Method for improving intestinal colony structure and application

A technology of intestinal flora and intestinal bacteria, applied in the application field of the process, can solve the problems of negative correlation between the host's health status and unclear intestinal flora

Inactive Publication Date: 2012-10-24
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, the mechanism of intestinal flora participating in the development of diseases is not very clear. Specifically, what kind of bacteria a

Method used

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  • Method for improving intestinal colony structure and application
  • Method for improving intestinal colony structure and application
  • Method for improving intestinal colony structure and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example Berberine significantly changed the intestinal flora structure of rats fed normal diet and high-fat diet

[0040] Using 454 pyrosequencing technology and multivariate statistical methods to analyze the intestinal flora structure of rats in each group. Using principal coordinate analysis (PCoA) based on the unweighted UniFrac distance, the results showed that whether rats were fed a normal diet or a high-fat diet, daily gavage of 100 mg / kg berberine could significantly change the intestinal flora. the whole frame. The effect of berberine could explain 12.6% of the overall variation in gut microbiota structure (see figure 1 A). There is also a significant effect of diet on the structure of intestinal flora, from figure 1 A It can be seen that the animal flora structure of the two different diets is significantly different in the direction of principal coordinate 3 (3.7%). Multivariate analysis of variance (MANOVA) was used to statistically analyze the differen...

Embodiment 2

[0041] Example 2 Berberine significantly enriched the content of short-chain fatty acid-producing bacteria in the intestinal tract of rats fed normal diet and high-fat diet, and reduced the content of endotoxin-producing bacteria.

[0042] Adopt the method of redundancy analysis (Redundancy analysis, RDA) to find out 268 OTUs relevant with berberine treatment, the result is as follows figure 2 shown. Among them, 93 OTUs (sequence 1-93 in the sequence listing) can be enriched under the action of berberine, and the other 175 OTUs (sequence 94-268 in the sequence listing) are reduced or eliminated under the action of berberine. Based on this, according to the method for improving the intestinal flora structure of the present invention, the sequence of the 16S rRNA gene V3 region of the increased intestinal bacteria has at least 95 similarities with any one of the sequences 1-93 in the sequence listing. %, 90%, 85%, 80%, 75%, or 70%. In addition, the sequence of the 16S rRNA ge...

Embodiment 3

[0045] Example 3 Berberine increases the content of short-chain fatty acids in the intestinal tract of normal and high-fat diet rats

[0046] The content of short-chain fatty acids (including acetic acid, propionic acid, butyric acid, valeric acid, isobutyric acid, isovaleric acid, etc.) in rat feces was determined by gas chromatography. The results show that oral administration of 100mg / kg of berberine can significantly increase the content of short-chain fatty acids in the intestinal tract of normal diet or high-fat diet rats, especially the increase of acetic acid and propionic acid is more significant (see image 3 ). Based on this, according to the method for improving the intestinal flora structure of the present invention, the preparation can increase the level of short-chain fatty acids in the intestinal tract.

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Abstract

The invention provides a method for improving an intestinal colony structure and an application in the preparation of medicines, nourishment, health products, foods and beverages used for adjusting the intestinal colony. A high-flux sequence test technology and a multivariate statistical method are employed to find the intestinal colony groups which are closely related with host metabolism. According to the invention, berberine is taken as a sample to establish a novel method for improving the intestinal colony structure, the method comprises the steps of selectively increasing a part of bacteria, such as short chain aliphatic acid producing bacteria and the like, and simultaneously inhibiting a part of strain, such as bacteria capable of producing endotoxin and the like, thereby the disorders of metabolism or an immunization system due to enhancement of system inflammatory diseases induced by improper diet, obesity, insulin resistance and the like can be prevented or treated. The invention also provides a method for screening medicines, compounds and foods having the analogous effects, and a composition for improving the intestinal colony structure. The application of the method can be used for exploiting the medicines, nourishment, health products, foods, beverages and the like which take the intestinal colony as a target.

Description

technical field [0001] The invention relates to a method for improving the intestinal flora structure, and its application in the process of preparing medicine, nutritional product, health product, food, beverage and the like. Background technique [0002] There are a large number of microorganisms living symbiotically in the human body, especially intestinal microorganisms are the most important "endogenous environmental factors" of the human body. There are more than 1,000 types of bacteria, the number of cells is about 10 times that of the human body's own cells, and the number of genes is about 10 times that of the human body itself. About 150 times larger than the genome. The sum of the genomes of these microorganisms is called the "microbiome" or "human metagenome", also known as the second genome of the human body. The latest view of the human body believes that the human body is a "super organism" composed of host cells and symbiotic microbial cells, especially the ...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61K36/28A61K36/288A61K36/29A61K36/42A61K36/48A61K36/489A61K36/51A61K36/515A61K36/53A61K36/539A61K36/70A61K36/708A61K36/71A61K36/718A61K36/74A61K36/744A61K36/75A61K36/756A61K36/896A61K36/8964A61K31/4375A61K31/47A23L1/29A61P1/00A23L33/00
CPCA61K36/489A61K36/756A61K36/718A61K36/744A61K36/539A61K36/185A61K36/708A61K36/288A61K36/8964A61K36/29A61K36/515A61K36/42A61K31/4375A23V2002/00A23V2200/3204A61K2300/00A61K36/75A61K36/88A61K35/74A61K35/741A61K35/745A61K36/28A61K36/48A61K36/53A61K36/59A61K36/70A61K36/71A61K36/74A23L33/30A23L33/105A61P1/00A61P1/12A61P1/14A61P19/06A61P29/00A61P3/00A61P3/04A61P3/06A61P43/00A61P3/10C12Q1/689G01N33/5088A61K31/702G01N33/48C12Q2600/136G01N2500/10
Inventor 赵立平张旭张梦晖赵宇峰庞小燕张晓君王凌华宁光李小英张翼飞
Owner SHANGHAI JIAO TONG UNIV
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