Diagnostic kit and application of BFAR (bifunctional apoptosis regulator) in preparation of gastric cancer early diagnostic reagent

A diagnostic kit and gastric cancer technology, applied in the field of biological sciences, can solve problems such as the inability to achieve high sensitivity and high specificity in the diagnosis of gastric cancer, and achieve the effect of easy operation

Active Publication Date: 2012-09-19
SHANGHAI JIAO TONG UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

[0008] The first object of the present invention is to provide a diagnostic kit to solve the technical problem that the gastric cancer diagnostic kits in the prior art cannot realize early diagnosis of gastric cancer with high sensitivity and high specificity
[0009] The second object of the present invention is to provide an application of BFAR in the preparation of early diagnosis reagents for gastric cancer, so as to solve the technical problem that the diagnostic reagents for gastric cancer in the prior art cannot realize the early diagnosis of gastric cancer with high sensitivity and high specificity

Method used

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  • Diagnostic kit and application of BFAR (bifunctional apoptosis regulator) in preparation of gastric cancer early diagnostic reagent
  • Diagnostic kit and application of BFAR (bifunctional apoptosis regulator) in preparation of gastric cancer early diagnostic reagent
  • Diagnostic kit and application of BFAR (bifunctional apoptosis regulator) in preparation of gastric cancer early diagnostic reagent

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Embodiment Construction

[0038] The present invention will be further described below in conjunction with the embodiments and the accompanying drawings.

[0039] 1. Expression, purification and identification of BFAR:

[0040] The protein BFAR is obtained by overexpression induced by genetically engineered Saccharomyces cerevisiae using galactose, and then separated and purified by agarose affinity medium (glutathione). The results of silver staining and Western-Blotting identification Respectively as figure 1 with figure 2 shown.

[0041] 2. Preparation of serum samples:

[0042] Centrifuge the whole blood sample at 1000g for about 20 minutes after standing at room temperature for 2 hours or overnight at 4°C, and take the supernatant for immediate detection; or aliquot and store the sample at -20°C or -80°C, but avoid Freeze and thaw repeatedly. Thawed samples should be centrifuged again prior to testing. The tested samples cannot contain NaN 3 , because NaN 3 Inhibits horseradish peroxidas...

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Abstract

The invention relates to the field of bioscience and particularly relates to a diagnostic kit and application of BFAR (bifunctional apoptosis regulator) in the preparation of a gastric cancer early diagnostic reagent. The diagnostic kit is used for gastric cancer early diagnosis and comprises an enzyme label plate, human protein BFAR, standard serum, an enzyme label reagent, enzyme substrate solution, sealing liquid, sample diluent, washing liquid and stop solution, wherein the human protein BFAR is coated on the enzyme label plate. Compared with the prior art, the diagnostic kit has the following advantages: 1. a commercialized kit which is sensitive, safe, reliable and easy to operate is provided, can be used for qualitative determination of the level of IgA antibody resisting against the protein BFAR in human serum and is beneficial to assistance in the early diagnosis of gastric cancer; and 2. the provided serum biomarker has human protein BFAR specificity being 84%, and the sensitivity being 85%, thus having the advantages of high specificity and high sensitivity.

Description

technical field [0001] The invention relates to the field of biological sciences, in particular to a diagnostic kit and the application of BFAR in the preparation of early-stage diagnostic reagents for gastric cancer. Background technique [0002] Gastric cancer is a common malignant tumor that endangers human health. According to the statistics of the World Health Organization (WHO) in 2008 (World Health Organization Annual Statistics, 2008), the death rate of gastric cancer patients ranks second in the world. In 2006 WHO statistics showed nearly 950,000 new cases and about 700,000 deaths from this disease. In my country, there are more than 300,000 new gastric cancer patients each year, and about 160,000 people die from gastric cancer. The mortality rate ranks first among malignant tumors, which seriously threatens people's lives and health (Chen Zhu, the third national retrospective sampling survey report on causes of death, 2008) . In order to reduce the mortality rate ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/96
Inventor 杨丽娜陶生策刘炳亚王靖方郭书娟朱正纲李建芳燕敏
Owner SHANGHAI JIAO TONG UNIV
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