Chemical dyeing method for H2S in plant tissue
A technology of plant tissue and histochemistry, which is applied in the field of chemical staining of H2S in plant tissue, can solve the problems of high requirements for experimental conditions and high application cost of fluorescent probes, and achieve the effect of low cost and easy operation
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Embodiment 1
[0020] Take 3 Arabidopsis leaves grown for 6 weeks and soak in CuSO 4 In the saturated solution, take out the leaves after 6 hours, wash them with distilled water, boil them in 95% ethanol for 30 minutes to remove chlorophyll, take out the leaves and wash with distilled water and observe under a stereo microscope (see figure 1 ). CuS dark brown spots appear on the leaves, indicating the presence of H in plant tissues 2 S.
[0021] In order to further prove that the dark brown precipitate in the leaves is CuS, the leaf epidermis with dark brown spots was torn off to expose dense areas of dark brown precipitates, and X-ray photoelectron spectroscopy (XPS analysis) was performed after sufficient drying. It can be seen from the XPS analysis map that the leaves contain the most C, N, and O, which is consistent with the compositional characteristics of biological samples, and Cu and S can be detected, which is impossible in normal biological samples ( figure 2 A). A strong peak near 9...
Embodiment 2
[0023] Take 3 Arabidopsis leaves grown for 6 weeks and soak in CuSO 4 In the saturated solution, the leaves were taken out after 6 hours and washed with distilled water. The leaves were placed in a water bath of 95% ethanol at 60°C for 12 hours to remove chlorophyll. The leaves were taken out and washed with distilled water and observed under a stereo microscope. Dark brown spots appear on the leaves, indicating the presence of H in plant tissues 2 S.
Embodiment 3
[0025] In order to prove that the amount of CuS dark brown precipitation in the leaves is the same as the H in the leaves 2 The yield of S is correlated, and the following experiments are done:
[0026] The six-week-old Arabidopsis plants were immersed in a petri dish containing 30ml of 5%, 10%, 20%, and 40% PEG (simulated drought stress), respectively, and treated with 3 Arabidopsis plants in each treatment. After 12 hours, take out Arabidopsis thaliana, wash with distilled water, cut off the leaves of each treatment plant, divide them into two parts, and soak some leaves in CuSO 4 In saturated solution for 6 hours, a part of the leaves are used to determine H 2 S yield. Via CuSO 4 The leaves stained with saturated solution were washed with distilled water and boiled in 95% ethanol for 30 minutes to remove chlorophyll. The leaves were washed with distilled water and observed under a stereo microscope. image 3 . The negative control was the leaves that were chlorophyllized with ...
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