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Preparation method and active part of pharmaceutical composition for preventing and treating acute coronary syndrome

A technology of active parts and compositions, applied in the field of a group of active parts obtained in the preparation method and preparation process, can solve the problems of troublesome decoction, difficulty in clinical drug development and new drug development, and large dosage

Active Publication Date: 2015-09-09
BEIJING UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the dosage of the original prescription is too large, it is troublesome to decoct, which brings difficulties to clinical medication and new drug development.

Method used

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  • Preparation method and active part of pharmaceutical composition for preventing and treating acute coronary syndrome
  • Preparation method and active part of pharmaceutical composition for preventing and treating acute coronary syndrome
  • Preparation method and active part of pharmaceutical composition for preventing and treating acute coronary syndrome

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0034] EXPERIMENTAL EXAMPLE 1 EXTRACTION PROCESS OPTIMIZATION

[0035] 1. Test method

[0036] (1) Extraction method: take by weighing about 10.8g of the four flavors of raw materials, and its weight ratio is honeysuckle: Scrophulariaceae: Angelica: licorice = 3: 3: 2: 1, and carry out according to the factors and levels selected in the orthogonal test table extract.

[0037] (2) Selection of extraction conditions: According to the existence characteristics of compounds in traditional Chinese medicine, water and ethanol are selected as extraction solvents, and the four factors of ethanol concentration, dosage, extraction time, and extraction times are investigated, and each factor is limited to three levels. See Table 1 for details.

[0038] Table 1 Factor level table

[0039] Level

A ethanol concentration (%)

BExtraction time (h)

C solid-liquid ratio

D extraction times

1

0

2

1∶6

2

2

55

3

...

experiment example 2

[0050] Inhibitory effect of the extract of Experimental Example 2 on the binding of NF-κB response elements

[0051] 1. Experimental steps

[0052] (1) Experimental materials: NF-κB-RE cells (pGL4.32[luc2P / NF-kappaB-RE]293A, Institute of Materia Medica, Chinese Academy of Medical Sciences), MEM-α medium (Gibco), fetal bovine serum (Gibco) , 96-well culture plate (Costar, 3599), Bright-Glo Luciferase luciferase detection reagent (Promaga), water-jacketed CO2 cell incubator (Sanyo, Japan), Safire2 high-speed multi-channel continuous wavelength microplate reader (TECAN, Austria ), EnSpire TM 2300 Multilabel Reader (PerkinElmer Corporation).

[0053] (2) Test samples:

[0054] Eight extracts obtained by the method described in Example 1 were provided by Beijing University of Traditional Chinese Medicine.

[0055] (3) Experimental steps:

[0056] Digest and count the logarithmically grown NF-κB-RE cells in the culture flask, and use 100ul system, 5×10 3 / well density inocula...

experiment example 3

[0067] The agonistic effect of experimental example three extracts on peroxisome proliferator receptor (PPARγ)

[0068] 1 material

[0069] 1.1 Reagents:

[0070] African green ape kidney cells (CV-1), PPRE plasmid, PPAR plasmid, RXRα plasmid, MEM-α medium (Gibco), fetal bovine serum (Gibco), Bright-Glo Luciferase luciferase detection reagent (Promega) , rosiglitazone.

[0071] 1.2 Instruments:

[0072] Water Jacketed CO 2 Cell incubator (Sanyo, Japan), EnSpireTM 2300MultilabelReader (PerkinElmer).

[0073] 2 methods

[0074] 2.1 Extraction of the test product

[0075] 8 extracts obtained by the method described in Example 1 are numbered 1 # -8 # , provided by Beijing University of Traditional Chinese Medicine.

[0076] 2.2 Cell Culture

[0077] African green ape kidney cells (CV-1) were cultured in DMEM medium supplemented with 10% FBS and 1% antibiotics (penicillin / streptomycin) in a 5% CO2 incubator at 37°C.

[0078] 2.3 Transfection and luciferase gene expressio...

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Abstract

The invention provides a method for extracting an active site from a traditional Chinese medicine composition. The method comprises the steps that: raw materials of honeysuckle flower, figwort, Chinese angelica and licorice are well mixed according to a weight ratio of 2.5-4:2.5-4:1.5-2.5:0.5-1.5; the mixture is extracted by using ethanol, such that an extraction liquid and residue are obtained; the extraction liquid is condensed and is loaded on macroporous resin; water, 30% ethanol, 60% ethanol, and 95% ethanol are respectively used as eluents for continuous eluting, such that a first extract, a second extract, a third extract and a fourth extract are respectively obtained; the residue is extracted by using water, such that a fifth extract is obtained; the fifth extract is loaded on macroporous resin; water, 55% ethanol and 95% ethanol are respectively adopted as eluents for continuous eluting, such that a sixth extract, a seventh extract and an eighth extract are respectively obtained. As results of experiments, the extracts 1-8 provided by the invention has preventing or treating effects against acute coronary syndrome. Also, the invention provides a set of active sites obtained in the preparation process.

Description

technical field [0001] The invention relates to a pharmaceutical composition for preventing and treating acute coronary syndrome, in particular to a preparation method of the modern dosage form of the composition and a group of active parts obtained during the preparation process. Background technique [0002] Acute coronary syndrome (ACS) is a group of clinical syndromes caused by acute myocardial ischemia, including acute myocardial infarction (AMI) and unstable angina (UA), which is a major and common disease that seriously endangers human health. Coagulation activation and vulnerable plaque inflammation play an important role in the occurrence of ACS and are two core pathological links. At present, anticoagulant and antiplatelet therapy have been deeply researched and widely used clinically; however, there is a lack of effective means in the treatment of controlling inflammation and stabilizing vulnerable plaques. [0003] Traditional Chinese medicine has shown great po...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/808A61P9/10
Inventor 陈立新吴圣贤聂波
Owner BEIJING UNIV OF CHINESE MEDICINE