical field [0001] The invention belongs to the field of agricultural technology, and relates to cultivating a three-line sterile line of hybrid rice with purple leaf marks, using the sterile line for hybrid rice seed production, and quickly removing impurities and maintaining purity by identifying leaf color characteristics during the seed production process. Improve hybrid purity. Background technique [0002] Due to the obvious heterosis of hybrid rice, its yield, resistance, adaptability, etc. are superior to general conventional rice varieties, so it has been widely popularized. At present, the annual planting area of ​​hybrid rice in my country accounts for about half of the total planting area of ​​rice, while the output accounts for 58% of the total rice output. Despite the rapid development of hybrid rice, the purity of hybrid rice has decreased due to reasons such as self-fertilization and weather effects, resulting in huge losses. Methods for identifying the...

AI Technical Summary

Problems solved by technology

Despite the rapid development of hybrid rice, the purity of hybrid rice has decreased due to self-fertilization, weather effects, etc., causing huge losses

Methods for identifying the purity of hybrid seeds include off-site planting identification, isoenzyme electrophoresis identification, and DNA molecular marker identification, etc. These methods can more accurately identify the purity of hybrids, but the steps are cumbersome, time-consuming and labor-intensive, and the cost is high

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