Metasequoia glyptostroboides bark extract, preparation method thereof and uses thereof

The technology of extract and Metasequoia is applied in the field of Metasequoia bark extract and its preparation, and achieves the effects of low cost, cardiovascular protection effect and simple operation.

Active Publication Date: 2012-10-17
ZHEJIANG ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the research on metasequoia bark

Method used

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  • Metasequoia glyptostroboides bark extract, preparation method thereof and uses thereof
  • Metasequoia glyptostroboides bark extract, preparation method thereof and uses thereof
  • Metasequoia glyptostroboides bark extract, preparation method thereof and uses thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Preparation of metasequoia bark extract

[0031] Extract 1kg of metasequoia bark powder (40 mesh) with 25kg of water at 50°C for 5 hours, filter off the residue to obtain 20L of extract, cool to 20°C, and filter. The filtrate was concentrated under reduced pressure at 40°C to 1 / 5 of the total volume of the filtrate to obtain a concentrated solution, which was extracted 3 times with ethyl acetate. The solution was dried with anhydrous sodium sulfate, then concentrated under reduced pressure to 1 / 5 of the total volume of the ethyl acetate extract to obtain a secondary concentrate, and poured into three times the volume of petroleum ether (boiling range 60℃-90℃). Let the precipitate stand, filter, dissolve the precipitate with ethyl acetate, precipitate and purify with petroleum ether (boiling range 60°C-90°C), wash with petroleum ether (boiling range 60°C-90°C), and dry under reduced pressure below 40°C to obtain Metasequoia bark extract, the sample name of the metasequ...

Embodiment 2

[0033] Preparation of metasequoia bark extract

[0034] 1 kg of metasequoia bark powder (60 mesh) was reflux extracted with 20 kg of 70% ethanol aqueous solution for 3 hours, and the residue was filtered to obtain 20 L of extract, which was cooled to 30 °C and filtered. Concentrate the filtrate under reduced pressure at 40°C until there is no alcohol smell, to obtain a concentrated solution, add water to 4L to suspend, and extract the suspension 3 times with ethyl acetate, each time the amount of ethyl acetate is 1 / 2 of the volume of the suspension , the combined ethyl acetate extracts were dried with anhydrous sodium sulfate, then concentrated under reduced pressure to 1 / 5 of the total volume of the ethyl acetate extracts to obtain a secondary concentrate, poured into three times the volume of the secondary concentrate under mechanical stirring Petroleum ether (boiling range 60°C-90°C). Let the precipitate stand, filter, dissolve the precipitate with ethyl acetate, precipita...

Embodiment 3

[0036] Preparation of metasequoia bark extract

[0037]1 kg of metasequoia bark powder (20 mesh) was reflux extracted with 20 kg of 95% ethanol aqueous solution for 2 hours, and the residue was filtered to obtain 20 L of extract, which was cooled to 25 °C and filtered. Concentrate the filtrate under reduced pressure at 40°C until there is no alcohol smell, to obtain a concentrated solution, add water to 4L to suspend, and extract the suspension 3 times with ethyl acetate, each time the amount of ethyl acetate is 1 / 2 of the volume of the suspension , the combined ethyl acetate extracts were dried with anhydrous sodium sulfate, then concentrated under reduced pressure to 1 / 5 of the total volume of the ethyl acetate extracts to obtain a secondary concentrate, poured into three times the volume of the secondary concentrate under mechanical stirring Petroleum ether (boiling range 60°C-90°C). Let the precipitate stand, filter, dissolve the precipitate with ethyl acetate, precipitat...

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Abstract

The invention discloses a Metasequoia glyptostroboides bark extract, a preparation method thereof, and uses thereof. The preparation method comprises the following steps: 1, extracting Metasequoia glyptostroboides bark powder in an extraction solvent at a temperature in a range from 25DEG C to a reflux temperature for 2-24h, and filtering out residues to obtain an extract solution; 2, cooling the extract solution, filtering, and concentrating the resulting filtrate to obtain a first concentrate; and 3, extracting the first concentrate with ethyl acetate, degreasing with petroleum ether or chloroform, and drying to obtain the Metasequoia glyptostroboides bark extract; or carrying out macroporous adsorption resin column chromatography on the first concentrate, collecting the resulting eluate, and concentrating and drying the eluate to obtain the Metasequoia glyptostroboides bark extract. The method has the advantages of simple operation, low cost, and no need of special devices, and is suitable for industrialized production. The Metasequoia glyptostroboides bark extract contains abundant proanthocyanidin and polyphenol components, and has a strong free radical removing effect, a strong antioxidation capability, a tyrosinase inhibition effect, an alpha-glucosidase inhibition effect and a cardiovascular protection effect.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to a metasequoia bark extract, a preparation method and application thereof. Background technique [0002] Metasequoia (Metasequoia glyptostroboides Hu et Cheng) is a single-species genus of Metasequoia in the family Firaceae. It is a unique relic species in my country and is called a "living fossil". The fossils of metasequoia were found to be widely distributed in the northern hemisphere during the Mesozoic Cretaceous and Cenozoic, but after the ice age of the fourth century, all other species of metasequoia became extinct. In the mid-1940s, Chinese botanists discovered metasequoia in local areas at the junction of Hubei, Sichuan and Hunan provinces. The mature cultivation and propagation technology of metasequoia makes metasequoia become the main landscaping tree species in China, and it has been introduced and cultivated in about 50 foreign countries and regions. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/13A61K8/97A61P39/06A61P17/00A61P9/00A61P3/10A61Q19/02A61Q19/08A23L1/29A61K129/00A23L33/00
Inventor 叶益萍李晓誉陈峰阳徐世芳
Owner ZHEJIANG ACAD OF MEDICAL SCI
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