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Method for enriching phosphatidyl inositol from antarctic krill

A technology for enriching phosphatidylinositol and Antarctic krill, which is applied in the direction of producing fat, fat oil/fat production, etc., can solve the problems that have not been reported on Antarctic krill, and achieve the effect of high enrichment efficiency.

Inactive Publication Date: 2012-10-24
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no research report on PI in Antarctic krill

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Weigh 500g of frozen Antarctic krill (80% water content, 20% shrimp dry matter, the same below), add 600mL of 95% ethanol, stir at room temperature for 3h, pour out the supernatant and filter. The precipitate was repeatedly extracted 4 times, a total of 5 times, the extract was filtered and combined to obtain the filtrate; the filtrate was evaporated and concentrated to 10% of the volume of the combined filtrate at 60°C and -0.07MPa to obtain a concentrated extract; Add the same volume of n-hexane, shake well and let stand to separate layers, take the transparent reddish bottom layer, evaporate and remove n-hexane at 52°C and -0.08MPa to obtain 13g oily liquid. The content of phospholipids in the liquid is 2.72%, of which PI accounts for 51.92% of the phospholipids.

Embodiment 2

[0019] Weigh 500g of frozen Antarctic krill and add 550mL of 90% ethanol. The extraction was stirred at room temperature for 3 h, and the supernatant was decanted and filtered. The precipitate was extracted again 3 times, a total of 4 times, the extract was filtered and combined to obtain the filtrate; the filtrate was concentrated to 8% of the volume of the combined filtrate at 65°C and -0.09MPa to obtain a concentrated extract; With the same volume of n-hexane, shake well and let stand to separate layers, take the transparent reddish bottom layer, and evaporate the solvent at 55°C and -0.09MPa to obtain 11g of oily liquid. The content of phospholipids in the liquid is 2.70%, of which PI accounts for 50.5% of the phospholipids.

Embodiment 3

[0021] Weigh 500g of frozen Antarctic krill, add 650mL of absolute ethanol, stir and extract at room temperature for 1h, pour out the supernatant and filter. The precipitation was extracted again 5 times, for a total of 6 times. The extracts were filtered and combined to obtain the filtrate; the filtrate was concentrated to 10% of the volume of the combined filtrate at 60°C and -0.08MPa to obtain a concentrated extract; the same volume of n-hexane was added to the concentrate, shaken and allowed to stand for stratification , get the transparent reddish bottom layer liquid, at 50 DEG C, evaporate solvent under-0.07MPa, obtain 12g oily liquid. The content of phospholipids in the liquid is 2.76%, of which PI accounts for 51.7% of the phospholipids.

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Abstract

The invention discloses a method for enriching phosphatidyl inositol from antarctic krill. The method includes the steps: (1) adding 1 kilogram of frozen antarctic krill to 1-1.3 liters of ethanol solution with the volume fraction of 90-100%, stirring to extract for 4-6 times under natural conditions, keeping extraction each time for 1-3h, and filtering and combining extracting solutions so as to obtain filtrate; (2) concentrating the obtained filtrate under the pressure of negative 0.07-negative 0.09Mpa at the temperature of 60-65 DEG C until 8-10% of the volume of the filtrate remains so as to obtain concentrated extracting solution; (3) adding isometric normal hexane into the obtained concentrated extracting solution, uniformly mixing and statically layering so as to obtain transparent reddish bottom solution; and (4) removing the normal hexane from the obtained bottom solution by vaporizing under the pressure of negative 0.07-negative 0.09Mpa at the temperature of 50-55 DEG C so as to obtain oily solution, namely, a phosphatidyl-inositol-enriched product, wherein the product comprises 2.70-2.76% of phospholipid, and the phosphatidyl inositol accounts for 50.5-51.92% of the phospholipid. Re-extraction, separation, purification and the like can be further performed on the basis so as to obtain the phosphatidyl inositol.

Description

technical field [0001] The invention relates to a method for enriching phosphatidylinositol from Antarctic krill. Background technique [0002] Antarctic krill is a kind of krill that lives in the waters of Antarctica in the Antarctic Ocean. It lives in clusters, sometimes with a density of 10,000-30,000 per M 3 , they may be the most successful animal species on earth, according to statistics, there are about 500-500000000 tons (there are big differences due to different statistical methods). [0003] At present, the Antarctic krill fishery is about 100,000 tons per year, 80% of which are caught by Japan, because the baleen whales that mainly eat krill in the Southern Ocean have also been wiped out. Relevant scientists have estimated that there are about 150 million tons of krill remaining due to the decline of cetacean resources every year based on the cetacean's food intake on krill during the peak period. The annual total fishery output in the world is 99 million tons,...

Claims

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Application Information

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IPC IPC(8): C11B1/02C11B1/10
Inventor 刘代成马夫翠
Owner SHANDONG NORMAL UNIV
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