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Method for preparing high-purity soybean oligosaccharide from soybean whey wastewater

A technology of soybean whey wastewater and soybean oligosaccharides, which is applied to the preparation of oligosaccharides and sugar derivatives, chemical instruments and methods, etc., can solve the problem of reducing the health care effect, the membrane separation effect cannot reach the best level, and it is unfavorable for diabetics Food and other problems, to achieve the effect of short processing time

Active Publication Date: 2015-01-28
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the degree of polymerization of sucrose is similar to that of raffinose, the membrane separation effect cannot reach the optimum level. The content of functional components (raffinose, stachyose) in soybean oligosaccharides sold on the market is 60%, and there are more sucrose The presence of some simple sugars reduces the health effect and is not conducive to the consumption of diabetics

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 100L of whey wastewater obtained from the production of soybean protein has a pH value of 5.4. Soybean whey wastewater is pre-clarified and filtered using a stainless steel microfiltration membrane with a membrane pore size of 20 nm. The treatment temperature is controlled at 60 ° C and the operating pressure is 4 bar; 0.01% (w / v) sucrase is added to the ultrafiltration filtrate to stir the reaction , pH3, reaction temperature 40°C, reaction time 12h. The enzyme inactivating solution is re-filtered by an ultrafiltration membrane with a molecular weight cut-off of 3000Da to remove enzymes, and then filtered and separated through a nanofiltration membrane with a molecular weight cut-off of 150Da. The pure concentration mode or diafiltration mode is adopted, the filtration temperature is 50°C, and the operating pressure TMP is 35 ~40bar, the nanofiltration concentrated solution is concentrated under reduced pressure and freeze-dried to obtain soybean oligosaccharide powder...

Embodiment 2

[0027] Using molasses 40L, after diluting 6 times, the pH value of the molasses system is about 5.3-5.5. Use ceramic membrane microfiltration with a membrane pore size of 100nm, and then use a 5000Da ultrafiltration membrane to pre-clarify and filter soybean whey wastewater. The treatment temperatures are 80 and 30°C, and the operating pressures are 2.5bar and 1bar respectively; Add 0.02% and 0.15% (w / v) cellulase and sucrase respectively to the filtrate and stir for reaction, pH 4.5, reaction temperature 30°C, reaction time 2h to inactivate the enzyme solution and refilter through ultrafiltration membrane with molecular weight cut off 3000Da After removing the enzyme, it is filtered and separated through a nanofiltration membrane with a molecular weight cut-off of 250Da. The simple concentration mode or diafiltration mode is adopted. The filtration temperature is 55°C and the operating pressure TMP is 40bar. Soybean oligosaccharide powder with a purity of 98%.

Embodiment 3

[0029]Sequentially use a microfiltration membrane with a membrane pore size of 200nm and an ultrafiltration membrane with a molecular weight cutoff of 3000Da to pre-clarify and filter soybean whey wastewater. The treatment temperature is 25°C and the operating pressure is 2bar; add 1% (w / v) to the filtrate glucosidase, fructofuranosidase, fructosyltransferase compound enzyme preparation stirring reaction, pH 6 ~ 7, reaction temperature 40 ~ 45 ℃, reaction time 4h, inactivate the enzyme solution through the ultrafiltration membrane with a molecular weight cut-off of 3000Da to re-filter the enzyme Afterwards, it is filtered and separated through a nanofiltration membrane with a molecular weight cut-off of 350Da. The simple concentration mode or diafiltration mode is adopted, the filtration temperature is 60°C, and the operating pressure TMP is 15bar. Soybean oligosaccharide powder with a polysaccharide purity of 97.5%.

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Abstract

The invention relates to a method for preparing a high-purity soybean oligosaccharide from soybean whey wastewater, which comprises the following steps: (1) preprocessing of raw materials: heating soybean whey wastewater or soybean molasses diluent obtained from an industrial production in water bath to 10 to 80 DEG C, and pre-clarifying and filtering the heated product with a microfiltration membrane and / or an ultra-filtration membrane; (2) an enzymolysis process: adding enzyme into the filtrate of the soybean whey wastewater or the soybean molasses diluent obtained in step (1), stirring the obtained product at a speed of 50 rpm for 1 to 3 min, regulating the pH value to be 3 to 11, reacting the obtained product at the temperature of 0 to 60 DEG C for 10 min to 12 h, and then, inactivating the obtained product in boiling water bath for 10 to 15 min to obtain enzyme-inactivated solution; and (3),a nanofiltration and separation process: filtering the enzyme-inactivated solution with an ultra-filtration membrane again to remove enzyme, and then filtering and separating the obtained solution with a nanofiltration membrane to obtain nanofiltration concentrated solution, which is oligosaccharide solution. Compared with the prior art, the total amount of cane sugar, fructose, glucose and other monosaccharide in the prepared product does not exceed 5%, so that the technology is suitable for industrial mass production.

Description

technical field [0001] The invention relates to a method for preparing high-purity soybean oligosaccharides from soybean whey wastewater, in particular to a method for preparing high-purity soybean oligosaccharides from soybean whey wastewater by enzymatic hydrolysis reaction coupling membrane separation technology, belonging to The technical field of comprehensive utilization of food processing waste. Background technique [0002] Soybean oligosaccharides refer to those naturally occurring in soybeans, with raffinose, stachyose and verbascose as the main functional components. Soybean oligosaccharides can promote the reproduction of bifidobacteria in the human intestinal tract, improve the distribution of intestinal flora, and enhance the body's ability to resist diseases; at the same time, it does not generate blood sugar, and can be used as a health food for diabetics and obese patients; and reduce the burden on the liver, Reduce blood lipids, enhance immunity, prevent c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H3/06C07H1/08
Inventor 赵黎明归志雯陈超琴蒋丽华周家春夏泉鸣赵鹤飞邱勇隽
Owner EAST CHINA UNIV OF SCI & TECH
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