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Method for extracting natural taurine from nori

A technology of taurine and seaweed, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of not meeting market demand, long growth cycle, and few sources, and achieves production conditions that are easy to control and grow The effect of short cycle time and low production cost

Inactive Publication Date: 2014-04-30
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these seafood products have a long growth cycle and few sources, which cannot meet market demand.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Crush laver into powder, pass through a 200-mesh sieve, weigh 25 g, add 0.02 mol / L HCl in the extract according to the ratio of solid to liquid 1:40, bathe in 80°C water for 10 h, and filter with filter cloth. The pH of the filtrate was adjusted to 3 with HCl, the protein was precipitated, centrifuged, and the supernatant was taken. Then adjust the pH to 10 with NaOH, precipitate the protein, centrifuge, and take the supernatant. There is 470 μg / ml of taurine in the supernatant. Use a rotary evaporator to concentrate the sample solution to 1 / 3 of the volume of the original solution, pass through a 0.45 μm microporous membrane, and set aside. The concentrated laver sample solution was adjusted to pH 4.5, desalted and purified with cation exchange resin at normal temperature and pressure, and eluted with deionized water. Collect the taurine solution. The collected taurine solution was placed in a refrigerator at 4° C. for 2 hours. After a small amount of taurine crysta...

Embodiment 2

[0023] Crush laver into powder, pass through a 140-mesh sieve, weigh 25g, add distilled water according to the ratio of solid to liquid 1:45, bathe in 80°C water for 8h, and filter with filter cloth. The filtrate was centrifuged after acid-base precipitation, and there was 400 μg / ml of taurine in the supernatant. Adjust the pH of the laver sample liquid concentrated to 1 / 4 volume of the original liquid to 4.8, and use a cation exchange resin to desalt and purify at normal temperature and pressure. The separation, purification and crystallization steps are the same as in Example 1. The precipitated amount of taurine crystals was 0.18 g.

Embodiment 3

[0025] Crush laver into powder, pass through a 140-mesh sieve, weigh 25g, add distilled water according to the ratio of material to liquid 1:40, bathe in water at 80°C for 6 hours, and filter with gauze; continue to add distilled water to extract the filter residue according to the ratio of material to liquid 1:20 for 6 hours, Strain through a filter cloth. The filtrates were combined, and the filtrate was centrifuged after acid-base precipitation, and there was 290 μg / ml of taurine in the supernatant. Adjust the pH of the laver sample liquid concentrated to 1 / 5 of the volume of the original liquid to 4.8, and use a cation exchange resin to desalt and purify at normal temperature and pressure. The separation, purification and crystallization steps are the same as in Example 1. The precipitated amount of taurine crystals was 0.20 g.

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PUM

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Abstract

The invention relates to a method for extracting natural taurine from nori, and belongs to the technical field of deep-processing of marine products. The method is characterized by comprising the following steps: extracting nori which is taken as the main raw material, then, removing protein and polysaccharide by a precipitation method, desalting and purifying taurine with a cation exchange resin, and finally, crystallizing the taurine with ethanol to obtain the natural taurine. The natural taurine prepared by the method is safe and nontoxic; the extraction method is simple and feasible and has low requirements on the equipment; and the yield of the taurine is 0.60 to 0.92 g / 100g.

Description

1. Technical field [0001] The invention relates to a method for extracting natural taurine from laver, belonging to the technical field of deep processing of seafood. 2. Background technology [0002] Taurine, also known as taurine, taurine, taurin, and 2-aminoethanesulfonic acid, has a chemical structure of H 2 N-CH 2 -CH 2 -SO 3 H, has functions such as maintaining vision. Studies have shown that taurine is a biologically active substance that regulates the normal physiological functions of the body, and plays an important role in regulating the central nervous system, digestion, reproduction and immune systems. With the in-depth research on the biological functions of taurine, taurine products are gradually recognized by people, showing a broad market prospect. [0003] At present, the production methods of taurine mainly include artificial synthesis and biological extraction. The yield of artificially synthesized taurine is relatively high, but the raw materials ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C309/14C07C303/44
Inventor 顾振新张婷韩永斌
Owner NANJING AGRICULTURAL UNIVERSITY
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