Application of penicillin to simple test-tube breeding of grapes and novel technology for test-tube breeding of grapes

Abstract

The invention relates to application of penicillin to the simple test-tube breeding of grapes and a novel technology for test-tube breeding of the grapes. The technology comprises the following steps of: removing viruses from grapes which are selected from the field so as to optimize plants, acquiring aseptic seedlings by using a comprehensive nondestructive disinfection technology, performing primary culture on a GS agar culture medium under the illumination condition that the temperature is 25 to 28 DEG C and the illumination is 1,500 to 3,000Lx, cutting into single-bud stem segments and transferring into another GS agar culture medium for subculture when test-tube plantlets grow to a bottle mouth, culturing for 18 to 20 days by using a GN nutrient solution plus vermiculite culture medium added with low-concentration penicillin under the illumination condition that the temperature is 25 to 28 DEG C and the illumination is 1,500 to 3,000Lx two months before transplantation, and transplanting after seedlings are obtained. The technology is simple and convenient, is low in cost, cannot cause the pollution easily, can control sundry fungi, and can also effectively and quickly promote the growth and rooting of the grapes and increase propagation speed.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to the application of penicillin in the simple propagation of grape test tubes and the new technology of grape test tube propagation. Background technique [0002] Test-tube virus-free propagation of grapes is the most effective biotechnology. Test-tube propagation of a virus-free or newly-bred variety and a rare grape variety can quickly provide a large number of neat and consistent virus-free grape seedlings in a short period of time, but test-tube propagation requires certain Advanced equipment and technical means, high cost (the cost of sugar-containing agar medium is 4-8 times of conventional propagation) (see Cao Xiaodong, Cao Ziyi, 1996, cost and benefit analysis of plant test tube propagation. Plant Physiology Communication, 32 (4) , 284-291); On the sugar agar medium, it is easy to breed microorganisms and cause pollution; the test tube seedlings have n...

AI Technical Summary

Problems solved by technology

[0002] Test-tube virus-free propagation of grapes is the most effective biotechnology. Test-tube propagation of a virus-free or newly-bred variety and a rare grape variety can quickly provide a large number of neat and consistent virus-free grape seedlings in a short period of time, but test-tube propagation requires certain equipment and technical means, high cost (the cost of sugar-containing agar medium is 4-8 times that of conventional propagation) (see Cao Xiaodong, Cao Ziyi, 1996, cost and benefit analysis of plant test tube propagation

Plant Physiology Communications, 32(4), 284-291); it is easy to breed microorganisms on the sugar-containing agar medium, causing pollution; the roots of the test tube seedlings have no root hairs, and it is difficult to transplant (Table 6); the sugar-containing agar medium consumes a lot of electricity , much labor and high cost (see Table 5)

If a simple vermiculite medium with only inorganic nutrients is used, the bacteria cannot grow, the medium is simple, saves labor and electricity, and can greatly reduce the cost, but there is no organic component and auxin in the vermiculite medium , resulting in slower rooting of grape test-tube seedlings, adding penicillin can promote rooting

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