Device for quickly detecting pesticide residues in fruits and vegetables, and application thereof

Abstract

The invention discloses a device for quickly detecting pesticide residues in fruits and vegetables, which comprises a color developing pool, a waste liquid pool, a sample liquid supply device, a color developing liquid supply device, a buffer liquid supply device, a substrate supply device, a sample liquid pump, a substrate pump, a color developing liquid pump, a buffer liquid pump, a valve system, a reactor and an electronic monitoring and control system. The device for quickly detecting pesticide residues in fruits and vegetables is used for quickly detecting the pesticide residues in fruits and vegetables, so that the interference of the color of a sample liquid of fruits and vegetables to be detected to the detection results can be fully eliminated. And meanwhile, the enzyme inhibition reaction and the enzyme-catalyzed substrate hydrolysis reaction are carried out in the same closed reactor, therefore, the impact of environment factor change is controlled to the minimum extent, and the detection results are relatively more accurate.

Description

technical field [0001] The invention relates to a detection device for pesticide residues, in particular to a device for rapid detection of pesticide residues in fruits and vegetables and its application in the aspect of rapid detection of pesticide residues in fruits and vegetables. Background technique [0002] The detection of organophosphorus and carbamate pesticide residues by enzyme inhibition has a history of more than 50 years. The basic principle is that organophosphorus and carbamate pesticides can inhibit the activity of acetylcholinesterase in the central and peripheral nervous systems of insects. According to this toxicological principle, the current rapid detection of organophosphorus and carbamate pesticide residues in fruits and vegetables mainly uses cholinesterase as the detection enzyme, and at the same time uses photometry to detect the degree of inhibition of the enzyme, so as to judge Determine the level of pesticide residues in the sample. [0003] H...

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Problems solved by technology

[0003] However, because most of the rapid detection is realized in the form of kits and simple photometers on the market at present, the colored fruit and vegetable sample liquid is mixed with the liquid enzyme inhibition and subsequent catalytic reaction system during the detection, which seriously interferes with the photometric method. Accuracy of test results

Therefore, when the enzyme inhibition method is used to quickly detect pesticide residues in fruits and vegetables, usually the liquid detection enzyme cannot be effectively separated from the fruit and vegetable sample liquid after inhibition, resulting in inaccurate detection results and even serious consequences of misjudgment of conclusions.

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