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Method for extracting refined astragaloside from astragaliradix

A technology of astragaloside IV and Astragalus membranaceus, applied in the field of extracting and refining astragaloside IV, which can solve the problems of low yield of astragaloside IV and unsuitability for large-scale production

Active Publication Date: 2012-10-24
HEBEI YILING MEDICINE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this method has applied organic solvent, is not suitable for large-scale production, and the yield of astragaloside IV is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] a. Extraction: Weigh 20kg of astragalus decoction pieces, add 240L of 80% ethanol solution each time, heat and reflux for extraction twice, each time for 3 hours, soak for 20 minutes for the first time, and filter; the extracted filtrates are combined, concentrated under reduced pressure until there is no alcohol smell, and dilute to volume 40L, get concentrated solution;

[0046] b. Alkaline hydrolysis: put the concentrated solution into the extraction tank, add 2.5L of 1mol / L NaOH solution, heat to 60°C, stir, continuously reflux for 6 hours, and dilute to 50L with distilled water to obtain an alkaline hydrolyzate;

[0047] c. The first purification: Weigh 10 kg of AB-8 resin, put it into a chromatographic column with a diameter of 20 cm, a height of 60 cm, and a volume of 19 L, and clean it with 95% ethanol and distilled water; load the alkali hydrolyzate , the adsorption flow rate is 114L / h, saturated for 3h; use 380L of distilled water to elute to remove sugar, the...

Embodiment 2

[0052] a. Extraction: Weigh 5kg of Astragalus decoction pieces, add 30L of 60% ethanol solution each time, heat and reflux for extraction 3 times, each time for 1h, soak for 40min for the first time, and filter; the extracted filtrates are combined, concentrated under reduced pressure until no alcohol smell, and dilute to volume 5L, to obtain a concentrated solution;

[0053] b. Alkaline hydrolysis: put the concentrated solution into the extraction tank, add 2.5L of 1mol / L NaOH solution, heat to 100°C, stir, continuously reflux for 2 hours, and dilute to 12.5L with distilled water to obtain an alkaline hydrolyzate;

[0054]c. The first purification: Weigh 10 kg of macroporous adsorption resin HPD100 resin, put it into a chromatographic column with a diameter of 13 cm, a height of 143 cm, and a volume of 19 L, and clean it with 95% ethanol and distilled water; load the alkali hydrolyzate , the adsorption flow rate is 114L / h, saturated for 1h; use 190L distilled water to elute s...

Embodiment 3

[0059] a. Extraction: Weigh 10kg of Radix Astragali decoction pieces, add 90L of 70% ethanol solution each time, heat and reflux for extraction 3 times, each time for 2 hours, soak for 30 minutes for the first time, and filter; the extracted filtrates are combined, concentrated under reduced pressure until there is no alcohol smell, and dilute to volume 15L, to obtain a concentrated solution;

[0060] b. Alkaline hydrolysis: put the concentrated solution into the extraction tank, add 3.75L of 1mol / L NaOH solution, heat to 80°C, stir, continuously reflux for 4 hours, and dilute to 41.7L with distilled water to obtain an alkaline hydrolyzate;

[0061] c. The first purification: Weigh 10kg macroporous adsorption resin HPD910 resin, put it into a chromatographic column with a diameter of 15cm, a height of 113cm, and a volume of 20L, and clean it with 95% ethanol and distilled water; put the alkali hydrolyzate into the sample , the adsorption flow rate is 120L / h, saturated for 2h; ...

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Abstract

The invention discloses a method for extracting refined astragaloside from astragaliradix. Astragaloside is a lanolin alcohol-form tetracyclic triterpenoid saponin compound. A Chinese herbal medicine astragaliradix is rich in astragaloside. The method utilizes a series of high-efficiency extraction, separation and purification technical processes of ethanol reflux extraction, alkaline hydrolysis, macroporous resin purification, ion-exchange resin decoloration and recrystallization to realize extraction of a target compound which is white powder and has a yield more than 0.07% and purity more than 99%. The method is simple, can be operated easily, does not produce pollution and is suitable for large-scale production.

Description

technical field [0001] The invention relates to a method for extracting and refining astragaloside IV from safflower. Background technique [0002] Astragalus membranaceus ( Fisch. ) Bge. var. mongholicus (Bge. ) Hsiao or the dried root of Astragalus membranaceus ( Fisch. ) Bge. Astragalus membranaceus is a mesophyte that grows in mountainous forest margins, shrubs and sparse forests. Distributed in the Altai Mountains in Xinjiang, Daxingan Mountains, Xiaoxing'an Mountains, Wanda Mountains, Zhangguangcai Mountains, Laoye Mountains, Changbai Mountains in the northeast of China, and the mountains in eastern Liaoning and Yanshan Mountains, Taishan Mountains, Xiaowutai Mountains, Hengshan Mountains, Wutai Mountains, Loess Plateau, Taiyue Mountains, The north of Qinling Mountains and Hengduan Mountains; Mongolian Astragalus membranaceus is an arid mesophyte, which grows in mountain grasslands, shrubs, forest margins, and ditch sides. Distributed in Baiyinxile, Xilin Gol, Keshik...

Claims

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Application Information

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IPC IPC(8): C07J53/00
Inventor 王宗权贾继明赵韶华宋剑姚道鲁
Owner HEBEI YILING MEDICINE INST
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