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New application of pterostilbene to anti-fungal biofilm

A biofilm, red sandalwood stilbene technology, applied in the field of medicine, can solve problems such as unseen red sandalwood and stilbene

Inactive Publication Date: 2012-10-03
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] We found that pterostilbene alone can effectively inhibit the growth and proliferation of fungal biofilms in vitro, and has a good inhibitory effect on the formed fungal biofilms. So far, there has been no report on the antifungal biofilms of pterostilbene alone.

Method used

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  • New application of pterostilbene to anti-fungal biofilm
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  • New application of pterostilbene to anti-fungal biofilm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Effects of Pterostilbene alone on biofilms of different Candida albicans strains.

[0015] Materials and methods

[0016] 1. Test drug

[0017] Pterostilbene: provided by Guangzhou Chemical Co., Ltd., Chinese Academy of Sciences.

[0018] Fluconazole: purchased from Sigma Company.

[0019] Amphotericin B: purchased from Sangon Bioengineering Co., Ltd.

[0020] XTT: purchased from Sigma Corporation.

[0021] Menadione (menadione): purchased from Sigma Company.

[0022] Dimethyl sulfoxide (DMSO): China Pharmaceutical (Group) Shanghai Chemical Reagent Company, redistilled before use.

[0023] Acetone: purchased from Sigma Company.

[0024] Pterostilbene was made into a 6.4 mg / ml solution with dimethyl sulfoxide, amphotericin B was made into a 4 mg / ml solution with dimethyl sulfoxide, and fluconazole was made into a 102.4 mg / ml solution with dimethyl sulfoxide. Store at -20°C. Before the experiment, the drug stock solution was taken out and melted in a 3...

Embodiment 2

[0056] Embodiment 2: The effect of pterostilbene alone on the biofilm of different Cryptococcus neoformans strains

[0057] Materials and methods

[0058] 1. Test drug

[0059] Pterostilbene: provided by Guangzhou Chemical Co., Ltd., Chinese Academy of Sciences.

[0060] Fluconazole: purchased from Sigma Company.

[0061] Amphotericin B: purchased from Sangon Bioengineering Co., Ltd.

[0062] XTT: purchased from Sigma Corporation.

[0063] Menadione (menadione): purchased from Sigma Company.

[0064] Dimethyl sulfoxide (DMSO): China Pharmaceutical (Group) Shanghai Chemical Reagent Company, redistilled before use.

[0065] Acetone: purchased from Sigma Company.

[0066] Pterostilbene was made into 6.4 mg / ml with dimethyl sulfoxide, amphotericin B was made into 4 mg / ml with dimethyl sulfoxide, and fluconazole was made into 102.4 mg / ml with dimethyl sulfoxide. Store at ℃. Before the experiment, the drug stock solution was taken out and melted in a 35°C incubator, mixed t...

Embodiment 3

[0097] Embodiment 3: Pterostilbene single use is to the effect of different Aspergillus strain biofilms

[0098] Materials and methods

[0099] 1. Test drug

[0100] Pterostilbene: provided by Guangzhou Chemical Co., Ltd., Chinese Academy of Sciences.

[0101] Fluconazole: purchased from Sigma Company.

[0102] Amphotericin B: purchased from Sangon Bioengineering Co., Ltd.

[0103] XTT: purchased from Sigma Corporation.

[0104] Menadione (menadione): purchased from Sigma Company.

[0105] Dimethyl sulfoxide (DMSO): China Pharmaceutical (Group) Shanghai Chemical Reagent Company, redistilled before use.

[0106] Acetone: purchased from Sigma Company.

[0107] Pterostilbene was made into 6.4 mg / ml with dimethyl sulfoxide, amphotericin B was made into 4 mg / ml with dimethyl sulfoxide, and fluconazole was made into 102.4 mg / ml with dimethyl sulfoxide. Store at ℃. Before the experiment, the drug stock solution was taken out and melted in a 35°C incubator, mixed thoroughly, ...

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Abstract

The invention discloses new application of the pterostilbene to the anti-fungal biofilm and relates to the application of the pterostilbene to the anti-fungal biofilm therapeutic. The pterostilbene as a compound has the follow chemical structural formula. The experiments prove that the pterostilbene has good anti-fungal biofilm activity in vitro. The experimental results prove that the pterostilbene can effectively inhibit the growth and the proliferation of the fungal biofilm in vitro and can effectively inhibit the mature biofilm.

Description

Technical field: [0001] The invention relates to the technical field of medicine, and relates to a new application of pterostilbene in antifungal biofilms. Background technique: [0002] Pterostilbene is derived from the antifungal active ingredient in dried blood products. It belongs to polyhydroxy stilbene compounds and is a homologue of resveratrol. Its structure is shown in formula (I). It is a white crystalline powder and is sensitive to air. , with a melting point of 89-92°C, has rich medicinal value, and has a certain effect on the treatment of cancer, hypertension, and hyperlipidemia. Tu Pengfei, Peking University Modern Research Center of Traditional Chinese Medicine, etc. have reported on the antifungal properties of pterocarpus stilbene (Hu Yingqing, Tu Pengfei, Research on stilbene compounds and their antifungal activities in Dracaena saponifera, Chinese herbal medicine, 2001, 32 (2): 104 -105), but there is no report on the antifungal biofilm of pterostilbene a...

Claims

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Application Information

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IPC IPC(8): A01N31/16A01P3/00
Inventor 王彦赵兰雪李德东邹永古金华曹永兵姜远英
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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