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Method for comprehensively utilizing desert parasitic plant cynomorium and product and application thereof

A technology of Cynomorium, plant material, applied in the field of Cynomorium polyphenol-cysteine ​​condensate and its composition, Cynomorium triterpene component extract field, can solve the problem of Cynomorium polyphenol and cysteine ​​condensate Preparation and application have not yet been reported and other issues

Active Publication Date: 2012-10-03
INNER MONGOLIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Cynomorium contains a large amount of polyphenolic compounds, but the preparation and application of Cynomorium polyphenols and cysteine ​​condensation products have not been reported yet

Method used

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  • Method for comprehensively utilizing desert parasitic plant cynomorium and product and application thereof
  • Method for comprehensively utilizing desert parasitic plant cynomorium and product and application thereof
  • Method for comprehensively utilizing desert parasitic plant cynomorium and product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Cynomorium triterpenoids extract and Cynomorium low molecular weight tannin-cysteine ​​condensation preparation

[0050] Cynomorium cynomorium (purchased from Hohhot Jingyuanda Pharmacy in June 2011, 2 grams) was crushed and extracted by adding 10 ml of 95% ethanol and shaking at room temperature for 12 hours, filtered, and the filtrate was detected by LC-MS and compared with the standard substance to confirm that it contained cynomorium AHA (22.7 μg / ml), Acetylursolic Acid (8.5 μg / ml) and Ursolic Acid Malonate (63.5 μg / ml). The filtrate was concentrated to dryness to obtain 128.6 mg of ethanol extract containing Cynomorium triterpenoids as off-white solid.

[0051] If 95% ethanol is changed into 40% ethanol, the extract treated with the same method contains ursolic acid (1.0 microgram / ml), acetyl ursolic acid (0.0 microgram / ml) and ursolic acid malonate half ester (10.8 micrograms / ml). The filtrate was concentrated to dryness to obtain 943.9 mg of brow...

Embodiment 2

[0061] Example 2: Separation and identification of Cynomorium low molecular weight tannin-cysteine ​​condensates 6, 7 and 8

[0062] With reference to the method of Example 1, 200 grams of Cynomorium cynomorium was extracted with 1000 milliliters of 95% ethanol by shaking at room temperature for 12 hours. After filtering, the filter residue was prepared by "A) reacting with cysteine ​​hydrochloride at 60°C" in Example 1. According to the method described above, the resulting filtrate was subjected to ODS column chromatography and water-methanol gradient elution to obtain Cynomorium low molecular weight tannin-cysteine ​​condensates 6, 7 and 8. Bruker Avance III 500NMR analyzer ( 1 H NMR: 500MHz; 13 C NMR: 125MHz) measure the NMR data as follows:

[0063] Compound 6:

[0064] 1 H NMR (CD 3 OD) δ2.98 (dd, 1H, J=9.5, 14.5Hz, cys3), 3.62 (br d, 1H, J=14.5Hz, cys3), 4.01 (dd, 1H, J=4.0, 9.5Hz, 3) , 4.14 (dd, 1H, J = 4.0, 9.5Hz, cys2), 4.25 (d, 1H, J = 4.0Hz, 4), 4.77 (overl...

Embodiment 3

[0072] Example 3: Determination of antioxidant activity of Cynomorium low molecular weight tannin-cysteine ​​condensate of the present invention

[0073] Using the method reported by the inventors (Ma CM, et al., "Daneshtalab M.Synthesis, anti-HIV and anti-oxidant activities of caffeoyl 5,6-anhydroquinic acid derivatives", Bioorg Med Chem 2010, 18: 863-869 ), compared the free radical scavenging effect of Cynomorium low molecular weight tannin-cysteine ​​condensate of the present invention and (epi)catechins themselves.

[0074] The free radical scavenging effect was measured in 96-well plates. Add 10 μl test compound solution (DMSO, 1 mg / ml, 0.5 mg / ml, 0.25 mg / ml, 0.125 mg / ml,) and 190 μl DPPH[1,1-diphenyl-2-trinitrophenylhydrazine to each well (1,1-Diphenyl-2-picrylhydrazyl radical)] in (0.1 mM) ethanol solution. Absorbance A at 540 nm was measured after incubation at room temperature for 20 minutes. The formula for calculating the effect of scavenging free radicals is ...

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Abstract

The invention relates to a method for comprehensively utilizing desert parasitic plant cynomorium and a product and application thereof and particularly relates to a cynomorium triterpene component extract and a cynomorium polyphenol-cysteine condensation compound and a composition of the cynomorium triterpene component extract and the cynomorium polyphenol-cysteine condensation compound, a preparation method thereof and application thereof in aspects of drugs, (functional) foods, cosmetics and the like.

Description

technical field [0001] The present invention relates to the comprehensive utilization method of the desert parasitic plant Cynomorium cynomorium and its products and their applications, in particular to the extract of Cynomorium cynomorium triterpenoids, Cynomorium polyphenol-cysteine ​​condensate and their composition, their preparation method, and their applications in pharmaceuticals, (functional) foods, cosmetics, etc. Background technique [0002] Cynomorium songaricum (Latin name: Cynomorium songaricum) is a parasitic plant with the same source of medicine and food in the desert area of ​​northwest my country. One of the common names is elixir, which has the effect of strengthening the body. [0003] Studies have shown that ursolic acid and acetylursolic acid are contained in the fleshy stem of Cynomorium [Zhang Siju, Zhang Shuyun. "Research on the Chemical Composition of Chinese Medicine Cynomorium", Chinese Journal of Pharmaceutical Sciences, 26, 649-651 (1991)]; Ur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K8/97A61P39/06A61P35/00A61P25/28A61P5/50A61P3/10A61P3/04A61P1/16A61P31/12A23L1/29A61K125/00A23L33/00
Inventor 马超美蒙昊聪张义文
Owner INNER MONGOLIA UNIVERSITY
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