Process for biodegrading yellow water of white spirit factory

A technology of biodegradation and liquor, applied in the field of bioengineering, can solve the problems of cost increase

Inactive Publication Date: 2012-09-19
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ozone method does not need to add any chemicals, and there is no secondary pollution, but the cost will increase correspondingly when treating wastewater on a large scale

Method used

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  • Process for biodegrading yellow water of white spirit factory
  • Process for biodegrading yellow water of white spirit factory
  • Process for biodegrading yellow water of white spirit factory

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Production of bacteria on test tube slant

[0028] Insert a ring of Aspergillus oryzae (Aspergillus oryzae) into the newly configured potato sucrose medium ("Industrial Microbiology Experimental Technology Manual" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367) (China Common Microbial Cultures Preservation Management Center, CGMCC5992), 26°C, incubation time 50 hours; store at 4°C for later use.

[0029] 2. Production of cultured strains in liquid shake flasks

[0030] Accurately weigh 5 grams of glucose, 0.1 grams of ammonium tartrate, 0.2 grams of benzyl alcohol, 0.2 grams of magnesium sulfate, 0.5 grams of Tween 80, 2 grams of potassium dihydrogen phosphate, 3 grams of phthalic acid buffer, 1000 mL of water, pH 5 , divided into 250mL triangular flasks, each bottle 50mL, a total of 20 bottles, sterilized at 120 ° C for 40 minutes; after cooling, each bottle was inserted into a ring of Aspergillus oryzae strains preserved at 4 ° C, at 25 ° C, 150 rpm, cultu...

Embodiment 2

[0038] 1. Production of bacteria on test tube slant

[0039] Insert a ring of Aspergillus oryzae into the newly configured potato sucrose medium ("Industrial Microbiology Experimental Technology Manual" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367), at 30°C, and cultivate for 100 hours ; Store at 7°C for later use.

[0040] 2. Production of cultured strains in liquid shake flasks

[0041] Accurately weigh 150 grams of glucose, 250 grams of ammonium tartrate, 15 grams of benzyl alcohol, 0.4 grams of magnesium sulfate, 4 grams of Tween 80, 5 grams of potassium dihydrogen phosphate, 20 grams of phthalic acid buffer, 10 L of water, pH 6, Divide into 250mL triangular flasks, 100 grams per bottle, 100 bottles in total, sterilize at 130°C for 30 minutes; after cooling, insert a ring of Aspergillus oryzae strains preserved at 7°C, and cultivate 50 hours;

[0042] 3. Preparation of first-level liquid strains

[0043] Accurately weigh 2000 grams of glucose, 200 grams of ...

Embodiment 3

[0050] 1. Production of bacteria on test tube slant

[0051] Insert a ring of Aspergillus oryzae into the newly configured potato sucrose medium ("Industrial Microbiology Experimental Technology Manual" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367), at 35°C, and cultivate for 144 hours ; Store at 10°C for later use.

[0052] 2. Production of cultured strains in liquid shake flasks

[0053] Accurately weigh 300 grams of glucose, 50 grams of ammonium tartrate, 30 grams of benzyl alcohol, 10 grams of magnesium sulfate, 100 grams of Tween 80, 90 grams of potassium dihydrogen phosphate, 180 grams of phthalic acid buffer, 10 L of water, pH7.5 , divided into 250mL triangular flasks, each bottle 120 mL, a total of 80 bottles, sterilized at 140 ° C for 20 minutes; after cooling, each bottle was inserted into a ring of Aspergillus oryzae strains stored at 10 ° C, at 35 ° C, 150 rpm, Cultivate for 72 hours;

[0054] 3. Preparation of first-level liquid strains

[0055] Ac...

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Abstract

The invention discloses a process for biodegrading yellow water of a white spirit factory, and relates to the technical field of bioengineering. According to the process, aspergillus oryzae is utilized as a starting strain, and the COD (Chemical Oxygen Demand) of the yellow water of the spirit factory is reduced through a biological fermentation process. The process comprises the flow consisting of the following steps of: inoculating the aspergillus oryzae; performing shake-flask culture and first-stage seed culture; inoculating into diluted yellow water of the white spirit factory; and fermenting and culturing. By using the method, the COD removal rate can reach 60-98 percent, the BOD (Biochemical Oxygen Demand) removal rate reaches 90-99 percent, the pH is increased from original 3.5 to 7.0-8.0, the produced wastewater is subjected to centrifugation and molecular interception, and thalli and active molecules serving as feed additives can be obtained.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to using Aspergillus oryzae to degrade winery yellow water. Dilute the original winery yellow water by 3 to 10 times and insert Aspergillus oryzae species to degrade COD in wastewater. After Aspergillus oryzae is degraded, the COD removal rate of the wastewater can reach 60-98%, the BOD removal rate can reach 90-99%, and the pH rises from the original pH to 7.0. At the same time, the mycelium and active components can be extracted from the degraded wastewater to be used as feed additive. Background technique [0002] Liquor has a traditional history of more than one thousand years in China. In recent years, with the rapid development of the liquor industry, the environmental problems it brings are becoming increasingly serious. Although my country has been exploring and treating liquor production wastewater for a certain number of years, the overall situation is not optimis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/14C12R1/69C02F103/32
Inventor 张志才任晓锋刘丹申王莉
Owner JIANGSU UNIV
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