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4, 5 diamino 1-pyrazole sulphate degradation bacteria and bacterium agent produced by same

A technology of pyrazole sulfate and diamino, applied in the biological field, can solve the problems of pollution, comprehensive management of hazards, etc., and achieve the effects of convenient use, low production and use cost, and increased added value

Active Publication Date: 2013-06-26
海宁经开产业园区开发建设有限公司
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Problems solved by technology

The environmental pollution of 4,5 diamino-1-pyrazole sulfate has seriously endangered the health of human beings. The comprehensive management of its environment, the degradation behavior of 4,5 diamino-1-pyrazole sulfate in the environment has never been See more mature technology

Method used

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  • 4, 5 diamino 1-pyrazole sulphate degradation bacteria and bacterium agent produced by same
  • 4, 5 diamino 1-pyrazole sulphate degradation bacteria and bacterium agent produced by same
  • 4, 5 diamino 1-pyrazole sulphate degradation bacteria and bacterium agent produced by same

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Embodiment Construction

[0019] 4,5-diamino-1-pyrazole sulfate-degrading bacteria, preserved in the General Microbiology Center (CGMCC) of China Microbiological Culture Collection Management Committee (CGMCC) on March 2, 2012, address: No. 1, Beichen West Road, Chaoyang District, Beijing No. 3, the deposit number is CGMCC NO.5834, and the classification name is Candida rugopelliculosa (Candida rugopelliculosa).

[0020] 1. Isolation and identification of strains

[0021] Take 10g of contaminated soil, put it in 100ml of sterile water, and shake it for 5min to get the soil bacteria suspension; take 5ml of the soil bacteria suspension, add it to 100ml of inorganic salt medium [NH 4 NO 3 : 407mg / L; KH 2 PO 4 : 98mg / L; K 2 HPO 4 : 33mg / L; NaCl: 30mg / L; MgSO 4 : 200mg / L; pH7.0], add 4,5 diamino 1-pyrazole sulfate as a carbon source, place it at 30°C, and cultivate it on a shaker at 150r / min to obtain the enriched solution, and continuously add 4,5 diamino Amino 1-pyrazole sulfate medium plates were ...

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Abstract

The invention discloses a 4, 5 diamino 1-pyrazole sulphate degradation bacteria and a bacterium agent produced by same. A used bacterial strain is a bacterial strain L-1 of Candidarugopelliculosa, the preservation number is CGMCCNO.5834, and the main biological characteristics are that the bacterial strain is facultative and aerobic, is white, is in an oval shape to a sausage shape, and has the size of 3.0-5.0*5.6-10.2mum; a bacterial colony is in a puffy shape, is light brown, is lustreless, and has striatedripples and etched edges; pseudohypha is generated when corn meal agar Dalmau is subjected to plate cultivation; and growing is carried out by taking 4, 5 diamino 1-pyrazole sulphate or glucose as a carbon source, and the optimum growing temperature is 30 DEG C. The degradation rate of the 4, 5 diamino 1-pyrazole sulphate can reach more than 70 percent under the condition of shake-flask culture in a lab. The degradation bacterium agent has the advantages of low cost, convenience in use and good removal effect, and is suitable for pollution treatment of water and soil.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a 4,5-diamino-1-pyrazole sulfate-degrading bacteria and a bacterial agent produced therefrom, which degrades dyes by means of microorganisms and is suitable for the treatment of environmental pollution. Background technique [0002] With the development of modern synthetic industry, a large number of xenobiotics (Xenobiotics) have entered into industrial wastewater and urban sewage. Due to their structural complexity and biological unfamiliarity, the types and quantities of indigenous microorganisms that can degrade and transform pollutants in polluted environments Fewer or even no indigenous microorganisms that can degrade some specific pollutants. Even if indigenous microbial communities can degrade pollutants, they can't degrade them quickly enough to stop the spread of pollutants. When rapid response is very important, small numbers of indigenous microbial communities...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12R1/72A62D101/28
Inventor 刘秀艳张迹
Owner 海宁经开产业园区开发建设有限公司
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