Two-step enzyme measuring method and measuring reagent for creatinine in blood serum

A determination method and two-step enzyme technology, which are applied in the field of medical testing, can solve the problems of poor accuracy and precision of reagents, poor repeatability of clinical results, and inability to meet the requirements of routine testing.

Active Publication Date: 2012-10-10
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The endogenous creatine and the creatine produced by the hydrolysis of Cr show color successively. The instrument takes the quinone imine produced in the first step as a blank, and only calculates the content of creatine based on the quinone imine produced in the second step, so as to remove However, under normal circumstances, the absorption signal produced by endogenous creatine is low and unstable, so the reagent accuracy and precision of this method are poor, and the reproducibility of clinical results is not good, which cannot meet the requirements. Routine Testing Requirements

Method used

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  • Two-step enzyme measuring method and measuring reagent for creatinine in blood serum
  • Two-step enzyme measuring method and measuring reagent for creatinine in blood serum
  • Two-step enzyme measuring method and measuring reagent for creatinine in blood serum

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Effect test

Embodiment 1

[0037] Reagent 1:

[0038] Reagent 2:

[0039] The preparation method of Reagent 1 and Reagent 2 is a conventional method, that is, the components of Reagent 1 and Reagent 2 are added to distilled water and then mixed and stirred evenly.

Embodiment 2

[0041] Reagent 1:

[0042] Reagent 2:

[0043] The preparation method of embodiment 2 reagent is the same as embodiment 1.

Embodiment 3

[0045] Reagent 1:

[0046] Reagent 2:

[0047]

[0048] The preparation method of embodiment 3 reagent is the same as embodiment 1.

[0049] The test conditions for the determination of Cr in the sample by the reagent of the present invention are as follows: temperature: 37° C.; optical path of the cuvette is 1.0 cm. The main detection wavelength is 546nm, and the secondary wavelength is 700nm.

[0050] The method of measuring Cr in the sample by using the Cr determination reagent of the present invention is as follows: add R1 to the sample (calibration tube as the sample) and mix evenly, add R2 and mix evenly after incubating at 37°C for 5 minutes, record the absorbance A1, and react at 37°C for 5 minutes, Record the absorbance A2. The sample volume is 8 μl, the reagent 1 volume is 200 μl, and the reagent 2 volume is 100 μl.

[0051] The Cr content in the reagent of the present invention measures sample is calculated by the following formula:

[0052]

[0053] Th...

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Abstract

The invention discloses a two-step enzyme measuring method and measuring reagents for creatinine in blood serum. The hydrogen peroxide generated by endogenous creatine is eliminated by utilizing catalase in a reagent 1 of the creatinine measuring reagents, and the catalase in the reagent 1 is suppressed by utilizing the catalase in a reagent 2 of the creatinine measuring reagents, so that the creatinine content in a sample is measured. The invention has the advantages that the phenomenon that the cost is increased because of oxidizing hydrogen peroxide by the catalase, so that the detection result is low in accuracy and precision is avoided.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a two-step enzymatic assay method for creatinine in serum and a assay reagent. Background technique [0002] Creatinine (Cr) is a low molecular nitrogen compound with a relative molecular mass of 116,000. Cr is mainly produced by muscle and is the terminal metabolite of creatine. Under normal circumstances, the content of Cr in the human body is basically stable, generally maintained at 3~14mg / L, and it is only excreted by glomeruli and not reabsorbed by renal tubules. Since Cr is a small molecular substance that does not bind to plasma proteins, the measurement of serum Cr concentration and the Cr clearance rate calculated based on serum Cr and uric anhydride concentrations are simple and reliable indicators of glomerular filtration function, and are highly valued in clinical practice. [0003] Clinical laboratories usually use enzymatic method or alkaline picric acid m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 邹炳德邹继华沃燕波
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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