Microbial organic fertilizer with moisture and fertility retention functions and preparation method of microbial organic fertilizer
A bio-organic fertilizer, water and fertilizer retention technology, applied in fertilizer mixture, application, fertilization device and other directions, can solve problems such as failure to play the expected role, water and fertilizer evaporation, waste, etc., to reduce the number of top-dressing and the amount of top-dressing, The effect of enhancing disease resistance and reducing labor intensity
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Embodiment 1
[0021] The cultivation of embodiment 1 bacterial classification
[0022] 1) Preparation of nutrient broth agar seed medium: 100g of peptone, 30g of beef extract, 50g of NaCl, 10L of distilled water, pH7.0, sterilized at 121°C for 15min, cooled for later use.
[0023] Preparation of seed solution: Inoculate Bacillus subtilis into the seed medium and culture at 37° C. for 8-12 hours to obtain seed solution; Bacillus subtilis was purchased from Institute of Microbiology, Chinese Academy of Sciences, number 1.1086.
[0024] 2) Preparation of seed medium: weigh 500g of glutamic acid, 500g of glucose, and 9000g of water; first add a small amount of water to glutamic acid and glucose to dissolve them, and then add the remaining water; sterilize at 115°C for 20 minutes, Let cool and set aside.
[0025] Preparation of seed solution: Inoculate Bacillus Nongguan No. 1 into the seed medium, and culture at 40-60° C. for 8-12 hours to obtain seed solution. Nongguan No. 1 Bacillus is a str...
Embodiment 2
[0028] Select any seed solution cultivated above, and spray it evenly into the material according to 1-3% of the weight of the material to be fermented, with a carbon-to-nitrogen ratio (C / N) of 20-30, and adjust the initial water content between 40-60% , mixed evenly, cultivated at 60-70°C for 15-30 days, turned over every 7 days, and the finished product was obtained after fermentation.
[0029] The material to be fermented is one or more of human and animal manure, household waste or plant straw; compost fermentation can be used or related fermentation equipment can be used for fermentation. If compost fermentation is used, the heat generated by the growth of microorganisms is used to maintain the temperature. The temperature difference between winter and summer is large, the fermentation time required in summer is short, and the fermentation time is longer in winter; and the components or component ratios of the materials to be fermented are different, and the required ferme...
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