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Method and device for detecting biochemical oxygen demand

A technology of biochemical oxygen demand and first oxygen content, which is applied in the direction of material electrochemical variables, can solve the problems that cannot play an important role, cannot respond to changes in organic matter in time, and cannot provide real-time BOD content, etc., to achieve measurement Accurate results and labor-saving effects

Active Publication Date: 2012-11-28
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, before the measurement, the sample and the blank solution need to be subjected to constant temperature treatment, and the two should be effectively saturated with air, so that the dissolved oxygen content of the two can be as consistent as possible, so that the BOD content of the water sample cannot be given in real time, and cannot be obtained in time. It can not play an important role in the emergency warning of water pollution

Method used

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  • Method and device for detecting biochemical oxygen demand
  • Method and device for detecting biochemical oxygen demand
  • Method and device for detecting biochemical oxygen demand

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0115] according to figure 1 Schematic diagram of the preparation of the microbial membrane reactor is shown.

[0116] Turn on the constant temperature water bath device 10 and adjust the temperature to 30°C. Inject the activated sludge water sample into the water sample container 1, and carry out air saturation to the activated sludge water sample at a rate of 3.0 L / min. Place a glass reactor 7 with a length of 105.0 cm and an inner diameter of 2.0 mm in a constant temperature water bath, and use a peristaltic pump 3 to pass the activated sludge water sample through the first sampling tube 2 and the peristaltic pump 3 at a rate of 1.0 mL / min. Pump 3, second injection tube 4, oxygen electrode E 1 5. The third sampling tube 6 reaches the reactor 7, and then passes through the fourth sampling tube 8 and the oxygen electrode E 2 9, finally discharged from the sample outlet pipe 15. At the same time, the activated sludge water sample is continuously added to the water sample c...

Embodiment 2

[0118] according to figure 1 As shown in the schematic diagram, the microbial membrane reactor 7 is removed from the system, and replaced by a glass tube with equal length and equal inner diameter. 2 9-phase connection for oxygen electrode calibration.

[0119] The oxygen electrode E 1 5 with oxygen electrode E 2 9 is connected to the first electrochemical workstation 11 and the second electrochemical workstation 12 through the first data line 13 and the second data line 14 respectively. Water sample container 1, peristaltic pump 3, oxygen electrode E 1 5. Glass tube, oxygen electrode E 2 9 are respectively connected through the first sampling tube 2, the second sampling tube 4, the third sampling tube 6, and the fourth sampling tube 8, and are discharged by the sampling tube 15. Oxygen electrode E 1 5. Glass tube, oxygen electrode E 2 9 placed in a constant temperature water bath device 10. The first electrochemical workstation 11 and the second electrochemical workst...

Embodiment 3

[0128] according to figure 1 The device used to detect biochemical oxygen demand obtained in the schematic diagram shown in the figure is to measure a series of concentrations of glucose and glutamic acid solutions to obtain a standard curve.

[0129] Turn on the constant temperature water bath device 10 and adjust the temperature to 30°C. Inject tap water into the water sample container 1, and use the peristaltic pump 3 to pass the tap water through the first sampling tube 2, the peristaltic pump 3, the second sampling tube 4, and the oxygen electrode E at a rate of 1.0 mL / min. 1 5. The third sampling tube 6, microbial membrane reactor 7, the fourth sampling tube 8, oxygen electrode E 2 9, finally discharged from the sample outlet pipe 15. Oxygen electrode E 1 5 and oxygen electrode E 2 9 When the monitored output current is stable, the current values ​​are 632nA and 512nA respectively. Inject the glucose glutamic acid solution with a certain BOD content into the water s...

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Abstract

The invention provides a detection method for biochemical oxygen demand, which comprises the following steps: a), carrying out microculture on a water sample containing microorganisms to obtain a microbial film; b), enabling a water sample to be detected to sequentially pass through a first oxygen electrode, the microbial film in the step (a) and a second oxygen electrode, and obtaining first oxygen current and second oxygen current of the water sample to be detected through the detection of the oxygen electrodes; c), obtaining first oxygen content and second oxygen content of the water sample to be detected according to a calibration curve of the first oxygen current and the predetermined first oxygen electrode and a calibration curve of the second oxygen current and the predetermined second oxygen electrode; d), obtaining a difference value of the oxygen content of the water sample to be detected according to the first oxygen content and the second oxygen content of the water sample to be detected obtained in the step (c); and e), obtaining the biochemical oxygen demand of the sample to be detected according to the difference value of the oxygen content of the water sample to be detected in the step (d) and a predetermined standard curve. The oxygen content change obtained by the detected method can reflect the degree that a water body is polluted and play a role of early warning.

Description

technical field [0001] The invention relates to the technical field of environmental protection, in particular to a method and device for detecting biochemical oxygen demand. Background technique [0002] Biochemical oxygen demand (BOD) refers to the amount of dissolved oxygen consumed by microorganisms to decompose certain oxidizable substances in water, especially organic pollutants. BOD is an important indicator for analyzing the content of organic pollutants in water bodies, and is one of the most important parameters in routine water quality testing. The higher the value, the more organic pollutants in the water and the more serious the pollution. The international standard method for determining BOD is the five-day culture method (i.e. BOD 5 The specific process is: inoculate the water sample to be tested with broad-spectrum microorganisms, dilute it in a certain proportion, and culture it at a constant temperature in the dark for 5 days, and calculate the biochemical...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
Inventor 刘长宇董绍俊
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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