Device for quickly detecting pesticide residues in fruits and vegetables, and application thereof

A technology for pesticide residues, fruits and vegetables, which is applied in the field of pesticide residue detection devices, can solve the problems of separation, misjudgment of conclusions, and interference with the accuracy of photometric detection results, so as to achieve accurate detection results and eliminate interference.

Active Publication Date: 2012-12-12
上海林默精密仪器有限公司
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AI-Extracted Technical Summary

Problems solved by technology

[0003] However, because most of the rapid detection is realized in the form of kits and simple photometers on the market at present, the colored fruit and vegetable sample liquid is mixed with the liquid enzyme inhibition and subsequent catalytic reaction system during the detection, which seriously interferes with the photometric method. Accuracy of te...
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Method used

Chromogenic pool 6, buffer supply device 20, the reactor 5 used for carrying immobilized fruit and vegetable pesticide residue detection enzyme and the pipeline connected with each other are provided with temperature sensor and heating device respectively, in whole testing process In the process, the central processing unit always issues instructions to the temperature control module to keep the piping system, reactor and chromogenic pool flowing through the above-m...
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Abstract

The invention discloses a device for quickly detecting pesticide residues in fruits and vegetables, which comprises a color developing pool, a waste liquid pool, a sample liquid supply device, a color developing liquid supply device, a buffer liquid supply device, a substrate supply device, a sample liquid pump, a substrate pump, a color developing liquid pump, a buffer liquid pump, a valve system, a reactor and an electronic monitoring and control system. The device for quickly detecting pesticide residues in fruits and vegetables is used for quickly detecting the pesticide residues in fruits and vegetables, so that the interference of the color of a sample liquid of fruits and vegetables to be detected to the detection results can be fully eliminated. And meanwhile, the enzyme inhibition reaction and the enzyme-catalyzed substrate hydrolysis reaction are carried out in the same closed reactor, therefore, the impact of environment factor change is controlled to the minimum extent, and the detection results are relatively more accurate.

Application Domain

Technology Topic

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  • Device for quickly detecting pesticide residues in fruits and vegetables, and application thereof
  • Device for quickly detecting pesticide residues in fruits and vegetables, and application thereof
  • Device for quickly detecting pesticide residues in fruits and vegetables, and application thereof

Examples

  • Experimental program(1)
  • Effect test(1)

Example Embodiment

[0060] Example 1
[0061] A rapid detection device for pesticide residues in fruits and vegetables, the schematic diagram of its structure is as follows figure 1 As shown, it includes a color developing tank 6, a waste liquid tank 8, a sample liquid supply device 22, a color developing liquid supply device 21, a buffer supply device 20, a substrate supply device 19, a sample liquid pump 4, a substrate pump 3, a display Color solution pump 1, buffer solution pump 2, valve system and an electronic measurement and control system. In addition, it also includes a reactor 5 for carrying enzymes used in the detection of immobilized fruit and vegetable pesticide residues;
[0062] The sample liquid pump 4, the substrate pump 3, the color developing liquid pump 1, and the buffer liquid pump 2 all use plunger pumps, and are respectively provided with corresponding position sensors, and each plunger pump is started by its Corresponding stepper motor to control;
[0063] The valve system includes a three-way valve I15, a three-way valve III13, a three-way valve II14, a three-way valve IV12, a three-way valve V16, a two-way valve 17, and a two-way valve II18, and each valve adopts a solenoid valve.
[0064] The reactor 5 used to carry the enzymes used for the detection of pesticide residues in immobilized fruits and vegetables has a schematic structural diagram such as figure 2 As shown, the inlet and outlet of the closed reactor are respectively equipped with filter devices 51 and 52, and the filter devices 51 and 52 are metal filter screens with 60-80 mesh;
[0065] The reactor 5 used to carry the enzymes used for the detection of immobilized fruit and vegetable pesticide residues has a cylindrical reactor with a diameter-to-height ratio of 1:5, that is, a diameter of 6mm, a height of 30mm, and its material is stainless steel;
[0066] The fruit and vegetable sample liquid supply device 22 is respectively connected to the sample liquid pump 4 and the inlet of the reactor 5 for carrying the enzyme used in the detection of the immobilized fruit and vegetable pesticide residue through the three-way valve I15;
[0067] The buffer supply device 20 is respectively connected to the buffer pump 2 and the inlet of the reactor 5 for carrying the enzyme used for the detection of immobilized fruit and vegetable pesticide residues through a three-way valve III13;
[0068] The substrate supply device 19 is respectively connected to the substrate pump 3 and the inlet of the reactor 5 for carrying the enzyme used for detecting the immobilized fruit and vegetable pesticide residue through the three-way valve II14;
[0069] The color developing liquid supply device 21 is respectively connected to the color developing pump 1 and the color developing tank 6 through a three-way valve IV12;
[0070] After the outlet of the enzyme reactor 5 used for the detection of the residue of immobilized fruit and vegetable pesticides passes through the three-way valve V16, one of them passes through the two-way valve I17 and then enters the waste liquid tank 8, all the way into the color developing tank 6, the color developing tank 6 The outlet at the bottom is also provided with a colorimetric channel 7. One side of the colorimetric channel is equipped with a photoelectric emission tube 10 with a wavelength of 410nm, and the corresponding side is equipped with a photoelectric receiving tube 11, that is, light absorption is performed in the colorimetric channel 7 Value analysis, at the same time, the pipeline from the colorimetric channel 7 is provided with a two-way valve II18 and enters the waste liquid pool 8 through the pipeline. The waste liquid pool 8 is provided with a discharge port and a valve 9 is provided on the discharge port. The valve 9 is manual or automatic, which realizes the discharge of the waste liquid in the waste liquid pool;
[0071] The control principle of the electronic measurement and control system is as follows image 3 , The system is mainly composed of signal acquisition and amplification module, human-computer interaction module, temperature control module, pump valve control module and central processing unit (CPU);
[0072] The temperature control module includes a temperature sensor and a heating device;
[0073] The pump valve control module is for the above-mentioned sample liquid pump 4, substrate pump 3, color developing liquid pump 1, buffer pump 2, three-way valve I15, three-way valve III13, three-way valve II14, three-way valve IV12, and three-way valve. Modules for the two-way valve V16, the two-way valve 17, and the two-way valve II18 to perform start-stop control;
[0074] The working route of the above electronic measurement and control system is as follows:
[0075] The human-computer interaction module issues instructions to the central processing unit, and the central processing unit opens the pump valve control module according to the instructions issued to bring the fruit and vegetable sample solution, buffer, substrate solution or color developing solution to be tested through the corresponding pump and valve system. To the light source, that is, the photoelectric emission tube 10 and the signal detector, that is, the photoreceiving tube 11, the light emitted by the photoelectric emission tube 10 is collected by the signal detector, which is the photoelectric receiving tube 11, and amplified by The module converts the collected optical signals into electrical signals. The electrical signals are amplified by the amplifying circuit and sent to the central processing unit. The central processing unit is responsible for processing and comparing the signals, and finally outputs the obtained detection results to the human-computer interaction module On the display;
[0076] The color developing tank 6, the buffer supply device 20, the reactor 5 used to carry the enzyme used in the detection of immobilized fruit and vegetable pesticide residues, and the pipes connected to each other are respectively equipped with temperature sensors and heating devices. During the whole test process, the central The processor always gives instructions to the temperature control module to keep the pipe system, reactor and color developing tank through which the above-mentioned fruit and vegetable sample solution, buffer, substrate solution or color developing solution to be tested is at a constant temperature, thereby reducing and eliminating Environmental temperature changes cause interference to detection. The temperature control of the rapid detection device for pesticide residues in fruits and vegetables is controlled by PID control to accurately control the temperature of key points, and the control accuracy is within ±0.5°C.
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Description & Claims & Application Information

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the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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