Application of fresh euphorbia helioscopia juice in preparation of medicine for treating herpes simplex virus
The technology of herpes simplex virus and varnish is applied in the application field of preparing medicine for treating herpes simplex virus disease, and achieves the effects of low adverse reaction, small effective dose and strong anti-herpes simplex virus activity
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Embodiment 1
[0022] Preparation of Xianze lacquer juice: take fresh lacquer, remove the roots, wash, mash the fresh stems, leaves and flowers, filter and squeeze the juice through gauze to obtain fresh juice containing fresh crude drug 18g / ml, filter and sterilize at -20°C Save it for later use.
Embodiment 2
[0023] Embodiment 2 Pharmacological experiment
[0024] 1 material
[0025] 1.1 Cell line Vero cells (African green monkey kidney cells) were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences, passaged and preserved in our laboratory.
[0026] 1.2 Drugs. Fresh lacquer juice prepared in Example 1 of the present invention, lacquer water decoction (the preparation method is: take the dried lacquer, add 10 times the amount of distilled water and decoct it to half the volume at 100°C, filter to obtain the filtrate, and concentrate to Extract, so that the crude drug content is 20mg / ml, stored at 4°C); the positive control drug is acyclovir (ACV), Hubei Qianjiang Pharmaceutical Co., Ltd., batch number 20040802.
[0027] 1.3 The main reagents are penicillin (Shandong Lukang), streptomycin sulfate (Amresco), MTT (thiazole salt, Genebase), trypsin (Nanjing Shengxing), EDTA (Sigma), RPMI-1640 medium (GIBCO), iodide Propidium (PI, Sigma, USA), RPMI-1640 (GIBCO), ...
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